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Immune responses induced by co-infection with Capillaria hepatica in Clonorchis sinensis-infected rats

Published online by Cambridge University Press:  08 August 2017

E.-K. Moon ,
S.-H. Lee ,
T.W. Goo  and
F.-S. Quan
E.-K. Moon
Affiliation:
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Korea
S.-H. Lee
Affiliation:
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea
T.W. Goo
Affiliation:
Department of Biochemistry, College of Medicine, Dongguk University, Gyeongju, Republic of Korea
F.-S. Quan*
Affiliation:
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Korea
*
*E-mail: [email protected]
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Abstract

Clonorchis sinensis and Capillaria hepatica are zoonotic parasites that mainly infect the liver and cause serious liver disorders. However, immunological parameters induced by co-infection with these parasites remain unknown. In this study, for the first time, we investigated immunological profiles induced by co-infection with C. hepatica (CH) in C. sinensis (CS)-infected rats (Sprague–Dawley). Rats were infected primarily with 50 metacercariae of C. sinensis; 4 weeks later, they were subsequently infected with 1000 infective C. hepatica eggs. Significantly higher levels of C. sinensis- or C. hepatica-specific IgG antibodies were found in the sera of rats. Interestingly, no cross-reacting antibody was observed between C. sinensis and C. hepatica infections. Significantly raised eosinophil levels were found in the blood of C. sinensis/C. hepatica co-infected rats (CS + CH) compared to the blood of rats infected singly with C. sinensis. Co-infected rats showed significantly higher levels of lymphocyte proliferation and cytokine production compared to a single C. sinensis infection. The worm burden of C. sinensis was significantly reduced in co-infected rats compared to the single C. sinensis infection. These results indicate that the eosinophils, lymphocyte proliferation and cytokine production induced by subsequent infection with C. hepatica in C. sinensis-infected rats might contribute to the observed C. sinensis worm reduction.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 4 , July 2018 , pp. 395 - 402
Copyright
Copyright © Cambridge University Press 2017 

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