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Evaluation of a double centrifugation technique for the detection of Anoplocephala eggs in horse faeces

Published online by Cambridge University Press:  08 December 2010

S. Rehbein ,
T. Lindner ,
M. Visser  and
R. Winter
S. Rehbein*
Affiliation:
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101Rohrdorf, Germany
T. Lindner
Affiliation:
IDT Biologika GmbH, Am Pharmapark, 06861Dessau-Roßlau, Germany
M. Visser
Affiliation:
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101Rohrdorf, Germany
R. Winter
Affiliation:
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101Rohrdorf, Germany
*
*Fax: +49-8032-707525 E-mail: [email protected]
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Abstract

Faecal samples of 250 horses from farms with a known history of tapeworm infection were examined comparatively for cestode eggs using a double centrifugation/combined sedimentation–floatation technique. From each faecal sample, three 5 g and three 15 g subsamples were processed, each using either saturated NaCl solution, specific gravity (sp. g.) 1.2 [NaCl]; concentrated sugar solution, sp. g. 1.26 [sugar]; or concentrated ZnSO4 solution, sp. g. 1.3 [ZnSO4] for floatation. In total, faeces from 187 horses ( = 74.8%) tested ‘positive’ for Anoplocephala eggs. Percentages of samples testing ‘positive’ for Anoplocephala ova were: 57.2% for 5 g faeces/NaCl, 66% for 15 g faeces/NaCl, 66% for 5 g faeces/sugar, 72.8% for 15 g faeces/sugar, 55.6% for 5 g faeces/ZnSO4, and 61.2% for 15 g faeces/ZnSO4, respectively. Processing of 15 g faecal samples resulted in a significant (P < 0.05; McNemar's χ2-test) increase in the percentage of Anoplocephala egg detection compared to processing of 5 g samples for all floatation solutions. By processing 15 g faecal samples using sugar solution for floatation, 97.3% of all samples that tested ‘positive’ for Anoplocephala eggs were identified; there was no significant difference between the rate of samples that tested ‘positive’ using 15 g faeces/sugar (72.8%) and the total rate of samples that tested ‘positive’ (74.8%). Conversely, percentages of ‘positive’ samples from other test combinations were significantly (P < 0.0001, McNemar's χ2-test) lower than the total rate of samples testing ‘positive’. Processing faecal samples using sugar solution for floatation gave significantly (P < 0.05, Wilcoxon test) higher Anoplocephala egg counts than using NaCl and ZnSO4 solutions, for both 5 g and 15 g faecal samples. The double centrifugation technique using 15 g faecal samples and concentrated sugar solution for floatation appeared to offer an advantage for the detection of Anoplocephala eggs in horse faeces compared to the other test combinations.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 4 , December 2011 , pp. 409 - 414
Copyright
Copyright © Cambridge University Press 2010

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References

Agneessens, J., Debever, P., Engelen, S. & Vercruysse, J. (1998) Prevalentie van Anoplocephala perfoliata bij paarden in België, en evaluatie van de diagnostische sedimentatie/flotatie techniek. Vlaams Diergeneeskundig Tijdschrift 67, 27–31.Google Scholar
Beelitz, P. & Gothe, R. (2001) Bandwurmbefall bei Schlachtpferden in Oberbayern: Befallshäufigkeit und –stärke sowie Korrelation zwischen Befall mit Adultwürmern und Einachweis im Enddarmkot. Pferdeheilkunde 17, 423–428.CrossRefGoogle Scholar
Beroza, G.A., Marcus, L.C., Williams, R. & Bauer, S.M. (1986) Laboratory diagnosis of Anoplocephala perfoliata infection in horses. Proceedings of the American Association of Equine Practicioners 32, 435–439.Google Scholar
Böhm, L.K. & Supperer, R. (1958) Beiträge zur Kenntnis tierischer Parasiten III. Zentralblatt für Bakteriologie, I. Abteilung, Originale 172, 298–309.Google Scholar
Boswinkel, M. & Sloet van Oldruitenborgh-Oosterbaan, M.M. (2007) Correlation between colic and antibody levels against Anoplocephala perfoliata in horses in the Netherlands. Tijdschrift voor Diergeneeskunde 132, 508–512.Google Scholar
Chlastáková, I., Vavrouchová, E., Kamler, M., Bodeček, Š. & Koudela, B. (2009) Comparison of coprological and molecular techniques for the diagnosis of Anoplocephala perfoliata infection of the horse. World Association for the Advancement of Veterinary Parasitology Congress. Calgary, Canada, 8–15 August, Abstracts, 161.Google Scholar
Çirak, V.Y., Hermosilla, C. & Bauer, C. (1996) Study on the gastrointestinal parasite fauna of ponies in northern Germany. Applied Parasitology 37, 239–244.Google Scholar
Collobert, C., Urbain, F., Tariel, G. & Lamidey, C. (1991) Le diagnostic coproscopique du téniasis des chevaux. Pratique Vétérinaire Équine 23, 41–44.Google Scholar
Drogemuller, M., Beelitz, P., Pfister, K., Schnieder, T. & Samson-Himmelstjerna, G.v. (2004) Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods. Veterinary Parasitology 124, 205–215.CrossRefGoogle ScholarPubMed
Drößigk, U. & Schuster, R. (1997) Bandwurmbefall bei Pferden – Probleme und Erfahrungen. Der Praktische Tierarzt 78, 564–567.Google Scholar
Epe, C., Behrens, T., Samson-Himmelstjerna, G.v. & Schnieder, T. (2001) Prävalenz von Bandwürmern bei Pferden (Anoplocephalidae) in Norddeutschland. Der Praktische Tierarzt 82, 37–42.Google Scholar
French, D.D., Chapman, M.R. & Klei, T.R. (1994) Effects of treatment with ivermectin for five years on the prevalence of Anoplocephala perfoliata in three Louisiana pony herds. Veterinary Record 135, 63–65.Google Scholar
Gasser, R.B., Williamson, R.M.C. & Beveridge, I. (2005) Anoplocephala perfoliata of horses – significant scope for further research, improved diagnosis and control. Parasitology 131, 1–13.CrossRefGoogle ScholarPubMed
Gundłach, J.L., Sadzikowski, A.B. & Tomczuk, K. (2003) Diagnostyka inwazji tasiemców u koni. Medycyna Weterynaryjna 59, 532–535.Google Scholar
Hasslinger, M.-A. & Tausend, S. (1989) Zur Bedeutung und Bekämpfung des Bandwurmbefalles beim Pferd. Der Praktische Tierarzt 79, 26–31.Google Scholar
Hearn, F.P.D. & Hearn, E.E. (1995) A simple diagnostic technique to better determine the prevalence of tapeworms. Journal of Equine Veterinary Science 15, 96–98.Google Scholar
Höglund, J., Ljungström, B.-L., Nilsson, O. & Uggla, A. (1995) Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Anoplocephala perfoliata in horse sera. Veterinary Parasitology 59, 97–106.Google Scholar
Höglund, J., Nilsson, O., Ljungström, B.-L., Hellander, J., Osterman Lind, E. & Uggla, A. (1998) Epidemiology of Anoplocephala perfoliata in foals on a stud farm in south-western Sweden. Veterinary Parasitology 75, 71–79.CrossRefGoogle ScholarPubMed
Ihler, C.F., Rootwelt, V., Heyeraas, A. & Dolvik, N.I. (1995) The prevalence and epidemiology of Anoplocephala perfoliata infection in Norway. Veterinary Research Communications 19, 487–494.Google Scholar
Kania, S.A. & Reinemeyer, C.R. (2005) Anoplocephala perfoliata coproantigen detection: a preliminary study. Veterinary Parasitology 127, 115–119.Google Scholar
Kjær, L.N., Lungholt, M.M., Nielsen, M.K., Olsen, S.N. & Maddox-Hyttel, C. (2007) Interpretation of serum antibody response to Anoplocephala perfoliata in relation to parasite burden and faecal egg count. Equine Veterinary Journal 39, 529–533.CrossRefGoogle ScholarPubMed
Maddox-Hyttel, C., Sørensen, T.S., Proudman, C.J., Farlam, J. & Andersen, B. (2005) Anoplocephala perfoliata infection i Danmark. Dansk Veterinærtidsskrift 88, 20–22.Google Scholar
Martins, I.V.F., Verocai, G.G., Melo, R.M.P.S., Freitas, I.F., Correia, T.R., Pereira, M.J.S. & Scott, F.B. (2003) Validação de uma modificação da técnica de centrífugo-flutuação (Beroza et al. 1986) para o diagóstico de cestóides em eqüídeos. Brazilian Journal of Veterinary Parasitology 12, 99–102.Google Scholar
Meana, A., Luzón, M., Corchero, J. & Gómez-Bautista, M. (1998) Reliability of coprological diagnosis of Anoplocephala perfoliata infection. Veterinary Parasitology 74, 79–83.CrossRefGoogle ScholarPubMed
Nilsson, O., Ljungstöm, B.-L., Höglund, J., Lundquist, H. & Uggla, A. (1995) Anoplocephala perfoliata in horses in Sweden: prevalence, infection levels and intestinal lesions. Acta Veterinaria Scandinavica 36, 319–328.CrossRefGoogle ScholarPubMed
Proudman, C.J. & Edwards, G.B. (1992) Validation of a centrifugation/flotation technique for the diagnosis of equine cestodiasis. Veterinary Record 131, 71–72.Google Scholar
Proudman, C.J. & Trees, A.J. (1996a) Correlation of antigen specific IgG and IgG(T) responses with Anoplocephala perfoliata infection intensity in the horse. Parasite Immunology 18, 499–506.Google Scholar
Proudman, C.J. & Trees, A.J. (1996b) Use of excretory/secretory antigens for the serodiagnosis of Anoplocephala perfoliata cestodiasis. Veterinary Parasitology 61, 239–247.Google Scholar
Rehbein, S., Visser, M. & Winter, R. (2000) Contribution to the knowledge of endoparasites of ponies in Germany.19. Tagung der Deutschen Gesellschaft für Parasitologie. Stuttgart-Hohenheim, Germany, 28 March–1 April, Abstracts, 109.Google Scholar
Rehbein, S., Visser, M. & Winter, R. (2002) Koproskopische Untersuchungen bei Pferden in Deutschland und Österreich. Pferdeheilkunde 18, 439–449.CrossRefGoogle Scholar
Rehbein, S., Visser, M., Winter, R. & Maidl, M. (2004) Untersuchungen zum Parasitenbefall bei 400 Schlachtpferden in Ostbayern. Tagung der Deutschen Gesellschaft für Veterinärmedizin, Fachgruppe Parasitologie und Parasitäre Krankheiten. Starnberg, Germany, 9–11 June, Abstracts, 2.Google Scholar
Sangioni, L.A., Vodotto, O., Luz Pereira, A.B. & Bonzi, G.L. (2000) Evaluation of the effectiveness of the method of centrifugation-fluctuation for the diagnosis of Anoplocephala perfoliata (Goeze, 1782) in equines. Brazilian Journal of Veterinary Parasitology 9, 51–54.Google Scholar
Skotarek, S.L. (2008) Epidemiology and diagnosis of Anoplocephala perfoliata in horses from Southern Alberta, Canada. MSc thesis, University of Lethbridge, Canada.Google Scholar
Slocombe, J.O.D. (1979) Prevalence and treatment of tapeworms in horses. Canadian Veterinary Journal 20, 136–140.Google ScholarPubMed
Slocombe, J.O.D. (2004) A modified critical test for the efficacy of pyrantel pamoate for Anoplocephala perfoliata in equids. Canadian Journal of Veterinary Science 68, 112–117.Google ScholarPubMed
Traversa, D., Fichi, G., Campigli, M., Rondolotti, A., Iorio, R., Proudman, C.J., Pellegrini, D. & Perrucci, S. (2008) A comparison of coprological, serological and molecular methods for the diagnosis of horse infection with Anoplocephala perfoliata (Cestoda, Cyclophyllidea). Veterinary Parasitology 152, 271–277.Google Scholar
Williamson, R.M.C., Beveridge, I. & Gasser, R.B. (1998) Coprological methods for the diagnosis of Anoplocephala perfoliata infection in the horse. Australian Veterinary Journal 76, 618–621.CrossRefGoogle ScholarPubMed