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In vitro hatching of oncospheres of Taenia taeniaeformis using eggs isolated from fresh, frozen, formalin-fixed and ethanol-fixed segments

Published online by Cambridge University Press:  05 June 2009

T. Negita  and
A. Ito
T. Negita
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
A. Ito*
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
*
*Author for correspondence.
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Abstract

In order to establish a simple means of handling eggs of taeniid cestodes under non-biohazardous conditions, gravid segments of Taenia taeniaeformis were fixed in ethanol or formalin or were frozen. In vitro hatching of oncospheres was carried out by the 0.5% sodium hypochlorite method using eggs isolated from the nonviable and viable segments. No oncospheres hatched from formalized eggs, whereas almost all oncospheres hatched from all other eggs but the number of oncospheres recovered was highly variable. Oncospheres hatched from eggs isolated from frozen segments were highly fragile. The highest figures for in vitro hatching of oncospheres were recorded when eggs isolated either from segments fixed in 70% ethanol or from viable segments were used.

Type
Research Note
Information
Journal of Helminthology , Volume 68 , Issue 3 , September 1994 , pp. 271 - 272
Copyright
Copyright © Cambridge University Press 1994

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References

Ito, A. & Hashimoto, A. (1993) Vaccination with hatched but non-activated, non-viable oncospheres of Taenia taeniaeformis in rats. Journal of Helminthology 67, 165–168.Google Scholar
Ito, A., Fan, P.C., Chung, W.C. & Suzuki, M. (in press) Cross protection against Taenia taeniaeformis in rats vaccinated with Asian Taenia or T. saginata. Journal of Helminthology.Google Scholar
Lightowlers, M.W., Mitchell, G.F., Bowtell, D.D.L., Anders, R.F. & Rickard, M.D. (1984) Immunisation against Taenia taeniaeformis in mice: studies on the characterisation of antigens from oncospheres. International Journal for Parasitology 14, 321–333.Google Scholar
Lightowlers, M.W., Mitchell, G.F. & Rickard, M.D. (1993) Cestodes pp. 438–472 in Warren, K. S. (Ed.) Immunology and Molecular biology of parasite infections. Oxford, Blackwell.Google Scholar
Lightowlers, M.W. (in press) Anti-parasite vaccines: the coming of age. Veterinary Parasitology.Google Scholar