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Metabolic programming in offspring of mice fed fructose during pregnancy and lactation

Published online by Cambridge University Press:  10 September 2021

Marina Lummertz Magenis ,
Adriani Paganini Damiani ,
Gustavo de Bem Silveira ,
Ligia Salvan Dagostin ,
Pamela Souza de Marcos ,
Emanuel de Souza ,
Laura de Roch Casagrande ,
Luiza Martins Longaretti ,
Paulo Cesar Lock Silveira  and
Vanessa Moraes de Andrade Open the ORCID record for Vanessa Moraes de Andrade [Opens in a new window]
Marina Lummertz Magenis
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Adriani Paganini Damiani
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Gustavo de Bem Silveira
Affiliation:
Laboratory of Experimental Pathophysiology, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Ligia Salvan Dagostin
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Pamela Souza de Marcos
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Emanuel de Souza
Affiliation:
Course of Biomedicine, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Laura de Roch Casagrande
Affiliation:
Laboratory of Experimental Pathophysiology, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Luiza Martins Longaretti
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Paulo Cesar Lock Silveira
Affiliation:
Laboratory of Experimental Pathophysiology, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
Vanessa Moraes de Andrade*
Affiliation:
Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, University of Southern Santa Catarina – UNESC, Criciúma, SC, Brazil
*
Address for correspondence: Vanessa Moraes de Andrade, Laboratory of Translational Biomedicine, Graduate Program of Health Sciences, Department of Health Sciences, University of Southern Santa Catarina, UNESC, 1105, Universitária Rd, 88806000, Criciúma, SC, Brazil. Email: [email protected]
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Abstract

Fructose (C6H12O6), also known as levulose, is a hexose. Chronic consumption of fructose may be associated with increased intrahepatic fat concentration and the development of insulin resistance as well as an increase in the prevalence of nonalcoholic fatty liver disease and hyperlipidemia during pregnancy. Despite the existence of many studies regarding the consumption of fructose in pregnancy, its effects on fetuses have not yet been fully elucidated. Therefore, the objective of this study was to evaluate the genetic and biochemical effects in offspring (male and female) of female mice treated with fructose during pregnancy and lactation. Pairs of 60-day-old Swiss mice were used and divided into three groups; negative control and fructose, 10%/l and 20%/l doses of fructose groups. After offspring birth, the animals were divided into six groups: P1 and P2 (males and females), water; P3 and P4 (males and females) fructose 10%/l; and P5 and P6 (males and females) fructose 20%/l. At 30 days of age, the animals were euthanized for genetic and biochemical assessments. Female and male offspring from both dosage groups demonstrated genotoxicity (evaluated through comet assay) and oxidative stress (evaluated through nitrite concentration, sulfhydril content and superoxide dismutase activity) in peripheral and brain tissues. In addition, they showed nutritional and metabolic changes due to the increase in food consumption, hyperglycemia, hyperlipidemia, and metabolic syndrome. Therefore, it is suggested that high consumption of fructose by pregnant female is harmful to their offspring. Thus, it is important to carry out further studies and make pregnant women aware of excessive fructose consumption during this period.

Keywords

Fructosepregnancyoffspringgenotoxicitybiochemistry
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 13 , Issue 4 , August 2022 , pp. 441 - 454
Copyright
© The Author(s), 2021. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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