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Antibody responses in the wild vole, Clethrionomys rufocanus bedfordiae, naturally infected with Echinococcus multilocularis by Western blotting

Published online by Cambridge University Press:  05 June 2009

A. Ito ,
M. Nakao ,
M. Ito ,
T. Matsuzaki ,
M. Kamiya  and
H. Kutsumi
A. Ito
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
M. Ito
Affiliation:
Central Institute for Experimental Animals, Kawasaki 216, Japan
T. Matsuzaki
Affiliation:
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187, Japan
M. Kamiya
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Hokkaido University Sapporo 060, Japan
H. Kutsumi
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
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Abstract

Antibody responses against crude antigens and the two (Em18 and Em16) epitopes of Echinococcus mulfilocularis were analysed by Western blotting using sera from the wild vole, Clethrionomys rufocanus bedfordiae (Crb), which were captured in Hokkaido, Japan and found to have been naturally infected with eggs, and compared with those in patients of AHD and mice experimentally infected with protoscoleces of this parasite. Antibody responses in the wild vole were demonstrated most clearly with anti-Crb-IgG antiserum but more faintly with anti-rat-IgG antiserum and poorly with anti-mouse-IgG or anti-human-IgG antisera or Protein G. Although only two serum samples of the wild vole found naturally infected were analysed, antibody responses against Em18 and Em16 in these voles appeared to be similar to those in AHD patients but differed from those in mice.

Type
Research Note
Information
Journal of Helminthology , Volume 68 , Issue 3 , September 1994 , pp. 267 - 269
Copyright
Copyright © Cambridge University Press 1994

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