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Fate of developing larvae of Onchocerca lienalis and O.volvulus in micropore chambers implanted into laboratory hosts

Published online by Cambridge University Press:  05 June 2009

A. E. Bianco ,
M. B. Mustafa  and
P. J. Ham
A. E. Bianco
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
M. B. Mustafa
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
P. J. Ham
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
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Abstract

Infective larvae of Onchocerca lienalis and O.volvulus implanted subcutaneously within micropore chambers into laboratory hosts moulted to the fourth stage (L4) and underwent limited development and growth. Similar recoveries of O. lienalis L4 larvae in the range of 33–66% were obtained from chambers implanted into CBA and BALB/c strains of mice, jirds, and the natural bovine host. A relatively constant proportion of larvae survived up to 24 days post implantation and thereafter recoveries declined, although some worms were still alive after 96 days. Recoveries of O. volvulus L4 larvae from chambers given to normal or T-cell deprived mice were equivalent to one another and to those obtained with O. lienalis. Moulting of O. lienalis in chambers was observed on days 3 and 5, in close accordance with the timing of the third moult in cattle following systemic infection. Moulting of O. volvulus occurred between days 3–6. Morphological changes in developing larvae included a small but significant increase in length, a transient increase in width, and early development of the spicular primordia and genital tube. L4 larvae of O. lienalis, but not those of O. volvulus, exhibited 3 distinct caudal papillae not present on infective larvae.

Keywords

Onchocerca lienalisOnchocerca volvulusmouseMeriones unguiculatussubcutaneous implantationdevelopment
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 3 , September 1989 , pp. 218 - 226
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Abraham, D., Weiner, J. & Farrell, J. P. (1986) Protective immune responses of the jird to larval Dipetalonema viteae. Immunology, 57, 165–169.Google Scholar
Abraham, D., Grieve, R. B., Mika-Grieve, M. & Seibert, B. P. (1988) Active and passive immunisation of mice against larval Dirofilaria immitis. Journal of Parasitology, 74, 275–282.Google Scholar
Aoki, Y., Vincent, A. L., Ash, L. R. & Katamine, D. (1980) Scanning electron microscopy of third- and fourth-stage larvae and adults of Brugia pahangi (Nematoda:Filarioidea). Journal of Parasitology, 66, 449–457.Google Scholar
Bianco, A. E., Ham, P., El Sinnary, K. & Nelson, G. S. (1980) Large-scale recovery of Onchocerca microfilariae from naturally infected cattle and horses. Transactions of the Royal Society of Tropical Medicine and Hygiene, 74, 109.Google Scholar
Bianco, A. E., Ham, P. J., Townson, S., Mustafa, M. B. & Nelson, G. S. (1989) A semi-automated system of intrathoracic injection for the large-scale production of Onchocerca lienalis infective larvae. Tropical Medicine and Parasitology, 40, 57–64.Google Scholar
Bianco, A. E. & Muller, R. (1982) Experimental transmission of Onchocerca lienalis to calves. In: Parasites – Their World and Ours. Proceedings of the Fifth International Congress of Parasitology, August 7–14, Toronto, 1982. Molecular and Biochemical Parasitology, Supplement p. 349.Google Scholar
Carlow, C. K. S., Muller, R. & Bianco, A. E. (1986) Further studies on the resistance to Onchocerca microfilariae in CBA mice. Tropical Medicine and Parasitology, 37, 276–281.Google Scholar
Chandrashekar, R., Rao, U. R. & Subrahmanyam, D. (1985) Serum dependent cell-mediated immune reactions to Brugia pahangi infective larvae. Parasite Immunology, 7, 633–641.Google Scholar
Court, J. P. (1982) A diffusion chamber technique for detecting compounds with prophylactic activity against Brugia pahangi. Tropenmedizin und Parasitologie, 33, 83–86.Google ScholarPubMed
Delves, C. J. & Howells, R. E. (1985) Development of Dirofilaria immitis third-stage larvae (Nematoda:Filarioidea) in micropore chambers implanted into surrogate hosts. Tropenmedizin und Parasitologie, 36, 29–31.Google Scholar
Doenhoff, M. J., Musallam, R., Bain, J. & McGregor, A. (1979) Schistosoma mansoni infections in T-cell deprived mice, and the ameliorating effect of administering homologous chronic infection serum. I. Pathogenesis. American Society of Tropical Medicine and Hygiene, 28, 260.CrossRefGoogle ScholarPubMed
Duke, B. O. L. (1962) Experimental transmission of Onchocerca volvulus from man to a chimpanzee. Transactions of the Royal Society of Tropical Medicine and Hygiene, 56, 271.Google Scholar
El Sheikh, H. & Ham, P. J. (1982) Human onchocerciasis: Cryopreservation of isolated microfilariae. Lancet, 1, 450.Google Scholar
El Sinnary, K. A., Bianco, A. E. & Williams, J. F. (1987) Implantation of adult Onchocerca gutturosa into laboratory rodents. Parasitology, 95, 155–158.Google Scholar
Franz, M. & Zielke, E. (1980) Scanning electron microscope study on larvae of Wuchereria bancrofti from the vector and from experimental rodent hosts. Tropenmedizin und Parasitologie, 31, 345–356.Google Scholar
Gass, R. F., Tanner, M. & Weiss, N. (1979) Development of Dipetalonema viteae third-stage larvae (Nematoda:Filarioidea) in micropore chambers implanted into jirds, hamsters, normal and immunised mice. Zeitschrift für Parasitenkunde, 61, 73–82.Google Scholar
Ham, P. J. & Bianco, A. E. (1983) Development of Onchocerca volvulus from cryopreserved microfilariae in three temperate species of laboratory-reared blackflies. Tropenmedizin und Parasitologie, 34, 137–139.Google Scholar
Kozek, W. J. & Figueroa, M. H. (1982) Attempts to establish Onchocerca volvulus infection in primates and small laboratory animals. Acta Tropica, 39, 317–324.Google ScholarPubMed
Lok, J. B., Pollack, R. J., Cupp, E. W., Bernardo, M. J., Donnelly, J. J. & Albiez, E.J. (1984) Development of Onchocerca lienalis and O. volvulus from the third to fourth larval stage in vitro. Tropenmedizin und Parasitologie, 35, 209–211.Google Scholar
Muller, R. (1979) Identification of Onchocerca. In: Problems in the identification of parasites and their vectors (editors,Taylor, A. E. R., Muller, R.) Blackwell, Oxford, pp. 175–206.Google Scholar
Omar, M. S. & Schulz-key, H. (1978) Acid phosphatase activity in the larval stages of Onchocerca volvulus developing in the vector Simulium damnosum. Tropenmedizin und Parasitologie, 29, 359–363.Google ScholarPubMed
Raybould, J. N. (1981) Present progress towards the laboratory colonisation of members of the Simulium damnosum complex. In: Blackflies. The future for biological methods of integrated control. (editor, Laird, M.) Academic Press, New York. pp. 307–315Google Scholar
Strote, G. (1985) Development of infective larvae of Onchocerca volvulus in diffusion chambers implanted into Mastomys natalensis. Tropenmedizin und Parasitologie, 36, 120–122.Google Scholar
Suswillo, R. R., Nelson, G. S., Muller, R., McGreevy, P. B., Duke, B. O. L. & Denham, D. A. (1977) Attempts to infect jirds (Meriones unguiculatus) with Wuchereria bancrofti, Onchocerca volvulus, Loa loa and Mansonella ozzardi. Journal of Helminthology, 46, 132–134.Google Scholar
Townson, S. & Bianco, A. E. (1982) Experimental infection of mice with the microfilariae of Onchocerca lienalis. Parasitology, 85, 283–293.Google Scholar
Townson, S., Bianco, A. E. & Owen, D. (1981a) Attempts to infect small laboratory animals with the infective larvae of Onchocerca lienalis. Journal of Helminthology, 55, 247–249.Google Scholar
Townson, S., El Sinnary, K. & Bianco, A. E. (1981b) Transplantation of Onchocerca into mice. Transactions of the Royal Society of Tropical Medicine and Hygiene, 75, 889.Google Scholar
Weiss, N. & Tanner, M. (1979) Studies on Dipetalonema viteae (Filarioidea). 3. Antibody dependent cell mediated destruction of microfilariae in vivo. Tropenmedizin und Parasitologie, 30, 73.Google Scholar