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First record of the anisakid nematode Anisakis nascettii in the Gervais' beaked whale Mesoplodon europaeus from Brazil

Published online by Cambridge University Press:  28 October 2014

M.I.N. Di Azevedo ,
V.L. Carvalho  and
A.M. Iñiguez
M.I.N. Di Azevedo
Affiliation:
LABTRIP, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, 21045-900, Brazil
V.L. Carvalho
Affiliation:
Associação de Pesquisa e Preservação de Ecossistemas Aquáticos – AQUASIS, Caucaia, 61627-010, Ceará, Brazil
A.M. Iñiguez*
Affiliation:
LABTRIP, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, 21045-900, Brazil
*
* E-mail: [email protected]
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Abstract

Anisakid nematodes are parasites with an indirect life cycle, involving many hosts, including cetaceans that act as definitive hosts. Identification at species level is crucial for a better understanding of the epidemiology and ecology of these parasites, but an accurate diagnosis based only on morphology is difficult. In Brazil, genetic characterization of anisakids is still rare, with Anisakis typica and A. physeteris being the only two species identified by genetic markers until now. The definitive hosts of A. nascettii are Mesoplodon grayi, M. bowdoini, M. layardii and M. mirus. Geographical locations of A. nascettii include the coasts of New Zealand, South Africa and Europe. In this study, a nematode was collected from a Gervais' beaked whale, Mesoplodon europaeus, stranded in Ceará State, north-east Brazil. Genetic analysis based on 18S rDNA, internal transcribed spacer (ITS) region and mtDNA cox2 gene revealed 100% identity with Anisakis sp. U94365, 99% with Anisakis sp. (MP-2005 EU718477 A. nascettii) and 99% with A. nascettii DQ116431, respectively. Phylogenetic analyses of ITS and cox2 sequences using both neighbour-joining and maximum-likelihood methods provided strong support for a clade with only A. nascettii specimens. This study demonstrated for the first time the identification of A. nascettii in the Brazilian Atlantic Coast, revealing a wider geographical distribution of this parasite worldwide and extending the range of its definitive hosts to a new Mesoplodon species, M. europaeus, the Gervais' beaked whale.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 48 - 53
Copyright
Copyright © Cambridge University Press 2014 

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References

Audicana, M.T., Ansotegui, I.J., de Corres, L.F. & Kennedy, M.W. (2002) Anisakis simplex: dangerous – dead and alive? Trends in Parasitology 18, 20–25.CrossRefGoogle ScholarPubMed
Borges, J.N., Cunha, L.F., Santos, H.L., Monteiro-Neto, C. & Santos, C.P. (2012) Morphological and molecular diagnosis of anisakid nematode larvae from cutlassfish (Trichiurus lepturus) off the coast of Rio de Janeiro, Brazil. PLoS One 7, e40447.CrossRefGoogle ScholarPubMed
Carvalho, V.L., Bevilaqua, C.M., Iniguez, A.M., Mathews-Cascon, H., Ribeiro, F.B., Pessoa, L.M., de Meirelles, A.C., Borges, J.C., Marigo, J., Soares, L. & de Lima Silva, F.J. (2010) Metazoan parasites of cetaceans off the northeastern coast of Brazil. Veterinary Parasitology 173, 116–122.CrossRefGoogle ScholarPubMed
Cavallero, S., Nadler, S.A., Paggi, L., Barros, N.B. & D'Amelio, S. (2011) Molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern Atlantic coasts of USA, Gulf of Mexico, and Caribbean Sea. Parasitology Research 108, 781–792.CrossRefGoogle ScholarPubMed
Culik, B.M. 2010 Odontocetes – the toothed whales. Distribution, behaviour, migration and threats. Maps by IUCN. Bonn, Germany, UNEP/CMS Secretariat. Available athttp://www.cms.int/reports/small_cetaceans/index.htm (accessed accessed 15 September 2012).Google Scholar
D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanco, M. & Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223–226.CrossRefGoogle ScholarPubMed
Davey, J.T. (1971) A revision of the genus Anisakis Dujardin, 1845 (Nematoda: Ascaridata). Journal of Helminthology 45, 51–72.CrossRefGoogle Scholar
Dorny, P., Praet, N., Deckers, N. & Gabriel, S. (2009) Emerging food-borne parasites. Veterinary Parasitology 163, 196–206.CrossRefGoogle ScholarPubMed
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41, 95–98.Google Scholar
Iglesias, R., D'Amelio, S., Ingrosso, S., Farjallah, S., Martinez-Cedeira, J.A. & Garcia-Estevez, J.M. (2008) Molecular and morphological evidence for the occurrence of Anisakis sp. A (Nematoda, Anisakidae) in the Blainville's beaked whale Mesoplodon densirostris. Journal of Helminthology 82, 305–308.CrossRefGoogle Scholar
Iñiguez, A.M., Santos, C.P. & Vicente, A.C. (2009) Genetic characterization of Anisakis typica and Anisakis physeteris from marine mammals and fish from the Atlantic Ocean off Brazil. Veterinary Parasitology 165, 350–356.CrossRefGoogle ScholarPubMed
Iñiguez, A.M., Carvalho, V.L., Motta, M.R., Pinheiro, D.C. & Vicente, A.C. (2011) Genetic analysis of Anisakis typica (Nematoda: Anisakidae) from cetaceans of the northeast coast of Brazil: new data on its definitive hosts. Veterinary Parasitology 178, 293–299.CrossRefGoogle ScholarPubMed
Knoff, M., Felizardo, N.N., Iñiguez, A.M., Maldonado, A. Jr, Torres, E.J.L., Pinto, R.M. & Gomes, D.C. (2012) Genetic and morphological characterization of a new species of the genus Hysterothylacium (Nematoda) from Paralichthys isosceles Jordan, 1890 (Pisces: Teleostei) of the neotropical region, State of Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz 107, 186–193.CrossRefGoogle ScholarPubMed
Luque, J.L., Muniz-Pereira, L.C., Siciliano, S., Siqueira, L.R., Oliveira, M.S. & Vieira, F.M. (2010) Checklist of helminth parasites of cetaceans of Brazil. Zootaxa 2548, 57–68.CrossRefGoogle Scholar
Martins, A.M.A., Alves-Junior, T.T., Furtado-Neto, M.A.A. & Lien, J. (2004) The most northern record of Gervais' beaked whale, Mesoplodon europaeus (Gervais, 1855), for the southern hemisphere. Latin American Journal of Aquatic Mammals 3, 151–155.CrossRefGoogle Scholar
Mattiucci, S. & Nascetti, G. (2006) Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: an update. Parasite 13, 99–113.CrossRefGoogle ScholarPubMed
Mattiucci, S. & Nascetti, G. (2008) Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host–parasite co-evolutionary processes. Advances in Parasitology 66, 47–148.CrossRefGoogle ScholarPubMed
Mattiucci, S., Paggi, L., Nascetti, G., Santos, C.P., Costa, G., Di Beneditto, A.P., Ramos, R., Argyrou, M., Cianchi, R. & Bullini, L. (2002) Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae). Systematic Parasitology 51, 159–170.CrossRefGoogle Scholar
Mattiucci, S., Abaunza, P., Damiano, S., Garcia, A., Santos, M.N. & Nascetti, G. (2007) Distribution of Anisakis larvae identified by genetic markers, and their use for stock characterization of demersal and pelagic fish from European waters: an update. Journal of Helminthology 81, 117–127.CrossRefGoogle ScholarPubMed
Mattiucci, S., Paoletti, M. & Webb, S.C. (2009) Anisakis nascettii n. sp. (Nematoda: Anisakidae) from beaked whales of the southern hemisphere: morphological description, genetic relationships between congeners and ecological data. Systematic Parasitology 74, 199–217.CrossRefGoogle Scholar
Mattiucci, S., Cipriani, P., Webb, S.C., Paoletti, M., Marcer, F., Bellisario, B., Gibson, D.I. & Nascetti, G. (2014) Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). Journal of Parasitology 100, 199–214.CrossRefGoogle Scholar
Nadler, S.A. & Hudspeth, D.S. (2000) Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution. Journal of Parasitology 86, 380–393.CrossRefGoogle ScholarPubMed
Pontes, T., D'Amelio, S., Costa, G. & Paggi, L. (2005) Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. Journal of Parasitology 91, 1430–1434.CrossRefGoogle ScholarPubMed
Santos, M.C., Zampirolli, E., Castro, A.F. & Alvarenga, F.S. (2003) A Gervais' beaked whale (Mesoplodon europaeus) washed ashore in southeastern Brazil: extra limital record? Aquatic Mamals 29, 404–410.CrossRefGoogle Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Thompson, J., Higgins, D. & Gibson, T. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.CrossRefGoogle ScholarPubMed
Valentini, A., Mattiucci, S., Bondanelli, P., Webb, S.C., Mignucci-Giannone, A.A., Colon-Llavina, M.M. & Nascetti, G. (2006) Genetic relationships among Anisakis species (Nematoda: Anisakidae) inferred from mitochondrial cox2 sequences, and comparison with allozyme data. Journal of Parasitology 92, 156–166.CrossRefGoogle ScholarPubMed