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The comparison of counterimmunoelectrophoresis with indirect haemagglutination test for detection of antibodies in experimentally infected guinea pigs with Toxocara canis*

Published online by Cambridge University Press:  05 June 2009

Mohammad S. Enayat
Affiliation:
Pathobiology Department, School of Public Health and Institute of Public Health Research, PO Box 1310, University of Tehran, Iran
Mohammad Pezeshki
Affiliation:
Pathobiology Department, School of Public Health and Institute of Public Health Research, PO Box 1310, University of Tehran, Iran

Abstract

The humoral response of guinea pigs infected with doses of 500, 1000, and 1500 infective Toxocara canis ova were measured by counterimmunoelectrophoresis and indirect haemmagglutination tests one to five weeks after infection. The antigens used in these measurements were prepared from adult T. canis. Counterimmunoelectrophoresis showed lower titres than haemagglutination method, but it was performed rapidly and with case. The possible use of these techniques for immunodiagnosis of human visceral larval migrans is discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1977

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References

REFERENCES

Aljeboori, T. I. and Ivey, M. H. (1970) An improved hemagglutination technique for detecting antibody against Toxocara canis. The American Journal of Tropical Medicine and Hygiene, 19, 244248.CrossRefGoogle ScholarPubMed
Baufine-Ducracq, H., Congineav, P. and Beauvais, B. (1973) Diagnostic par la reaction l'immunofluorescence du syndrome de larva migrans visceral. Bulletin de la Société de Pathologie Exotique, 66, 746751.Google Scholar
Beaver, P. C., Synder, C. H., Carrera, G. M., Dent, J. H. and Lafferty, J. W. (1952) Chronic eosinophilia due to visceral larva migrans. Report of three cases. Pediatrics, 55, 719.CrossRefGoogle Scholar
Bisseru, B. and Woodruff, A. W. (1968) The detection of circulating antibody in human Toxocara infections using the indirect flourescent antibody test. Journal of Clinical Pathology, 21, 449455.CrossRefGoogle Scholar
Collins, R. F. and Ivey, M. H. (1975) Specificity and sensitivity of skin test reactions to extracts of Toxocara canis and Ascaris suum. I. Skin tests done on infected guinea pigs. The American Journal of Tropical Medicine and Hygiene, 24, 455459.CrossRefGoogle ScholarPubMed
Desowitz, R. S. and Una, S. R. (1976) The detection of antibodies in human and animal filariasis by counterimmunoelectrophoresis with Dirofilaria immitis antigen. Journal of Helminthology, 50, 5357.CrossRefGoogle Scholar
Herbert, W. J. (1967) Passive haemagglutination. In Handbook of Experimental Immunology (ed Weir, D. M). pp. 720744Blackwell: Oxford.Google Scholar
Jung, R. C. and Pacheco, C. (1960) Use of a hemagglutination test in visceral larva migrans. The American Journal of Tropical Medicine and Hygiene, 9, 185191.CrossRefGoogle ScholarPubMed
Kagan, I. G., Norman, L. and Allain, D. S. (1959) Studies on the serology of visceral larva migrans. I. Hemagglutination and flocculation tests with purified Ascaris antigens. Journal of Immunology, 83, 297301.CrossRefGoogle ScholarPubMed
Kagan, I. G. (1968) Serological diagnosis of Visceral Larva Migrans. Clinical Pediatrics, 7, 508509.Google ScholarPubMed
Kagan, I. G. (1974) Advances in the immunodiagnosis of parasite infections. Zeitschrift für Parasitenkunde, 45, 163195.CrossRefGoogle Scholar
Krupp Iris, M. (1974a) Hemagglutination test for the detection of antibodies specific for Ascaris and Toxocara antigens in patients with suspected visceral larva migrans. The American Journal of Tropical Medicine and Hygiene, 23, 378384.CrossRefGoogle Scholar
Krupp Iris, M. (1974b) Comparison of counterimmunoelectrophoresis with other serological tests in the diagnosis of amebiasis. The American Journal of Tropical Medicine and Hygiene, 23, 2730.CrossRefGoogle Scholar
Viens, P., Strykawski, H., Richards, R. and Sonea, S. (1975) A modified immunofluorescent antibody technique for the serodiagnosis of human toxocaral migrans. Canadian Journal of Public Health, 66, 237240.Google ScholarPubMed