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In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants

Published online by Cambridge University Press:  05 June 2009

M. Larsen ,
J. Wolstrup ,
S. A. Henriksen ,
C. Dackman ,
J. Grønvold  and
P. Nansen
M. Larsen
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Wolstrup
Affiliation:
Department of Ecology and Molecular Biology, Section of Microbiology, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C., Denmark
S. A. Henriksen
Affiliation:
National Veterinary Laboratory, 27 Bülowsvej, DK-1870 Frederiksberg C., Denmark
C. Dackman
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Grønvold
Affiliation:
Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
P. Nansen
Affiliation:
Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
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Abstract

Laboratory experiments were designed to select nematophagous fungi that were able to survive in vitro conditions simulating passage through the gastro-intestinal tract of cattle. All of the tests were conducted at 39°C. In a primary stress selection step in diluted rumen fluid, 21 isolates were obtained. Each of the primary stress selected isolates was tested in synthetic saliva, rumen fluid simulating the activity in the rumen, rumen fluid followed by pepsin-hydrochloric acid treatment simulating the additional effect of ruminal and abomasal activity, pepsin-hydrochloric acid solution simulating conditions in the abomasum and finally in a trypsin solution as an example of enzyme activity in the gut. The effect of the rumen fluid alone, or rumen fluid followed by pepsin-hydrochloric acid treatment, were responsible for the reduction in surviving fungal isolates. Only six of thirteen isolates belonging to the genus Arthrobotrys survived while seven of eight isolates of the genus Duddingtonia survived. Fourteen isolates were tested for their predatory capacity in a dung pat bioassay. Fungi of the genera Arthrobotrys and Duddingtonia reduced the development of Ostertagia ostertagi third stage larvae by approximately 75% and 96% respectively compared to the number of larvae that developed from fungus-free control pats.

Keywords

nematophagous fungiArthrobotrysDuddingtoniastress selectionOstertagia ostertagibiological control
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 3 , September 1991 , pp. 193 - 200
Copyright
Copyright © Cambridge University Press 1991

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