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Comparative evaluation of various immunodiagnostic tests for the diagnosis of Taenia solium cysticercosis in pigs, using fractionated antigens

Published online by Cambridge University Press:  05 June 2009

D. Kumar  and
S. N. S. Gaur
D. Kumar
Affiliation:
Department of Parasitology, College of Veterinary Sciences, G. B. Pant University of Agriculture & Technology, Pantnagar 263 145, Nainital, India
S. N. S. Gaur
Affiliation:
Department of Parasitology, College of Veterinary Sciences, G. B. Pant University of Agriculture & Technology, Pantnagar 263 145, Nainital, India
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Abstract

The sensitivity and specificity of double immunodiffusion (DID), indirect haemagglutination test (IHA), immunoelectrophoresis (IEP), counterimmunoelectrophoresis (CIEP) and enzyme-linked immunosorbent assay (ELISA) were evaluated and compared using saline extracted of Taenia solium larval scolex and its Sephadex G-200 fractionated 1st and 2nd peak as antigens. Various immunodiagnostic tests gave different results with different antigens. Highest sensitivity (92·5%) was obtained with ELISA using 1st peak antigen and lowest sensitivity was obtained with DID. However, 88·4% and 84·6% sensitivity was obtained with IHA and CIEP respectively using scolex antigen. CIEP gave better results as compared to IEP. Crude antigen gave high sensitivity but less specificity. It was concluded that CIEP can be used as a field test for the anti-mortem diagnosis and ELISA can be employed for laboratory confirmation of T. solium cysticercosis in pigs using frationated 1st peak antigen.

Keywords

Taenia soliumCysticercus cellulosaepigserodiagnosis
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 1 , March 1989 , pp. 13 - 17
Copyright
Copyright © Cambridge University Press 1989

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