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Experimental infection routes of Angiostrongylus cantonensis in mice

Published online by Cambridge University Press:  05 June 2009

Lian-Chen Wang ,
David Chao  and
Eng-Rin Chen
Lian-Chen Wang
Affiliation:
Department of Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, Republic of China Department of Parasitology, Kaohsiung Medical College, Kaohsiung 80424, Taiwan, Republic of China
David Chao*
Affiliation:
Department of Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, Republic of China
Eng-Rin Chen
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung 80424, Taiwan, Republic of China
*
‡Author for correspondence.
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Abstract

Stomach intubation is the most common method used in the experimental infection of animals with Angiostrongylus cantonensis. In order to compare the effectiveness of other possible transmission methods, groups of BALB/c mice were given infective third-stage larvae of A. cantonensis by different routes including intraperitoneal or subcutaneous injections, and penetration of anal mucosa, vaginal mucosa, conjunctival mucosa, lacerated skin, unabraded skin, foot pad and tail skin, while stomach intubation was used as control. Recovery of fifth-stage larvae was higher in mice inoculated with third-stage larvae subcutaneously. Successful infections were established through all experimental transmission routes except tail skin penetration. This study suggests that oral infection may not be the only route for the transmission of human angiostrongyliasis, and subcutaneous infection may be a better method for experimental infection.

Keywords

Angiostrongylus cantonensismouseinfection routeexperimental infection
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 4 , December 1991 , pp. 296 - 300
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

Alicata, J. E. & Brown, R. W. (1962) Observations on the method of human infection with Angiostrongylus cantonensis in Tahiti. Canadian Journal of Zoology, 40, 755–760.CrossRefGoogle Scholar
Alicata, J. E. (1965) Biology and distribution of the rat lungworm, Angiostrongylus cantonensis, and its relationship to eosinophilic meningoencephalitis and other neurological disorders of man and animals. Advances in Parasitology, 3, 223–248.Google Scholar
Araki, K., Uga, S. & Matsumura, T. (1986a) Penetration of third-stage larvae of Angiostrongylus cantonensis in vitro. I. Penetration apparatus and its application. Japanese Journal of Parasitology, 35, 89–93.Google Scholar
Araki, K., Uga, S. & Matsumura, T. (1986b) Penetration of third-stage larvae of Angiostrongylus cantonensis in vitro. II. Invasion to and passage through the rat stomach, duodenal, rectal walls and skin. Japanese Journal of Parasitology, 35, 389–94.Google Scholar
Bhaibulaya, M. (1975) Comparative studies on the life history of Angiostrongylus mackerrasae Bhaibulaya, 1968 and Angiostrongylus cantonensis (Chen, 1935). International Journal for Parasitology, 5, 7–20.CrossRefGoogle ScholarPubMed
Bhaibulaya, M. (1979) Geographical distribution of Angiostrongylus and angiostrongyliasis in Thailand, Indochina and Australia. In: Studies on Angiostrongyliasis in Eastern Asia and Australia, Special Publication No. 44 (editor, Cross, J. H.), pp. 49–52. U.S. Naval Medical Research Unit NAMRU 2: Taipei.Google Scholar
Carney, W. P. & Stafford, E. E. (1979) Angiostrongyliasis in Indonesia. In: Studies on Angiostrongyliasis in Eastern Asia and Australia, Special Publication No. 44 (editor, Cross, J. H.), pp. 14–25. U.S. Naval Medical Research Unit NAMRU 2: Taipei.Google Scholar
Chen, E. R. (1979) Angiostrongyliasis and eosinophilic meningitis on Taiwan. In: Studies on Angiostrongyliasis in Eastern Asia and Australia. Special Publication No. 44 (editor, Cross, J. H.), pp. 57–73. U.S. Naval Medical Research Unit NAMRU 2: Taipei.Google Scholar
Crook, J. K., Fulton, S. E. & Supanwong, K. (1971). The infectivity of third-stage Angiostrongylus cantonensis larvae shed from drowned Achatina fulica snails and the effect of chemical agents on infectivity. Transactions of the Royal Society of Tropical Medicine and Hygiene, 65, 602–605.Google Scholar
Garcia, E. G. (1979) Angiostrongylus cantonensis in Philippines. In: Studies on Angiostrongyliasis in Eastern Asia and Australia. Special Publication No. 44 (editor, Cross, J. H.), pp. 53–56. U.S. Naval Medical Research Unit NAMRU 2: Taipei.Google Scholar
Intermill, R. W., Palmer, C. P., Fredrick, R. M. & Tamashiro, H. (1972) Angiostrongylus cantonensis on Okinawa. Japanese Journal of Parasitology, 42, 355–359.Google ScholarPubMed
Jindrak, K. (1968) Early migration and pathogenicity of Angiostrongylus cantonensis in laboratory rats. Annals of Tropical Medicine and Parasitology, 62, 506–517.Google Scholar
Ko, R. C. (1981) Host-parasite relationship of Angiostrongylus cantonensis 2. Angiotropic behavior and abnormal site development. Zeitschrift für Parasitenkunde, 64, 195–202.Google Scholar
Malek, E. A. (1980) Nematodiases transmitted by snails: angiostrongyliasis. In: Snail-transmitted Parasitic Diseases, pp. 281–297. CRC Press: Florida.Google Scholar
Neff, H. (1971) Experimentelle Infektionen von Hunden mit Angiostrongylus vasorum (Nematoda). Dissertation. University of Zurich, Switzerland.Google Scholar
Otsuru, M. (1979) Angiostrongylus cantonensis and angiostrongyliasis in Japan. In: Studies on Angiostrongyliasis in Eastern Asia and Australia, Special Publication No. 44 (editor, Cross, J. H.), pp. 74–117. U.S. Naval Medical Research Unit NAMRU 2: Taipei.Google Scholar
Ubelaker, J. E., Caruso, J. & Pena, A. (1981) Experimental infection of Sigmodon hispidus with third-stage larvae of Angiostrongylus costaricensis. Journal of Parasitology, 67, 219–221.CrossRefGoogle ScholarPubMed
Waynforth, H. B. (1980) Routes and methods of administration. In: Experimental and Surgical Techniques in the Rat. pp. 17–61. Academic Press: London.Google Scholar
Weinstein, P. P., Rosen, L., Laquer, G. L. & Sawyer, T. K. (1963) Angiostrongylus cantonensis infections in rats and rhesus monkey, and observations on the survival of the parasite in vitro. American Journal of Tropical Medicine and Hygiene, 12, 358–377.CrossRefGoogle ScholarPubMed