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Immunoblot analysis of serum IgG, IgA and IgE responses against larval excretory-secretory antigens of Anisakis simplex in patients with gastric anisakiasis

Published online by Cambridge University Press:  05 June 2009

Nobuaki Akao ,
Taka-Aki Ohyama  and
Kaoru Kondo
Nobuaki Akao
Affiliation:
Department of Parasitology, Faculty of Medicine, Kanazawa University, Kanazawa City 920, Japan
Taka-Aki Ohyama
Affiliation:
Department of Parasitology, Faculty of Medicine, Kanazawa University, Kanazawa City 920, Japan
Kaoru Kondo
Affiliation:
Department of Parasitology, Faculty of Medicine, Kanazawa University, Kanazawa City 920, Japan
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Abstract

To increase our understanding of the immune response to Anisakis infection, antigen specific IgG, IgA and IgE responses were identified using an immunoblot technique after polyacrylamide gel electrophoresis of excretory-secretory products from the larval stage of Anisakis simplex. Nine sera were drawn from proven cases of gastric anisakiasis within 3 days after symptoms had developed. The molecular weight of the major antigenic bands were distributed between 50 kDa and 120 kDa of the antigens. In nine cases of gastric anisakiasis, three of them were positive for IgG response, five for IgE, and six for IgA, respectively. None of control sera recognized the antigenic bands in IgA and IgE responses. In contrast, two controls had IgG antibodies against 1–2 proteins in the 65–95 kDa region. The antigenicity of the excretory–secretory products was lost following treatment by 0·2% trypsin, but not by 0·2 M periodic acid. Based on the results of reactivity to lectins, antigenic bands of the ES products possessed mucin type glycoconjugate residues in their protein portion. This indicates that the humoral responses of IgA and IgE antibodies to the larval ES antigens are a more reliable index of infection than that of the IgG response.

Keywords

Anisakis simplexES antigensIgAIgEimmune responseimmunoblotting
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 4 , December 1990 , pp. 310 - 318
Copyright
Copyright © Cambridge University Press 1990

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