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Evaluation of two heterologous recombinant antigens for the serological diagnosis of human polycystic echinococcosis

Published online by Cambridge University Press:  17 March 2022

D. Daipert-Garcia ,
V.G. Virginio ,
F.B. Oliveira ,
N.G. Siqueira ,
H.B. Ferreira  and
R. Rodrigues-Silva Open the ORCID record for R. Rodrigues-Silva [Opens in a new window]
D. Daipert-Garcia
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
V.G. Virginio
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
F.B. Oliveira
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
N.G. Siqueira
Affiliation:
Fundacão Hospital Estadual do Acre, Acre, Brazil Departamento de Medicina, Universidade Federal do Acre, Acre, Brazil
H.B. Ferreira
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
R. Rodrigues-Silva*
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
*
Author for correspondence: R. Rodrigues-Silva. E-mail: [email protected]
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Abstract

Polycystic echinococcosis (PE) is a zoonosis endemic in the Neotropical region of the Americas. It is caused by the larval stage of the cestode Echinococcus vogeli, which develops as harmful cysts that slowly grow in the liver, lungs and other organs of humans and other host species. Human PE diagnosis is usually based on clinical and epidemiological aspects and imaging techniques, often requiring confirmation by immunological assays. The currently available serological tests for detecting antibodies against Echinococcus spp. are mostly based on complex, variable and poorly characterized mixtures of native parasite antigens, which impairs specificity and/or sensitivity. In this scenario, the evaluation of well-characterized alternative antigens is urgently needed for the improvement of PE diagnosis. Here, two subunits (AgB8/1 and AgB8/2) of the major secretory antigen from Echinococcus granulosus (antigen B (AgB)), of diagnostic value for cystic echinococcosis, were validated for PE diagnosis. These antigens, produced as pure recombinant proteins (rAgB8/1 and rAgB8/2) in Escherichia coli, allowed detecting specific immunoglobulin G antibodies in sera from PE patients in an enzyme-linked immunosorbent assay, with sensitivities of 83.72% and 81.40%, respectively, and specificities of 83.12% and 80.09%, respectively. The use of recombinant proteins overcomes difficulties to obtain parasite material and reduced non-specific reactions and costs. Our results demonstrated reproducibility and accuracy high enough to be considered valid according to the acceptance criteria for Food and Drug Administration assay validation. This qualifies rAgB8/1 and rAgB8/2 as potential substitutes for the currently used parasite crude or partially purified antigens.

Keywords

Echinococcus vogelipolycystic echinococcosisantigen Brecombinant antigensELISAimmunodiagnosis
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e21
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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