Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-27T02:31:08.008Z Has data issue: false hasContentIssue false
  • English
  • Français

Copro-PCR based detection of bovine schistosome infection in India

Published online by Cambridge University Press:  14 January 2015

B. Lakshmanan ,
K. Devada ,
S. Joseph ,
T.V. Aravindakshan  and
L. Sabu
B. Lakshmanan*
Affiliation:
Department of Veterinary Parasitology
K. Devada
Affiliation:
Department of Veterinary Parasitology
S. Joseph
Affiliation:
Department of Veterinary Microbiology
T.V. Aravindakshan
Affiliation:
Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala-680651, India
L. Sabu
Affiliation:
College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala-673576, India
*
* E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Schistosomosis and amphistomosis are the two economically important and widely prevalent snail-borne trematode infections in grazing cattle of southern India. Acute infections are symptomatically similar and difficult to detect by routine microscopy for eggs. The present study was directed towards the development of a copro-polymerase chain reaction (copro-PCR) for detection of bovine schistosome species, using custom-designed primers targeting 18S and 28S ribosomal RNA as well as mitochondrial DNA. The study demonstrated the enhanced diagnostic specificity of mitochondrial DNA markers over ribosomal RNA genes as genus-specific probes to detect schistosomes. We developed a sensitive PCR assay using primers designed from mitochondrial DNA sequences targeting the partial rrnl (16S rRNA), tCys (transfer RNA for cysteine) and partial rrnS (12S rRNA) genes of Schistosoma spindale to specifically detect schistosome infection from faecal samples of naturally infected bovines. The salient findings of the work also throw light on to the high similarity of the ribosomal RNA gene sequences of schistosomes with those of Gastrothylax crumenifer and Fischoederius elongatus, the most prevalent pouched amphistomes of the region. Further investigation has to be directed towards unravelling the complete gene sequences of 18S and 28S ribosomal RNA as well as mitochondrial DNA sequences of amphistome isolates from India.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 102 - 107
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aradaib, I.E., Omer, O.H., Abbas, B.B., Bushara, H.O., Elmalik, K.H., Saad, A.M., Osburn, B.I. & Taylor, M.G. (1995) Schistosoma bovis whole egg antigen did not protect zebu calves against experimental schistosomosis. Preventive Veterinary Medicine 21, 339–345.CrossRefGoogle Scholar
Barker, S.C. & Blair, D. (1996) Molecular phylogeny of Schistosoma species supports traditional groupings within the genus. Journal of Parasitology 82, 292–298.CrossRefGoogle ScholarPubMed
Chen, F., Li, J., Sugiyama, H., Weng, Y.B., Zou, F.C., Lin, R.Q., Yuan, Z.G., Song, H.Q., Zhu, X.Q. & Zhao, G.H. (2011) Comparative analysis of 18S and 28S rDNA sequences of Schistosoma japonicum from mainland China, the Philippines and Japan. Journal of Animal and Veterinary Advances 10, 2010–2015.Google Scholar
Dargie, J.D. (1980) The impact on production and mechanisms of pathogenesis of trematode infections in cattle and sheep. International Journal of Parasitology 17, 453–463.CrossRefGoogle Scholar
De Bont, J. & Vercruysse, J. (1997) The epidemiology and control of cattle schistosomiasis. Parasitology Today 13, 255–262.CrossRefGoogle ScholarPubMed
Doenhoff, M.J., Chiodini, P.L. & Hamilton, J.V. (2004) Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies? Trends in Parasitology 20, 35–39.CrossRefGoogle ScholarPubMed
Gobert, G.N., Chai, M., Duke, M. & McManus, D.P. (2005) Copro-PCR based detection of Schistosoma eggs using mitochondrial DNA markers. Molecular and Cellular Probes 19, 250–254.CrossRefGoogle ScholarPubMed
Gomes, L.I., Marques, L.H.S., Enk, M.J., Coelho, P.M.Z. & Rabello, A. (2009) Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Memorias do Insituto Oswaldo Cruz 104, 1194–1196.CrossRefGoogle ScholarPubMed
Gordon, C.A., Gray, D.J., Gobert, G.N. & McManus, D.P. (2011) DNA amplification approaches for the diagnosis of key parasitic helminth infections of human. Molecular and Cellular Probes 25, 143–152.CrossRefGoogle Scholar
Hamburger, J., Turetski, T., Kapeller, I. & Deresiewicz, R. (1991) Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species specific probe. Molecular Biochemical Parasitology 44, 73–80.CrossRefGoogle ScholarPubMed
Hamburger, J., Xin, H.X.Y., Ramsy, R.M., Jourdane, J. & Ruppel, A. (1998) A polymerase chain reaction assay for detecting snails infected with Bilharzia parasites (S. mansoni) from very early prepatency. American Journal of Tropical Medicine and Hygiene 59, 872–876.CrossRefGoogle ScholarPubMed
Hamburger, J., Hoffman, O., Kariuki, C.I., Muchiri, E., Ouma, J.H., Koech, D.K., Sturrock, R.F. & King, C.H. (2004) Large scale polymerase chain reaction based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. American Journal of Tropical Medicine and Hygiene 71, 765–773.CrossRefGoogle Scholar
Hendrix, C.M. & Robinson, C.V.T. (2006) Diagnostic parasitology for veterinary technicians. St. Louis, Missouri, Mosby Elsevier.Google Scholar
Johnston, D.A., Kane, R.A. & Rollinson, D. (1993) Small subunit (18S) ribosomal RNA gene divergence in the genus Schistosoma. Parasitology 107, 147–156.CrossRefGoogle ScholarPubMed
Kumar, V. (1999) Trematode infections and diseases of man and animals. Dordrecht, Kluwer Academic Publishers.CrossRefGoogle Scholar
Lakshmanan, B., Rauoof, A., Fawaz, M. & Subramanian, H. (2011) Abattoir survey of Schistosoma spindale infection in Thrissur. Journal of Veterinary and Animal Sciences 4, 53–54.Google Scholar
Littlewood, D.T.J., Lockyer, A.E., Webster, B.L., Johnston, D.A. & Le, T.H. (2006) The complete mitochondrial genomes of Schistosoma haematobium and Schistosoma spindale and the evolutionary history of mitochondrial genome changes among parasitic flatworms. Molecular Phylogenetics and Evolution 39, 452–467.CrossRefGoogle ScholarPubMed
Lockyer, A.E., Olson, P.D., Ostergaard, P., Rollinson, D., Johnston, D.A., Attwood, S.W., Southgate, V.R., Horak, P., Snyder, S.D., Le, T.H., Agatsuma, T., McManus, D.P., Carmichael, A.C., Naem, S. & Littlewood, D.T.J. (2003) The phylogeny of the Schistosomatidae based on three genes with emphasis on interrelationships of Schistosoma Weinland, 1858. Parasitology 126, 203–224.CrossRefGoogle ScholarPubMed
Mahfouz, A., Mahana, N., Rabee, I. & El-Amir, A. (2011) Evaluation of different immunological techniques for diagnosis of Schistosomiasis haematobium in Egypt. Life Science Journal 8, 858–867.Google Scholar
Marshall, I., Morrison, J.A. & Nyrinden, W. (1989) Comparison of potassium hydroxide and a modified Kato technique for semiquantitative estimation of Schistosoma mansoni in faeces. American Journal of Tropical Medicine and Hygiene 83, 31–35.Google Scholar
McCauley, E.H., Majid, A.A. & Tayeb, A. (1984) Economic evaluation of the production impact of bovine schistosomiasis and vaccination in Sudan. Preventive Veterinary Medicine 6, 735–754.CrossRefGoogle Scholar
Murthy, G.S.S., D'Souza, P.E. & Isloor, K.S. (2013) Evaluation of a polyclonal antibody based sandwich ELISA for the detection of faecal antigens in Schistosoma spindale infections in bovines. Journal of Parasite Diseases 37, 47–51.Google Scholar
Oliveira, L.M.A., Santos, H.L.C., Goncalves, M.M.L., Barreto, M.G.M. & Peralta, J.M. (2010) Evaluation of polymerase chain reaction as an additional tool for the diagnosis of low intensity Schistosoma mansoni infection. Diagnostic Microbiology and Infectious Disease 68, 416–421.CrossRefGoogle ScholarPubMed
Pardo, J., Carranza, C., Turrientes, M.C., Arellano, J.L.P., Velez, R.L., Ramajo, V. & Muro, A. (2004) Utility of Schistosoma bovis adult worm antigen for diagnosis of human schistosomiasis by enzyme linked immunosorbent assay and electro immuno transfer blot techniques. Clinical and Diagnostic Laboratory Immunology 11, 1165–1170.Google Scholar
Pontes, L.A., Dias-Neto, E. & Rabello, A. (2002) Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and faeces. American Journal of Tropical Medicine and Hygiene 66, 157–162.CrossRefGoogle Scholar
Ravindran, R., Lakshmanan, B., Ravishankar, C. & Subramanian, H. (2007) Visceral schistosomosis among domestic ruminants slaughtered in Wayanad, South India. South East Asian Journal of Tropical Medicine and Public Health 38, 1008–1010.Google ScholarPubMed
Sandoval, N., Siles-Lucas, M., Peres-Arellano, J.L., Carranza, C., Puente, S., Lopez-Aban, J. & Muro, A. (2006) A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples. Parasitology 133, 581–587.CrossRefGoogle ScholarPubMed
Singh, A., Singh, A. & Chaudhri, S.S. (2004) Visceral schistosomiasis of domestic animals in India: humoral immune status of infected sheep and goats against major polypeptide antigens of Schistosoma indicum and S. spindale. Parasite Immunology 26, 167–175.CrossRefGoogle ScholarPubMed
Sumanth, S., D'Souza, P.E. & Jagannath, M.S. (2003) Immunodiagnosis of nasal and visceral schistosomosis in cattle by Dot-ELISA. Indian Veterinary Journal 80, 495–498.Google Scholar
Sumanth, S., D'Souza, P.E. & Jagannath, M.S. (2004) A study of nasal and visceral schistosomosis in cattle slaughtered at an abattoir in Bangalore, South India. Revue Scientifique et Technique de l'office International des Epizooties 23, 937–942.CrossRefGoogle ScholarPubMed
Suzuki, T., Osada, Y., Kumagai, T., Hamada, A., Okuzawa, E. & Kanazawa, T. (2006) Early detection of Schistosoma mansoni infection by touch down PCR in a mouse model. Parasitology International 55, 213–218.CrossRefGoogle Scholar
Webster, B.L., Southgate, V.R. & Littlewood, D.T.J. (2006) A revision of the interrelationships of Schistosoma including the recently described Schistosoma guineensis. International Journal of Parasitology 36, 947–955.CrossRefGoogle ScholarPubMed
Webster, B.L., Rollinson, D., Stohard, J.R. & Littlewood, D.T.J. (2010) Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis. Journal of Helminthology 36, 947–955.Google Scholar