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Molecular identification of the trematode Paragonimus in faecal samples from the wild cat Prionailurus bengalensis in the Da Krong Nature Reserve, Vietnam

Published online by Cambridge University Press:  21 September 2015

P.N. Doanh ,
H.V. Hien ,
L.A. Tu ,
N. Nonaka ,
Y. Horii  and
Y. Nawa
P.N. Doanh
Affiliation:
Institute of Ecology and Biological Resources, Viet Nam Academy of Science and Technology, Hanoi, Viet Nam
H.V. Hien
Affiliation:
Institute of Ecology and Biological Resources, Viet Nam Academy of Science and Technology, Hanoi, Viet Nam
L.A. Tu
Affiliation:
Vietnam National Lung Hospital, Hanoi, Viet Nam
N. Nonaka
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture and Center for Animal Disease Control, University of Miyazaki, Japan
Y. Horii*
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture and Center for Animal Disease Control, University of Miyazaki, Japan
Y. Nawa
Affiliation:
Research Affairs Office, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
*
*Fax: +81-0985-58-7276 E-mail: [email protected]
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Abstract

Conventional identification of Paragonimus species and their natural definitive hosts is based on the morphological features of adult parasites isolated from the lungs of wild mammalian hosts. However, wild animals are protected by strict regulations and sampling is not always possible. Recently, molecular techniques have been developed to identify the internal transcribed spacer (ITS) sequences of Paragonimus eggs in faeces/sputum of human patients. Also, mammalian hosts can be identified using the D-loop sequence of mitochondrial DNA in faecal samples. In this study, we used molecular techniques on faeces from wild animals collected in Da Krong Nature Reserve, Quang Tri province, central Vietnam, where Paragonimus metacercariae are highly prevalent in mountain crabs, to identify Paragonimus species and their natural definitive hosts. The results indicated that wild cats, Prionailurus bengalensis, were infected with at least three different Paragonimus species, P. westermani, P. skrjabini and P. heterotremus. Because all of these species can infect humans in Asian countries, human paragonimiasis should be considered in this area.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 6 , November 2016 , pp. 658 - 662
Copyright
Copyright © Cambridge University Press 2015 

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References

Blair, D., Xu, Z.B. & Agatsuma, T. (1999) Paragonimiasis and the genus Paragonimus . Advances in Parasitology 42, 113–222.Google Scholar
Blair, D., Agatsuma, T. & Wang, W.L. (2007) Paragonimiasis. pp. 117–150 in Murrell, K.D. & Fried, B. (Eds) World class parasites vol. 11, Food-borne parasitic zoonoses. New York, Springer.Google Scholar
Bowles, J., Blair, D. & McManus, D.P. (1995) A molecular phylogeny of the human schistosomes. Molecular Phylogenetic Evolution 4, 103–109.Google Scholar
Chai, J.Y., Sohn, W.M., Chung, H.L., Hong, S.T. & Lee, S.H. (1990) Metacercariae of Pharyngostomum cordatum found from the European grass snake, Rhabdophis tigrina, and its experimental infection to cats. Korean Journal of Parasitology 28, 175–181.Google Scholar
Chan, W.S., Wu, B., Blair, D., Zhang, Y.N., Hu, L., Chen, Z.H., Chen, M.G., Feng, Z. & George, M.D. (2000) Gene sequencing for identification of Paragonimus eggs from human cases. Chinese Journal of Parasitology and Parasitic Disease 18, 213–215.Google Scholar
De, N.V., Murrell, K.D., Cong, L.D., Cam, P.D., Chau, L.V. & Toan, N.D. (2003) The food-born trematode zoonoses of Viet Nam. Southeast Asian Journal of Tropical Medicine and Public Health 34, 12–34.Google Scholar
Doanh, P.N., Shinohara, A., Horii, Y., Habe, S., Nawa, Y., The, D.T. & Le, N.T. (2007a) Morphological and molecular identification of two Paragonimus spp., of which metacercariae concurrently found in a land crab, Potamiscus tannanti, collected in Yenbai Province, Viet Nam. Parasitology Research 100, 1075–1082.CrossRefGoogle Scholar
Doanh, P.N., Shinohara, A., Horii, Y., Habe, S., Nawa, Y. & Le, N.T. (2007b) Description of a new lung fluke species, Paragonimus vietnamensis sp. nov. (Trematoda: Paragonimidae), found in Central Viet Nam. Parasitology Research 101, 1495–1501.Google Scholar
Doanh, P.N., Shinohara, A., Horii, Y., Habe, S., Nawa, Y. & Le, N.T. (2008) Discovery of Paragonimus proliferus in Central Viet Nam and their molecular phylogenetic status among genus Paragonimus . Parasitology Research 102, 677–683.CrossRefGoogle Scholar
Doanh, P.N., Shinohara, A., Horii, Y., Habe, S. & Nawa, Y. (2009a) Discovery of Paragonimus westermani in Viet Nam and its molecular phylogenetic status in P. westermani complex. Parasitology Research 104, 1149–1155.Google Scholar
Doanh, P.N., Shinohara, A., Horii, Y., Yahiro, S., Habe, S., Vannavong, N., Strobel, M., Nakamura, S. & Nawa, Y. (2009b) Morphological differences and molecular similarities between Paragonimus bangkokensis and P. harinasutai. Parasitology Research 105, 429–439.Google Scholar
Doanh, P.N., Thach, D.T.C., Dung, D.T., Shinohara, A., Horii, Y. & Nawa, Y. (2011) Human paragonimiasis in Viet Nam: epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients’ sputum. Parasitology International 60, 534–537.Google Scholar
Doanh, P.N., Hien, H.V., Nonaka, N., Horii, Y. & Nawa, Y. (2012) Co-existence of Paragonimus harinasutai and Paragonimus bangkokensis metacercariae in fresh water crab hosts in central Viet Nam with special emphasis on their close phylogenetic relationship. Parasitology International 61, 399–404.Google Scholar
Doanh, P.N., Hien, H.V., Nonaka, N., Horii, Y. & Nawa, Y. (2013a) Genetically variant populations of Paragonimus proliferus Hsia & Chen, 1964 from central Vietnam. Journal of Helminthology 87, 141–146.Google Scholar
Doanh, P.N., Horii, Y. & Nawa, Y. (2013b) Paragonimus and paragonimiasis in Vietnam: an update. Korean Journal of Parasitology 51, 621–627.Google Scholar
Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Hernández, P.E. & Martin, R. (2007) PCR identification of meats from chamois (Rupicapra rupicapra), pyrenean ibex (Capra pyrenaica) and mouflon (Ovis ammon) targeting specific sequences from the mitochondrial D-loop. Meat Science 76, 644–652.CrossRefGoogle ScholarPubMed
Kino, H., De, N.V., Vien, H.V., Chuyen, L.T. & Sano, M. (1995) Paragonimus heterotremus Chen et Hsia, 1964 found from a dog in Vietnam. Japanese Journal of Parasitology 44, 470–472.Google Scholar
Kong, Y, Doanh, P & Nawa, Y (2015) Paragonimiasis. pp. 445–462 in Xiao, L., Ryan, U. & Feng, Y.Y. (Eds) Biology of foodborne parasites. London, CRC Press.Google Scholar
Kumar, D., Singh, S.P., Umapathi, V., Nagappa, K. & Gaurav, A. (2011) Identification of buffalo (Bubalus bubalis) meat using PCR targeting mithochondrial D-Loop gene. Buffalo Bulletin 30, 170–176.Google Scholar
Le, T.H., De, N.V., Blair, D., McManus, D.P., Kino, H. & Agatsuma, T. (2006) Paragonimus heterotremus Chen et Hsia, 1964 in Viet Nam: A molecular identification and relationships of isolates from different hosts and geographical origins. Acta Tropica 98, 25–33.Google Scholar
Manh, N.D., Dang, N.X. & Nghia, N.X. (2009) Conservation importance of mammal fauna in Da Krong Nature Reserve, Quang Tri province, Vietnam. Vietnam Journal of Biology 31, 42–50.Google Scholar
Miyazaki, I. (1991) An illustrated book of helminthic zoonoses. 1st edn. Tokyo, International Medical Foundation of Japan.Google Scholar
Nawa, Y., Thaenkham, U., Doanh, P.N. & Blair, D. (2014) Paragonimus westermani and Paragonimus species. pp. 179–188 in Motarjemi, Y. (Ed.) Encyclopedia of food safety. Vol. 2. Amsterdam, Elsevier.Google Scholar
Nonaka, N., Sano, T., Inoue, T., Armua, M.T., Fukui, D., Katakura, K. & Oku, Y. (2009) Multiplex PCR system for identifying the carnivore origins of feces for an epidemiological study on Echinococcus multilocularis in Hokkaido, Japan. Parasitology Research 106, 75–83.CrossRefGoogle ScholarPubMed
Odermatt, P., Habe, S., Maichanh, S., Tran, D.S., Duong, V. & Zhang, W. (2007) Paragonimiasis and its intermediate hosts in a transmission focus in Laos People's Democratic Republic. Acta Tropica 103, 108–115.Google Scholar
Parkanyi, V., Ondruska, L., Vasicek, D. & Slamecka, J. (2014) Multilevel D-loop PCR identification of hunting game. Applied and Translational Genomics 3, 1–7.CrossRefGoogle ScholarPubMed
Randi, E. (2000) Mitochondrial DNA. pp. 136–167 in Baker, A.J. (Ed.) Molecular methods in ecology. Malden, Blackwell Science.Google Scholar
Singh, T.S., Sugiyama, H., Umehara, A., Hiese, S. & Khalo, K. (2009) Paragonimus heterotremus infection in Nagaland: a new focus of Paragonimiasis in India. Indian Journal of Medical Microbiology 27, 123–127.CrossRefGoogle ScholarPubMed
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.Google Scholar
Tin, D.V. & Huynh, D.H. (1994) Checklist of mammals in Vietnam. Hanoi, Science and Technics Publishing House (in Vietnamese).Google Scholar
Vien, C.V. (1997) Some results of research on epidemiology, parasite, pathogen and prevention of paragonimiasis in Sin Ho, Lai Chau. Proceedings of the Conference on Science, Technology and Environment in North Mountainous Provinces 5, 81–84 (in Vietnamese).Google Scholar
Wallace, F.G. (1939) The life cycle of Pharyngostomum cordatum (Diesing) Ciurea (Trematoda: Alariidae). Transactions of the American Microscopical Society 58, 49–61.Google Scholar
Xie, B.W., Yang, Y.S., Li, Q.Z. & Tang, L.Y. (1985) Rana boulengeri as the paratenic host of Pagumogonimus skrjabini . Chinese Journal of Parasitology and Parasitic Disease 3, 157 (in Chinese).Google Scholar