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Induction of peripheral blood eosinophilia in mice by excretory and secretory antigen of Toxocara canis larvae

Published online by Cambridge University Press:  18 November 2009

Kazuo Sugane  and
Tomoo oshima
Kazuo Sugane
Affiliation:
Department of Parasitology, School of Medicine, Yokohama City University, Urafune-cho, Minami-ku 2-23, Yokohama city, Japan
Tomoo oshima
Affiliation:
Department of Parasitology, School of Medicine, Yokohama City University, Urafune-cho, Minami-ku 2-23, Yokohama city, Japan
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Abstract

Medium in which T. canis larvae had been cultured was applied to a Sephadex G-25 gel column and four fractions (Fr. 1-Fr. 4) were isolated. Each fraction was administered to mice by implanting a mini-osmotic pump into the peritoneal cavity and the subsequent peripheral blood eosinophilic counts were recorded. Mice given Fr. 1 showed a markedly greater eosinophilic response than did those given the other fractions. Complement activation by each fraction was investigated using normal human serum. Fr. 1 and Fr. 3 had a capacity to activate complement. The results suggest that Fr. 1 may be not only antigenetic and allergenic but also could induce eosinophilia and activate complement.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 2 , June 1984 , pp. 143 - 147
Copyright
Copyright © Cambridge University Press 1984

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