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First molecular identification of hydatid tapeworm Echinococcus granulosus sensu lato G6/G7 in Ecuador

Published online by Cambridge University Press:  04 November 2019

D. Ramos-Sarmiento Open the ORCID record for D. Ramos-Sarmiento [Opens in a new window]  and
V. Chiluisa-Utreras Open the ORCID record for V. Chiluisa-Utreras [Opens in a new window]
D. Ramos-Sarmiento*
Affiliation:
Gestión de Investigación, Desarrollo e Innovación, Instituto Nacional de Investigación en Salud Pública, Iquique N14-285 y Yaguachi, Quito, Ecuador
V. Chiluisa-Utreras
Affiliation:
Grupo de Investigación BIOARN, Universidad Politécnica Salesiana, 12 de octubre 2422 y Wilson, Quito, Ecuador
*
Author for correspondence: D. Ramos-Sarmiento, E-mail: [email protected]
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Abstract

Echinococcosis is a zoonotic parasitic illness that can cause significant disabilities, and even death for sick people. The disease is caused by the larval stage of cestodes belonging to the Echinococcus genus. In this study, multiple hydatid cysts were excised from an infected porcine liver. The identification of the parasitic species was made by the morphometric assessment of rostellar hooks and molecular detection of ribosomal DNA extant in protoscoleces of the hydatid sand. Rostellar hooks presented an average length of 27.4 µm by optical microscopy. Parasite DNA were detected in samples of hydatid sediment and positive controls by polymerase chain reaction. In conclusion, Echinococcus granulosus was recognized in samples of porcine hydatid cysts by microscopic observation, and the E. granulosus sensu lato strain E. canadensis G6/G7 was identified by molecular assay.

Keywords

DNA sequenceEcuadorgenus Echinococcushydatidrostellar hooks
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e100
Copyright
Copyright © Cambridge University Press 2019 

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References

Agudelo-Higuita, NI, Brunetti, E and McCloskey, C (2016) Cystic Echinococcosis. Journal of Clinical Microbiology 54, 518–523.Google Scholar
Álvarez-Rojas, CA (2016) Epidemiología molecular de Echinococcus granulosus en las Américas: implicaciones para el control y diagnóstico. Revista Parasitología Latinoamericana 65, 5–19.Google Scholar
Álvarez-Rojas, CA, Romig, T and Lightowlers, MW (2014) Echinococcus granulosus sensu lato genotypes infecting humans–review of current knowledge. International Journal for Parasitology 44, 9–18.Google Scholar
Berger, S (2019) Infectious diseases of Ecuador. 2019 edn. 346 pp. Los Angeles, GIDEON Informatics Inc.Google Scholar
Calvopiña, M, Cartagena, H, López, C, Guerrero, R, Amunarriz, M, Guderian, R and Guevara, A (1993) Hidatidosis en el Ecuador: informe del undecimoundécimo caso Echinococcus vogeli. Revista Científica Actualidad 17, 19–22.Google Scholar
Centers for Disease Control and Prevention, CDC (2002) Echinococcosis biology. Available at https://www.cdc.gov/parasites/echinococcosis/biology.html (accessed 5 October 2018).Google Scholar
Cucher, MA, Macchiaroli, N, Baldi, G, et al. (2016) Cystic echinococcosis in South America: systematic review of species and genotypes of Echinococcus granulosus sensu lato in humans and natural domestic hosts. Tropical Medicine and International Health 21, 166–175.Google Scholar
D'Alessandro, A and Rausch, RL (2008) New aspects of neotropical polycystic (Echinococcus vogeli) and unicystic (Echinococcus oligarthrus) echinococcosis. Clinical Microbiology Reviews 21, 380–401.Google Scholar
D'Alessandro, A, Rausch, RL, Cuello, C and Aristizábal, N (1978) Echinococcosis vogeli in man with a review of polycystic hydatid disease in Colombia and neighboring countries. The American Journal of Tropical Medicine and Hygiene 28, 303–317.Google Scholar
Eckert, J, Gemmell, MA, Meslin, FX and Pawlowski, ZS and World Health Organization (2001) WHOI/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. 1st edn. 265 pp. Paris, World Organization for Animal Health.Google Scholar
Girard de Kaminsky, R (2003) Métodos para laboratorios de atención primaria de salud. 2nd edn. 188 pp. Tegucigalpa, Universidad Nacional Autónoma de Honduras y Hospital-Escuela.Google Scholar
Instituto Nacional de Estadísticas y Censos, INEC (2017) Registro estadístico de camas y egresos hospitalarios. Available at http://www.ecuadorencifras.gob.ec/camas-y-egresos-hospitalarios/ (accessed 13 November 2018).Google Scholar
Martínez, P, Cáceres, D and Canals, M (2016) Hidatidosis: un problema no resuelto en Chile. Revista Parasitología Latinoamericana 65, 20–29.Google Scholar
Nakao, M, Lavikainen, A, Yanagida, T and Ito, A (2013) Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). International Journal for Parasitology 43, 1017–1029.Google Scholar
Pavletic, CF, Larrieu, E, Guarnera, EA, et al. (2017) Cystic echinococcosis in South America: a call for action. Pan American Journal of Public Health 41, e42.Google Scholar
Roming, T, Ebi, D and Wassermann, M (2015) Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Veterinary Parasitology 213, 76–84.Google Scholar
Rosales, S, Gavidia, C, Lopera, L, Barrón, E, Ninaquispe, B, Calderón, C and Gonzáles, A (2008) Obtención de Echinococcus granulosus en caninos infectados experimentalmente con protoescólices de quistes hidatídicos. Revista de Investigaciones Veterinarias del Perú 19, 37–42.Google Scholar
Scott, JC, Stefaniak, J, Pawlowski, ZS and McManus, DP (1997) Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitology 114, 37–43.Google Scholar
Smyth, JD and Davies, Z (1974) ln vitro culture of protoscoleces from sheep. International Journal for Parasitology 4, 443–445.Google Scholar
Vizcaychipi, KA, Sosa, S, Camicia, F, Santillán, G, Casalins, M and Nigro, MC (2012) Hidatidosis retroperitoneal secundaria a quiste hidatídico de localización hepática. Revista Argentina de Microbiología 44, 278–282.Google Scholar
World Health Organization (WHO) (2018) Neglected tropical diseases. New approach needed to tackle parasitic liver disease in Europe and Turkey. Available at https://www.who.int/neglected_diseases/news/new-approach-needeed-to-tackle-echinococcosis-europe/en/ (accessed 1 September 2019).Google Scholar