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Evaluation of Annona muricata (Graviola) leaves activity against experimental trichinellosis: in vitro and in vivo studies

Published online by Cambridge University Press:  13 September 2021

E.S. El-Wakil Open the ORCID record for E.S. El-Wakil [Opens in a new window] ,
H.F. Abdelmaksoud ,
T.S. AbouShousha  and
M.M.I. Ghallab
E.S. El-Wakil*
Affiliation:
Department of Parasitology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt
H.F. Abdelmaksoud
Affiliation:
Department of Parasitology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt
T.S. AbouShousha
Affiliation:
Department of Pathology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt
M.M.I. Ghallab
Affiliation:
Department of Medical Parasitology, kafrelsheikh Faculty of Medicine, Kafrelsheikh University, ElGiesh street, kafrelsheikh governorate, 33516, Egypt
*
Author for correspondence: E.S. El-Wakil, E-mail: [email protected]
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Abstract

Our work aimed to evaluate the possible effect of Annona muricata (Graviola) leaf extract on Trichinella spiralis in in vitro and in vivo studies. Trichinella spiralis worms were isolated from infected mice and transferred to three culture media – group I (with no drugs), group II (contained Graviola) and group III (contained albendazole) – then they were examined using the electron microscope. In the in vivo study, mice were divided into five groups: GI (infected untreated), GII (prophylactically treated with Graviola for seven days before infection), GIII (infected and treated with Graviola), GIV (infected and treated with albendazole) and GV (infected and treated with a combination of Graviola plus albendazole in half doses). Drug effects were assessed by adults and larvae load beside the histopathological small intestinal and muscular changes. A significant reduction of adult and larval counts occurred in treated groups in comparison to the control group. Histopathologically, marked improvement in the small intestinal and muscular changes was observed in treated groups. Also, massive destruction of the cultured adults’ cuticle was detected in both drugs. This study revealed that Graviola leaves have potential activity against trichinellosis, especially in combination with albendazole, and could serve as an adjuvant to anti-trichinellosis drug therapy.

Keywords

Trichinella spiralisAnnona muricata (Graviola)prophylacticalbendazolemiceelectron microscopy
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e53
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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