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Microarray expression profile of lncRNAs and mRNAs in the placenta of non-diabetic macrosomia

Published online by Cambridge University Press:  16 November 2017

G. Y. Song ,
Q. Na ,
D. Wang  and
C. Qiao
G. Y. Song
Affiliation:
Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China
Q. Na
Affiliation:
Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China
D. Wang
Affiliation:
Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China
C. Qiao*
Affiliation:
Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China
*
*Address for correspondence: C. Qiao, Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Shenyang, Liaoning 110004, P.R. China. (Email: [email protected])
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Abstract

Macrosomia, not only is closely associated with short-term, birth-related problems, but also has long-term consequences for the offspring. We investigated the expression of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in the placenta of macrosomia births using a microarray profile. The data showed that 2929 lncRNAs and 4574 mRNAs were upregulated in the placenta of macrosomia births compared with the normal birth weight group (fold change ⩾2.0, P<0.05), and 2127 lncRNAs and 2511 mRNAs were downregulated (fold change ⩾2.0, P<0.05). To detect the function of the differentially expressed lncRNAs and their possible relationship with the differentially expressed mRNAs, we also performed gene ontology analysis and pathway analysis. The results demonstrated that the PI3K-AKT signalling pathway, the mitogen-activated protein kinase (MAPK) signalling pathway, the focal adhesion pathway, the B cell receptor signalling pathway, and the protein processing in endoplasmic reticulum and lysosome pathway were significantly differentially expressed in the macrosomia placenta. Four lncRNAs were randomly chosen from the differentially expressed lncRNAs to validate the microarray data by quantitative polymerase chain reaction (qPCR). The qPCR results were consistent with the microarray data. In conclusion, lncRNAs were significantly differentially expressed in the placenta of macrosomia patients, and may contribute to the pathogenesis of macrosomia.

Keywords

lncRNAmacrosomiamicroarrayplacenta
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 9 , Issue 2 , April 2018 , pp. 191 - 197
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2017 

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References

1. Yang, S, Peng, A, Wei, S. Pre-pregnancy body mass index, gestational weight gain, and birth weight: a cohort study in China. PLoS One. 2015; 10, e0130101.Google Scholar
2. Koyanagi, A, Zhang, J, Dagvadorj, A. Macrosomia in 23 developing countries: an analysis of a multicountry, facility-based, cross-sectional survey. Lancet. 2013; 381, 476–483.CrossRefGoogle ScholarPubMed
3. Samaras, TT, Elrick, H, Storms, LH. Birthweight, rapid growth, cancer, and longevity: a review. J Natl Med Assoc. 2003; 95, 1170–1183.Google ScholarPubMed
4. Hermann, GM, Dallas, LM, Haskell, SE. Neonatal macrosomia is an independent risk factor for adult metabolic syndrome. Neonatology. 2010; 98, 238–244.Google Scholar
5. Barker, DJ. Intrauterine programming of adult disease. Mol Med Today. 1995; 1, 418–423.Google Scholar
6. Xie, M, Nie, FQ, Sun, M. Decreased long noncoding RNA SPRY4-IT1 contributing to gastric cancer cell metastasis partly via affecting epithelial-mesenchymal transition. J Transl Med. 2015; 13, 250.Google Scholar
7. Peng, W, Si, S, Zhang, Q. Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression. J Exp Clin Cancer Res. 2015; 34, 79.Google Scholar
8. Wei, G, Luo, H, Sun, Y. Transcriptome profiling of esophageal squamous cell carcinoma reveals a long noncoding RNA acting as a tumor suppressor. Oncotarget. 2015; 6, 17065–17080.Google Scholar
9. Luo, X, Pan, J, Wang, L. Epigenetic regulation of lncRNA connects ubiquitin-proteasome system with infection-inflammation in preterm births and preterm premature rupture of membranes. BMC Pregnancy Childbirth. 2015; 15, 35.Google Scholar
10. Satpathy, AT, Chang, HY. Long noncoding RNA in hematopoiesis and immunity. Immunity. 2015; 42, 792–804.Google Scholar
11. Akin, Y, Cömert, S, Turan, C. Macrosomic newborns: a 3-year review. Turk J Pediatr. 2010; 52, 378–383.Google ScholarPubMed
12. Moog, NK, Entringer, S, Heim, C. Influence of maternal thyroid hormones during gestation on fetal brain development. Neuroscience. 2017; 342, 68–100.CrossRefGoogle ScholarPubMed
13. Kridli, RT, Khalaj, K, Bidarimath, M. Placentation, maternal–fetal interface, and conceptus loss in swine. Theriogenology. 2016; 85, 135–447.Google Scholar
14. Redman, CW, Staff, AC. Preeclampsia, biomarkers, syncytiotrophoblast stress, and placental capacity. Am J Obstet Gynecol. 2015; 213(4 Suppl), S9.e1–S9.e4.Google Scholar
15. Sabri, A, Lai, D, D’Silva, A. Differential placental gene expression in term pregnancies affected by fetal growth restriction and macrosomia. Fetal Diagn Ther. 2014; 36, 173–180.CrossRefGoogle ScholarPubMed
16. Kim, EN, Yoon, BH, Lee, JY. Placental C4d deposition is a feature of defective placentation: observations in cases of preeclampsia and miscarriage. Virchows Arch. 2015; 466, 717–725.Google Scholar
17. Djebali, S, Davis, CA, Merkel, A. Landscape of transcription in human cells. Nature. 2012; 489, 101–108.CrossRefGoogle ScholarPubMed
18. Mercer, TR, Dinger, ME, Mattick, JS. Long non-coding RNAs: insights into functions. Nat Rev Genet. 2009; 10, 155–159.Google Scholar
19. Zou, Y, Jiang, Z, Yu, X. Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV_neo. PLoS One. 2013; 8, e79598.CrossRefGoogle ScholarPubMed
20. He, X, He, Y, Xi, B. LncRNAs expression in preeclampsia placenta reveals the potential role of lncRNAs contributing to preeclampsia pathogenesis. PLoS One. 2013; 8, e81437.CrossRefGoogle ScholarPubMed
21. Lee, J, Retamal, C, Cuitiño, L. Adaptor protein sorting nexin 17 regulates amyloid precursor protein trafficking and processing in the early endosomes. J Biol Chem. 2008; 283, 11501–11508.Google Scholar
22. Böttcher, RT, Stremmel, C, Meves, A. Sorting nexin 17 prevents lysosomal degradation of beta1 integrins by binding to the beta1-integrin tail. Nat Cell Biol. 2012; 14, 584–592.Google Scholar
23. Stockinger, W, Sailler, B, Strasser, V. The PX-domain protein SNX17 interacts with members of the LDL receptor family and modulates endocytosis of the LDL receptor. EMBO J. 2002; 21, 4259–4267.Google Scholar
24. Sotelo, P, Farfán, P, Benitez, ML. Sorting nexin 17 regulates ApoER2 recycling and reelin signaling. PLoS One. 2014; 9, e93672.Google Scholar
25. Baumann, MU, Schneider, H, Malek, A. Regulation of human trophoblast GLUT1 glucose transporter by insulin-like growth factor I (IGF-I). PLoS One. 2014; 9, e106037.Google Scholar
26. Baumann, MU, Zamudio, S, Illsley, NP. Hypoxic upregulation of glucose transporters in BeWo choriocarcinoma cells is mediated by hypoxia-inducible factor-1. Am J Physiol Cell Physiol. 2007; 293, C477–C485.Google Scholar
27. Gaither, K, Quraishi, AN, Illsley, NP. Diabetes alters the expression and activity of the human placental GLUT1 glucose transporter. J Clin Endocrinol Metab. 1999; 84, 695–701.Google Scholar
28. Caparrós, E, Munoz, P, Sierra-Filardi, E. DC-SIGN ligation on dendritic cells results in ERK and PI3K activation and modulates cytokine production. Blood. 2006; 107, 3950–3958.Google Scholar
29. Zhang, X, Chen, S, Xing, F. Regulation of invasion behavior of ERK-mediated trophoblast by focal adhesion kinase. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011; 36, 559–564.Google Scholar
30. Prakash, GJ, Suman, P, Morales Prieto, DM. Leukaemia inhibitory factor mediated proliferation of HTR-8/SVneo trophoblast cells is dependent on activation of extracellular signal-regulated kinase 1/2. Reprod Fertil Dev. 2011; 23, 714–724.CrossRefGoogle ScholarPubMed