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Characterization of glucose uptake by Trichuris globulosa (Nematoda) in vitro

Published online by Cambridge University Press:  05 June 2009

Nisha Jasra ,
Sankar N. Sanyal  and
S. Khera
Nisha Jasra
Affiliation:
Department of Zoology, Panjab University, Chandigarh-160014, India
Sankar N. Sanyal
Affiliation:
Biophysics, Panjab University, Chandigarh-160014, India
S. Khera
Affiliation:
Department of Zoology, Panjab University, Chandigarh-160014, India
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Abstract

14C-glucose uptake by adult Trichuris globulosa is found to be a non-linear function of time and limiting substrate concentration. The uptake is a two component process, an initial rapid burst is followed by a lower steady state, implying a mediated process. The uptake is dependent on Na+ ions which cannot be replaced by K+, Li+ or choline. The uptake is also dependent on pH, being maximal at pH 7·4. 14C-glucose absorption is markedly inhibited by glucose, phlorizin, ouabain and to a smaller extent by a number of monosaccharides and other sugar-phosphates, nucleosides and metabolic inhibitors like p-nitrophenyl phosphate and iodoacetate. The inhibition constant for glucose, phlorizin and ouabain has been found to be 8 mM, 5 mM and 7 mM, respectively. A modified Dixon-plot shows that glucose is a completely competitive inhibitor for 14C-glucose uptake while the nature of competitive inhibition of phlorizin and ouabain are found to be partial.

Keywords

Trichuris globulosaglucose uptakeinhibitors
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 3 , September 1990 , pp. 248 - 254
Copyright
Copyright © Cambridge University Press 1990

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