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The detection of antibodies in human and animal filariases by counterimmunioelectrophoresis with Dirofilaria immitis antigens

Published online by Cambridge University Press:  05 June 2009

Robert S. Desowitz  and
Steven R. Una
Robert S. Desowitz
Affiliation:
Department of Tropical Medicine and Medical Microbiology, School of Medicine, University of Hawaii 96816, USA
Steven R. Una
Affiliation:
Department of Tropical Medicine and Medical Microbiology, School of Medicine, University of Hawaii 96816, USA
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Abstract

Counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with Dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. Antigens used in the test were prepared from microfilariae and adult male D. immitis. Some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. The presence of soluble circulating antigen was detected in the sera of two dogs with high microfilaraemias.

Type
Research Article
Information
Journal of Helminthology , Volume 50 , Issue 1 , March 1976 , pp. 53 - 57
Copyright
Copyright © Cambridge University Press 1976

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References

REFERENCES

Biguet, J., d'Haussy, R., Aubry, M. and Rose, F. (1964) Etude des anticorps precipitants chez le sujets atteints d'Onchocercose. Bulletin de la Société de Pathologie exotique, 57, 1098–1116.Google Scholar
Biguet, J., d'Haussy, R., Capron, A., Tran Van Ky, and Aubry, M. (1962) Les antigènes d'Onchocerca volvulus. I. Etude immunoéletrophorétique préliminaire. Bulletin de la Société de Pathologie exotique, 55, 845–855.Google Scholar
Desowitz, R.S. and Berman, S.J. (1973) Epidemiological investigations on filariasis in the South Pacific and Indonesia. Unpublished consultants’ report to WHO.Google Scholar
Desowitz, R.S. and Hitchcock, J.C. (1974) Hyperendemic bancroftian filariasis in the Kingdom of Tonga: The application of the membrane filter concentration technique to an age-stratified blood survey. American Journal of Tropical Medicine and Hygiene, 23, 877–879.CrossRefGoogle Scholar
Desowitz, R.S. and Southgate, B.A. (1973) Studies on filariasis in the Pacific. 2. The persistence of microfilaraemia in diethylcarbamazine-treated populations of Fiji and Western Samoa. Diagnostic application of the membrane-filtration technique. Southeast Asian Journal of Tropical Medicine and Public Health, 4, 179–183.Google ScholarPubMed
Despommier, D., Muller, M., Jenks, B. and Fruitstove, M. (1974) Immunodiagnosis of human trichinosis using counterimmunoelectrophoresis and agar gel diffusion techniques. American Journal of Tropical Medicine and Hygiene, 23, 41–44.CrossRefGoogle Scholar
D'haussy, R., Capron, A., Rolland, A. and Biguet, J. (1972) Apport de la immunologie a l'epidemiologic de l'Onchocercose. Revue d'Immunologie, Paris, 36, 55–68.Google Scholar
Ellsworth, J.H. and Johnson, A.H. (1973) Precipitin test and soluble antigen flourcscent antibody technique for detecting Dirofilaria immitis in the dog. American Journal of Veterinary Research, 34, 682–692.Google Scholar
Gentilini, M., Pinon, J.M., Niel, G. and Rosin, G. (1973) Test de rosettes et d'inhibition de migration des leucocytes péripheriques dans les filarioses humaines. Bulletin de la Société de Pathologie exotique, 66, 133–139.Google Scholar
Kaeuffer, H., Merlin, M. and Laigret, J. (1974) Study of a brute antigenic extract of Dirofilaria immitis applied to the immunologie diagnosis of the lymphatic filariasis of Wuchereria bancrofti variety pacifica. Reference paper. Fourth Joint WHO/SPC Seminar on filariasis and vector control. Apia, Western Samoa, 1–10 July, 1974.Google Scholar
Krupp, I.M. (1974) Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis. American Journal of Tropical Medicine and Hygiene, 23, 27–30.CrossRefGoogle ScholarPubMed
Niel, G., Gidel, R., Couture, J., Pinon, J.M., Brengues, J. and Gentilini, M. (1972) Etude d'un extrait antigenique de la filaire Setaria labiato-papillosa en double diffusion et en immunoéléctrophoresé applique au diagnostic immunologiquc des filarioses. Medecine et Maladies infectieuses, 2, 193–202.CrossRefGoogle Scholar
Wheeling, C.H. and Hutchinson, W.F. (1971) Disc electrophoresis of soluble extracts of Dirofilaria immitis. Japanese Journal of experimental Medicine, 41, 171–176.Google ScholarPubMed