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Immunofluorescent studies in filariasis: antibody levels in jirds (Meriones unguiculatus) infected with Brugia malayi

Published online by Cambridge University Press:  05 June 2009

Mulkit Singh ,
G. J. Kane ,
C. Burren  and
L. M. Mackinlay
Mulkit Singh
Affiliation:
Department of Microbiology, Faculty of Medicine, University of Singapore, Singapore 3
G. J. Kane
Affiliation:
Department of Microbiology, Wellcome Research Laboratories, Beckenham, Kent, England
C. Burren
Affiliation:
Department of Microbiology, Wellcome Research Laboratories, Beckenham, Kent, England
L. M. Mackinlay
Affiliation:
Department of Microbiology, Wellcome Research Laboratories, Beckenham, Kent, England
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Abstract

The indirect fluorescent antibody technique has been applied to detect antibody levels in jirds (meriones unguiculatus) infected with Brugia malayi. Sonicated antigens were prepared from microfilariae and adult worms. Sonicated microfilariae were found to be satisfactory for this purpose. Cyanogen bromide-activated sepharose coated with soluble antigens prepared from microfilariae and adult worms was also used to detect antibodies to Brugia infections. The present observation show that these techniques can be usefully applied for detection of filarial infections. Antibody titres in infected jirds generally ranged from 1: 16 –1:256 and were not affected by treatment with diethylcarbamazine.

Type
Research Papers
Information
Journal of Helminthology , Volume 54 , Issue 2 , June 1980 , pp. 147 - 153
Copyright
Copyright © Cambridge University Press 1980

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References

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