Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-09T08:55:54.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological and molecular analyses of the spiruroid nematode, Falcaustra araxiana Massino, 1924 (= Spironoura araxiana) from the European pond turtle (Emys orbicularis)

Published online by Cambridge University Press:  01 April 2016

M. Rajabloo ,
H. Sharifiyazdi ,
F. Namazi ,
H. Shayegh ,
E. Rakhshandehroo  and
G. Farjanikish
M. Rajabloo
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
H. Sharifiyazdi*
Affiliation:
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
F. Namazi
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
H. Shayegh
Affiliation:
School of Veterinary Medicine, Shiraz University, Shiraz, Iran
E. Rakhshandehroo
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
G. Farjanikish
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran
*
*E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

There is little information on the phylogenetic position and life cycle of family Kathlaniidae. Falcaustra araxiana is a member of this family which infects the large intestine of the European pond turtle (Emys orbicularis). In the present study, morphological data and molecular analyses based on the 18S rDNA were performed on different types of F. araxiana originating from the large intestine and gastric nodules in the turtle. Morphological data revealed both larvae and adult stages in the gastric nodules. In addition, all nematodes recovered in the large intestine were adult worms. GenBank accession numbers KM200715 and KM200716 were provided for adult F. araxiana located in the intestine and stomach, respectively, of E. orbicularis. The results of sequencing proved that these two types are completely similar. Accordingly, it can be hypothesized that nodule formation is a part of the life cycle of the parasite or a survival strategy. Furthermore, F. araxiana develops to the adult stage in the gastric mucosa prior to migrating to the large intestine. Phylogenetic analysis revealed that F. araxiana unexpectedly branched away from other members of the superfamily Seuratoidea (Truttaedacnitis truttae, Cucullanus robustus and C. baylisi) and showed a closer relationship with Paraquimperia africana, a member of the Ascaridoidea. It seems that phylogenetic reconstruction for the present parasite needs more detailed morphology, life cycle and molecular studies.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 3 , May 2017 , pp. 356 - 359
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anderson, R.C. (2000) Nematode parasites of vertebrates: Their development and transmission. 2nd edn. Wallingford, CABI Publishing.CrossRefGoogle Scholar
Bartlett, C.M. & Anderson, R.C. (1985) Larval nematodes (Ascaridida and Spirurida) in the aquatic snail, Lymnaea stagnalis . Journal of Invertebrate Pathology 46, 153–159.Google Scholar
Brianti, E., Gaglio, G., Giannetto, S., Annoscia, G., Latrofa, M.S., Dantas-Torres, F., Traversa, D. & Otranto, D. (2012) Troglostrongylus brevior and Troglostrongylus subcrenatus (Strongylida: Crenosomatidae) as agents of broncho-pulmonary infestation in domestic cats. Parasites & Vectors 5, 178.CrossRefGoogle ScholarPubMed
Bursey, C.R. & Rivera, S. (2009) New species of Falcaustra (Nematoda; Ascaridida: Kathlaniidae) in the impressed tortoise, Manouria impressa (Testudines: Testudinidaae). Comparative Parasitology 76, 141–148.Google Scholar
Bursey, C.R., Sung, Y.H. & Goldberg, S.R. (2012) New species of Falcaustra (Nematoda: Kathlaniidae) in Platysternon megacephalum (Testudines: Platysternidae) from China. Journal of Parasitology 98, 569–571.CrossRefGoogle ScholarPubMed
Hasegawa, H., Sato, A., Kai, M. & Uchida, A. (2013) Helminth parasites of bullfrogs, Lithobates catesbeianus (Shaw, 1802), in Kanto District, Japan, with special reference to those introduced from North America. Japanese Journal of Veterinary Parasitology 12, 1–10.Google Scholar
Moravec, F., Huffman, D.G. & Swim, D.J. (1995) The first record of fish as paratenic hosts of Falcaustra spp. (Nematoda: Kathlaniidae). Journal of Parasitology 81, 809–812.Google Scholar
Shayegh, H., Rajabloo, M., Gholamhosseini, A., Mootabi Alavi, A., Salarian, P. & Zolfaghari, A. (2016) Endohelminths of European pond turtle Emys orbicularis in Southwest Iran. Journal of Parasitic Disease 40, 194–198.Google Scholar
Soulsby, E.J.L. (1982) Helminths, arthropods and protozoa of domesticated animals. 7th edn. London, Bailliere Tindall.Google Scholar
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.CrossRefGoogle ScholarPubMed
Wharton, D.A. (1986) A functional biology of nematodes. Baltimore, Maryland, USA, The Johns Hopkins University Press.Google Scholar
Yamaguti, S. (1962) Systema Helminthum. Vol. 3, The nematodes of vertebrates. New York, Wiley InterScience.Google Scholar
Zajac, A.M. & Conboy, G.A. (2012) Veterinary clinical parasitology. 8th edn. Ames, Iowa, USA, Wiley-Blackwell.Google Scholar