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Maternal high-fat feeding in pregnancy programs atherosclerotic lesion size in the ApoE*3 Leiden mouse

Published online by Cambridge University Press:  02 February 2016

E. J. Tarling ,
K. J. P. Ryan ,
R. Austin ,
S. J. Kugler ,
A. M. Salter  and
S. C. Langley-Evans
E. J. Tarling
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
K. J. P. Ryan
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
R. Austin
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
S. J. Kugler
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
A. M. Salter
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
S. C. Langley-Evans*
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK
*
*Address for correspondence: Professor S. C. Langley-Evans, School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, UK. (Email [email protected])
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Abstract

Periods of rapid growth seen during the early stages of fetal development, including cell proliferation and differentiation, are greatly influenced by the maternal environment. We demonstrate here that over-nutrition, specifically exposure to a high-fat diet in utero, programed the extent of atherosclerosis in the offspring of ApoE*3 Leiden transgenic mice. Pregnant ApoE*3 Leiden mice were fed either a control chow diet (2.8% fat, n=12) or a high-fat, moderate-cholesterol diet (MHF, 19.4% fat, n=12). Dams were fed the chow diet during the suckling period. At 28 days postnatal age wild type and ApoE*3 Leiden offspring from chow or MHF-fed mothers were fed either a control chow diet (n=37) or a diet rich in cocoa butter (15%) and cholesterol (0.25%), for 14 weeks to induce atherosclerosis (n=36). Offspring from MHF-fed mothers had 1.9-fold larger atherosclerotic lesions (P<0.001). There was no direct effect of prenatal diet on plasma triglycerides or cholesterol; however, transgenic ApoE*3 Leiden offspring displayed raised cholesterol when on an atherogenic diet compared with wild-type controls (P=0.031). Lesion size was correlated with plasma lipid parameters after adjustment for genotype, maternal diet and postnatal diet (R2=0.563, P<0.001). ApoE*3 Leiden mothers fed a MHF diet developed hypercholesterolemia (plasma cholesterol two-fold higher than in chow-fed mothers, P=0.011). The data strongly suggest that maternal hypercholesterolemia programs later susceptibility to atherosclerosis. This is consistent with previous observations in humans and animal models.

Keywords

animaldevelopmental stagefetussmall animals
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 7 , Issue 3: Themed Issue: Ibero-American DOHaD Society , June 2016 , pp. 290 - 297
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2016 

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