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Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis

Published online by Cambridge University Press:  05 June 2009

S. Misra ,
T. M. Mahopatra  and
S. Rathaur
S. Misra
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
T. M. Mahopatra
Affiliation:
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
S. Rathaur
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
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Abstract

Isozymic patterns of lactate dehydrogenase (E.C. 1.1.1.27) by polyacrylamide gel electrophoresis (PAGE) were observed m various categories of filariasis and controls, i.e. asymptomatic microfilaraemia and symptomatic amicrofilaraemia, endemic normal and non-endemic normal. Lactate dehydrogenase (LDH) activity was also observed amongst the above categories of patients. An increase in enzyme activity and change in the isozymic pattern was observed in the above categories of filaria infected serum. LDH activity doubled in asymptomatic microfilaraemia whereas in symptomatic amicrofilaraemia the increase in LDH activity was thirtyfold. The isozymic pattern of microfilaraemia cases showed the presence of three bands B4, A1B3, A2 B2, which are quite thick as compared to normal healthy subjects, whereas the patients with symptomatic amicrofilaraemia showed marked elevation of serum LDH-4 or A3B1. The LDH was partially purified by combined treatment of (NH4)2SO4 fractionation and gel filtration. The isozymic pattern of purified LDH showed a similar pattern.

Keywords

Wuchereria bancroftilactate dehydrogenasebiochemistryisoenzymesNematodafilariasis
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 142 - 146
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Davis, B. J. (1964) Disc electrophoresis II. Methods and application to human serum protein. Annals of the New York Academy of Sciences, 121, 404–427.Google Scholar
Horecker, H. L. & Kornberg, A. (1948) The Extinction coefficient of the reduced band of the pyridine nucleotide. Journal of Biological Chemistry, 175, 385–390.CrossRefGoogle Scholar
Kornberg, A., Colowick, S. P. & Kaplan, N. O. (1955) Lactate dehydrogenase in muscles. Methods in Enzymology. (editor Markert, C. L.), Vol. 1. pp. 441–445. Academic Press. New York.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the folin-phenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
Maizels, R. M., Denham, D. A. & Sutanto, I. (1985) Secreted and circulating antigen of the filarial parasite Brugia pahangi; Analysis of in Vitro released components and detection of parasite component in vivo. Molecular and Biochemical Parasitology, 17, 277–288.Google Scholar
Markert, B. E. (1975a) Isozymes: physiological function (editor Markert, C. L). Vol. 2. pp. 101–110, Academic Press, New York.Google Scholar
Markert, B. E. (1975b) Isozymes: physiological function (editor Markert, C. L.). Vol. 2. pp. 5–23. Academic Press, New York.Google Scholar
Markert, C. L. F. & Moller, ,(1959) Multiple forms of enzyme tissue ontogenic and sp. specific patterns. Proceedings of the National Academy of Sciences, USA. 45, 753–763.CrossRefGoogle Scholar
Markert, C. L. & Ursprung, H. (1962) The ontogeny of isozyme pattern of LDH in the mouse. The ontogeny of isozyme pattern of LDH in the mouse, 5, 363–381.Google Scholar
Ottesen, E. A. (1984) Immunological aspects of lymphatic filariasis and onchocereiasis in man. Immunological aspects of lymphatic filariasis and onchocereiasis in man, 78, 9–18.Google Scholar
Saxena, J. K., Bharadwaj, N., Rathaur, S. & Ghatak, S. (1986) Purification and characterisation of lactate dehydrogenase from Setaria cervi. Indian Journal of Medical Research, 84, 264–269.Google ScholarPubMed
Vessel, E. S. & Bearn, A. G. (1957) Localization of lactate dehydrogenase activity in serum fractions. Localization of lactate dehydrogenase activity in serum fractions, 94, 96–99.Google Scholar
Vessel, E. S. & Broddy, I. A. (1964) Biological application of lactate dehydrogenase isozymes. Biological application of lactate dehydrogenase isozymes, 121, 544–559.Google Scholar