Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-20T00:49:10.740Z Has data issue: false hasContentIssue false

Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Paraná River, Brazil

Published online by Cambridge University Press:  25 November 2015

L.S. Gasques*
Affiliation:
Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR, CEP 87502-210, Brazil Programa de pós graduação em Biologia Comparada, Centro de Ciências Biológicas, Universidade Estadual de Maringá, Av. Colombo, 5790, Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil
R.J. Graça
Affiliation:
Programa de pós graduação em Biologia Comparada, Centro de Ciências Biológicas, Universidade Estadual de Maringá, Av. Colombo, 5790, Jardim Universitário, Maringá, PR, CEP 87020-900, Brazil
S.M.A.P. Prioli
Affiliation:
Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá, Av. Colombo 5790, Bloco G-90, Maringá, PR, CEP 87020-900, Brazil Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Av. Colombo 5790, Bloco H-67, Maringá, PR, CEP 87020-900, Brazil
R.M. Takemoto
Affiliation:
Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá, Av. Colombo 5790, Bloco G-90, Maringá, PR, CEP 87020-900, Brazil
A.J. Prioli
Affiliation:
Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá, Av. Colombo 5790, Bloco G-90, Maringá, PR, CEP 87020-900, Brazil

Abstract

Urocleidoides ectoparasites are mainly found on fish of the neotropical regions. Although molecular research on monogeneans is available, no genetic data exist characterizing species in the Urocleidoides genus. Some DNA sequences have been efficacious in systematic studies and in the reconstruction of phylogenies of fish parasites. Relevant roles have been given to the sequence of the mitochondrial gene of cytochrome c oxidase I (COI). This study characterized COI sequences of the parasites Urocleidoides malabaricusi and U. cuiabai in trahira fish Hoplias aff. malabaricus of the flood plain of the Upper River Paraná, Brazil. The two species under analysis were distinguished by sequencing and analysing a 420-bp fragment of the COI gene, which suggested the existence of the cryptic species U. malabaricusi.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beltran, S., Galinier, R., Allienne, J.F. & Boissier, J. (2008) Cheap, rapid and efficient DNA extraction method to perform multilocus microsatelite genotyping on all Schistosoma mansoni stages. Memórias do Instituto Oswaldo Cruz 103, 501503.Google Scholar
Bloch, M.E. (1794) Der malabarische hecht. pp. 149150 in Bloch, M.E. (Ed.) Naturgeschichte des Auslandische Fische. Berlin, Achter Theil. J. Moreno.Google Scholar
Cohen, S.C., Justo, M.C.N. & Kohn, A. (2013) South American Monogenoidea parasites of fishes, amphibians and reptiles. 663 pp. Rio de Janeiro, Oficina de Livros.Google Scholar
Criscione, C.D., Poulin, R. & Blouin, M.S. (2005) Molecular ecology of parasites: elucidating ecological and microevolutionary processes. Molecular Ecology 14, 22472257.Google Scholar
Eiras, J.C., Takemoto, R.M. & Pavanelli, G.C. (2010) Diversidade dos parasitos de peixes de água doce do Brasil. 333 pp. Maringá, Clichetec.Google Scholar
Gasques, L.S., Souza, G.T.R. & Graça, R.J. (2013) Das moléculas aos parasitos: a aplicação da biologia molecular nos estudos de Ictioparasitologia. pp. 149167 in Pavanelli, G.C., Takemoto, R.M. & Eiras, J.C. (Eds) Parasitologia, Peixes de água doce do Brasil. Maringá, Eduem.Google Scholar
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.Google Scholar
Hasegawa, M., Kishino, H. & Yano, T. (1985) Dating the human–ape split by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution 22, 160174.Google Scholar
Karling, L.C., Ueda, B.H., Takemoto, R.M. & Pavanelli, G.C. (2014) Scientometric study on the parasitology of freshwater fish in Brazil. Neotropical Helminthology 8, 131140.Google Scholar
Kritsky, D.C. & Thatcher, V.E. (1983) Neotropical Monogenea. V: Five new species from the Aruana, Osteoglossum bicirrosum Vandelli, a freshwater teleost from Brazil, with the proposal of Gonocleithrum n. gen. (Dactylogyridae: Ancyrocephalinae). Proceedings of the Biological Society of Washington 96, 581597.Google Scholar
Kritsky, D.C., Thatcher, V.E. & Boeger, W.A. (1986) Neotropical Monogenea. Revision of Urocleidoides (Dactylogyridae, Ancyrocephalinae). Proceedings of the Helminthological Society of Washington 53, 137.Google Scholar
Locke, S.A., Mclaughlin, J.D., Dayanandan, S. & Marcogliese, D.J. (2010) Diversity and specificity in Diplostomum spp. metacercariae in freshwater fishes revealed by cytochrome c oxidase I and internal transcribed spacer sequences. International Journal for Parasitology 40, 333343.Google Scholar
Mendoza-Palmero, C.A., Blasco-Costa, I. & Scholz, T. (2015) Molecular phylogeny of neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasites & Vectors 8, 164.Google Scholar
Mizelle, J.D. & Price, C.E. (1964) Studies on monogenetic trematodes. XXVII. Dactylogyrid species with the proposal of Urocleidoides gen. n. Journal of Parasitology 50, 579584.Google Scholar
Moreira, J., Scholz, T. & Luque, J.L. (2015) First data on the parasites of Hoplias aimara (Characiformes): description of two new species of gill monogeneans (Dactylogyridae). Acta Parasitologica 60, 254260.Google Scholar
Nadler, S.A. & De León, G.P.P. (2011) Integrating molecular and morphological approaches for characterizing parasite cryptic species: implications for parasitology. Parasitology 138, 16881709.Google Scholar
Perkins, E.M., Donnellan, S.C., Bertozzi, T. & Whittington, I.D. (2010) Closing the mitochondrial circle on paraphyly of the Monogenea (Platyhelminthes) infers evolution in the diet of parasitic flatworms. International Journal for Parasitology 40, 12371245.Google Scholar
Perkins, S.L., Martinsen, E.S. & Falk, B.G. (2011) Do molecules matter more than morphology? Promises and pitfalls in parasites. Parasitology 138, 16641674.Google Scholar
Plaisance, L., Rousset, V., Morand, S. & Littlewood, T.D.J. (2008) Colonization of Pacific islands by parasites of low dispersal ability: phylogeography of two monogenean species parasitizing butterflyfishes in the South Pacific Ocean. Journal of Biogeography 35, 6587.Google Scholar
Poisot, T., Verneau, O. & Desdevises, Y. (2011) Morphological and molecular evolution are not linked in Lamellodiscus (Platyhelminthes Monogenea). PLOS One 6, e26252.Google Scholar
Poulin, R. (2011) Uneven distribution of cryptic diversity among higher taxa of parasitic worms. Biology Letters 7, 241244.Google Scholar
Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes 7, 355364.Google Scholar
Rosim, D.F., Mendonza–Franco, E.F. & Luque, J.L. (2011) New and previously described species of Urocleidoides (Monogenoidea: Dactylogyridae) infecting the gills of Hoplias malabaricus (Characiformes: Erythrinidae) from Brazil. Journal of Parasitology 97, 406417.Google Scholar
Solé-Cava, A.M. (2008) Código de barras de DNA: o rabo que abana o cachorro. Ciência Hoje 41, 6567.Google Scholar
Takemoto, R.T., Luque, J.L., Bellay, S., Longhini, C.E. & Graça, R.J. (2013) Monogenea. pp. 273279 in Pavanelli, G.C., Takemoto, R.M. & Eiras, J.C. (Eds) Parasitologia, Peixes de água doce do Brasil. Maringá, Eduem.Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 27252729.Google Scholar
Thatcher, V.E. (2006) Aquatic biodiversity in Latin America: Amazon fish parasites. 2nd edn. Bulgaria, Pensoft.Google Scholar
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.Google Scholar
Vilas, R., Criscione, C.D. & Blouin, M.S. (2005) A comparison between mitochondrial DNA and the ribosomal internal transcribed regions in prospecting for cryptic species of platyhelminth parasites. Parasitology 121, 839846.Google Scholar