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Genomic identification of Anisakis simplex isolates

Published online by Cambridge University Press:  05 June 2009

M. Siles ,
C. Cuéllar  and
M.J. Perteguer
M. Siles
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
C. Cuéllar*
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
M.J. Perteguer
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
*
†To whom correspondence should be addressed.
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Abstract

RAPD technique was used to differentiate individuals of Anisakis simplex obtained from Merluccius merluccius, Phycis blennoides, Conger conger and Lepidorhombus boscii, from the North Atlantic Ocean. The amplification patterns of the host DNA controls were markedly different from those obtained for the parasitic material. No variation within the same host was detected. The amplification patterns for larvae obtained from fish of the same genus were somewhat different. The amplification patterns of A. simplex isolates from M. merluccius, P. blennoides, C. conger and L. boscii, were different. These results suggest the possible existence of two populations with a considerable high genetic variability and a different adaptation to different host species.

Type
Research Note
Information
Journal of Helminthology , Volume 71 , Issue 1 , March 1997 , pp. 73 - 75
Copyright
Copyright © Cambridge University Press 1997

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References

Beverly-Burton, M., Nyman, O.L. & Pippy, H.C. (1977) The morphology and some observations on the population genetics of Anisakis simplex larvae (Nematoda: Ascaridata) from fishes of the North Atlantic. Journal of the Fisheries Research Board of Canada 34, 105–112.Google Scholar
Bullini, L., Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Berland, B. (1986) Genetic variation of ascaridoid worms with different life cycles. Evolution 40, 437–440.Google Scholar
Dias-Neto, E., Pereira, C., Rollinson, D., Katz, N., Pena, S.D.J. & Simpson, J.G. (1993) The random amplification of polymorphic DNA allows the identification of strains and species of Schistosoma. Molecular and Biochemical Parasitology 57, 83–88.Google Scholar
Ishikura, H., Kikuchi, K., Nagasawa, K., Ooiwa, T., Takamiya, H., Sato, N. & Sugane, K. (1993) Anisakis and anisakidosis. pp. 43–101in Sun, T. (Ed.) Progress in Clinical Parasitology, Vol. III. New York, Springer-Verlag.CrossRefGoogle Scholar
Nadler, S.A. (1990) Molecular approaches to studying helmith population genetics and phylogeny. International Journal for Parasitology 20, 11–29.CrossRefGoogle Scholar
Nascetti, G., Paggi, L., Orecchia, P., Smith, J.W., Mattiucci, S. & Bullini, L. (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and north-east Atlantic. International Journal for Parasitology 16, 97–104.CrossRefGoogle ScholarPubMed
Nascetti, G., Cianchi, R., Mattiucci, S., D'Amelio, S., Oreccia, P., Paggi, L., Bratley, J., Berland, B., Smith, J.W. & Bullini, L. (1993) Three sibling species within Contracaecum osculatum (Nematoda, Ascaridida, Ascaridoidea) from the Atlantic Artic-Boreal region: reproductive isolation and host preferences. International Journal for Parasitology 23, 105–120.Google Scholar
Orecchia, P., Paggi, L., Mattiucci, S., Smith, J.W., Nascetti, G. & Bullini, L. (1986) Electrophoretic identification of larvae and adults of Anisakis (Ascaridida: Anisakidae). Journal of Helminthology 60, 331–339.CrossRefGoogle ScholarPubMed
Siles, M., Cuesta, C. & César, M. (1993) Random amplified polymorphic DNA technique for speciation studies of Echinococcus granulosus. Parasitology Research 76, 343–345.Google Scholar
Sugane, K., Liu, Q. & Matsuura, T. (1989) Restriction fragment length polymorphisms of Anisakidae larvae. Journal of Helminthology 63, 269–274.CrossRefGoogle Scholar
Welsh, J. & McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213–7218.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1991) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.Google Scholar