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Green tea extract intake during lactation modified cardiac macrophage infiltration and AMP-activated protein kinase phosphorylation in weanling rats from undernourished mother during gestation and lactation

Published online by Cambridge University Press:  06 December 2016

E. Matsumoto ,
S. Kataoka ,
Y. Mukai ,
M. Sato  and
S. Sato
E. Matsumoto
Affiliation:
Graduate School of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan
S. Kataoka
Affiliation:
Graduate School of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan
Y. Mukai
Affiliation:
School of Nutrition and Dietetics, Faculty of Health and Social Work, Kanagawa University of Human Services, Kanagawa, Japan
M. Sato
Affiliation:
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan
S. Sato*
Affiliation:
Graduate School of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan
*
*Address for correspondence: Dr. S. Sato, Graduate School of Health Sciences, Aomori University of Health and Welfare, Mase 58-1, Hamadate, Aomori 030-8505, Japan. (Email [email protected])
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Abstract

Maternal dietary restriction is often associated with cardiovascular disease in offspring. The aim of this study was to investigate the effect of green tea extract (GTE) intake during lactation on macrophage infiltration, and activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and serine-threonine kinase Akt (Akt) in the hearts of weanlings exposed to maternal dietary protein restriction. Pregnant Wistar rats were fed control (C) or low-protein diets (LP) throughout gestation. Following delivery, the dams received a control or a GTE-containing control diet during lactation: control diet during gestation and lactation (CC), low-protein diet during gestation and lactation (LPC), low-protein diet during gestation and 0.12% GTE-containing low-protein diet during lactation (LPL), and low-protein diet during gestation and 0.24% GTE-containing low-protein diet during lactation (LPH). The female offspring were sacrificed at day 22. Biochemical parameters in the plasma, macrophage infiltration, degree of fibrosis and expression levels of AMPK and Akt were examined. The plasma insulin level increased in LPH compared with LPC. Percentage of the fibrotic areas and the number of macrophages in LPC were higher than those in CC. Conversely, the fibrotic areas and the macrophage number in LPH were smaller (21 and 56%, respectively) than those in LPC. The levels of phosphorylated AMPK in LPL and LPH, and Akt in LPH were greater than those in LPC. In conclusion, maternal protein restriction may induce macrophage infiltration and the decrease of insulin levels. However, GTE intake during lactation may suppress macrophage infiltration and restore insulin secretion function via upregulation of AMPK and insulin signaling in weanlings.

Keywords

Green tea extractmacrophageheartoffspringmaternal protein restriction
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 8 , Issue 2 , April 2017 , pp. 178 - 187
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2016 

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