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Failure to demonstrate suppressor T cell activity in the lamina propria of Hymenolepis diminuta-infected rats

Published online by Cambridge University Press:  05 June 2009

C. Palmas ,
D. Wakelin ,
G. Bortoletti ,
A. R. Ecca  and
F. Gabriele
C. Palmas
Affiliation:
Istituto di Patologia Sperimentale, Sezione di Parassitologia, Università di Cagliari, Via della Pineta 77, 09125 Cagliari, Italy
D. Wakelin
Affiliation:
MRC Experimental Parasitology Group, Department of Zoology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
G. Bortoletti
Affiliation:
Istituto di Patologia Sperimentale, Sezione di Parassitologia, Università di Cagliari, Via della Pineta 77, 09125 Cagliari, Italy
A. R. Ecca
Affiliation:
Istituto di Patologia Sperimentale, Sezione di Parassitologia, Università di Cagliari, Via della Pineta 77, 09125 Cagliari, Italy
F. Gabriele
Affiliation:
Istituto di Patologia Sperimentale, Sezione di Parassitologia, Università di Cagliari, Via della Pineta 77, 09125 Cagliari, Italy
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Abstract

The aim of this study was to examine whether there was an increase of suppressor T cells, relative to helper T cells, in the intestinal lamina propria of Hymenolepis diminuta-infected rats, a condition which might allow the parasite to survive for the life-span of the host. Lamina propria cells were isolated by enzymatic procedure. All lymphocytes were passed over nylon wool columns in order to remove B cells; then the T cells were purified by a panning technique using monoclonal anti-T cell antibodies. Changes in the mitogen-stimulated synthesis of 1gM, IgG and IgA by normal peripheral blood indicator lymphocytes, as measured by sandwich ELISA, was used as an index of help and/or suppression. No significant suppressor T cell activity was observed in the cultures, either from control or infected intestine.

Keywords

ImmunoglobulinsIgAIgG1gMELISAlamina propriaT cellsB cellsCestodaratimmunosuppressionevasionHymenolepis diminuta, tumour
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 1 , March 1993 , pp. 17 - 23
Copyright
Copyright © Cambridge University Press 1993

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