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In silico identification of tetraspanins in monopisthocotylean (Platyhelminthes: Monogenea) parasites of fish

Published online by Cambridge University Press:  24 March 2022

V. Caña-Bozada Open the ORCID record for V. Caña-Bozada [Opens in a new window] ,
F.N. Morales-Serna Open the ORCID record for F.N. Morales-Serna [Opens in a new window] ,
J.Á. Huerta-Ocampo Open the ORCID record for J.Á. Huerta-Ocampo [Opens in a new window]  and
A. Avalos-Soriano Open the ORCID record for A. Avalos-Soriano [Opens in a new window]
V. Caña-Bozada*
Affiliation:
Centro de Investigación en Alimentación y Desarrollo, A.C., Mazatlán82112, Sinaloa, Mexico
F.N. Morales-Serna
Affiliation:
Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Mazatlán82040, Sinaloa, Mexico
J.Á. Huerta-Ocampo
Affiliation:
Centro de Investigación en Alimentación y Desarrollo, Hermosillo83304, Sonora, Mexico Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico City, Mexico
A. Avalos-Soriano
Affiliation:
Centro de Investigación en Alimentación y Desarrollo, A.C., Mazatlán82112, Sinaloa, Mexico Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico City, Mexico
*
Author for correspondence: V. Caña-Bozada, E-mail: [email protected]
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Abstract

Tetraspanins are a superfamily of transmembrane proteins that in flatworms have structural roles in the development, maturation or stability of the tegument. Several tetraspanins are considered as potential candidates for vaccines or drugs against helminths. Monopisthocotylean monogeneans are ectoparasites of fish that are health hazards for farmed fish. The aim of this study was to identify in silico putative tetraspanins in the genomic datasets of four monopisthocotylean species. The analysis predicted and classified 40 tetraspanins in Rhabdosynochus viridisi, 39 in Scutogyrus longicornis, 22 in Gyrodactylus salaris and 13 in Neobenedenia melleni, belonging to 13 orthologous groups. The high divergence of tetraspanins made it difficult to annotate their function. However, a conserved group was identified in different metazoan taxa. According to this study, metazoan tetraspanins can be divided into 17 monophyletic groups. Of the 114 monogenean tetraspanins, only seven were phylogenetically close to tetraspanins from non-platyhelminth metazoans, which suggests that this group of proteins shows rapid sequence divergence. The similarity of the monopisthocotylean tetraspanins was highest with trematodes, followed by cestodes and then free-living platyhelminths. In total, 27 monopisthocotylean-specific and 34 flatworm-specific tetraspanins were identified. Four monogenean tetraspanins were orthologous to TSP-1, which is a candidate for the development of vaccines and a potential pharmacological target in trematodes and cestodes. Although studies of tetraspanins in parasitic flatworms are scarce, this is an interesting group of proteins for the development of new methods to control monogeneans.

Keywords

InvertebratesproteinsTSP-1bioinformaticsdrug targets
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e23
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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