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Effect of maternal and postnatal cocoa supplementation on testicles of adult Wistar rats

Published online by Cambridge University Press:  20 July 2020

Juliana Lima ,
Letícia Cardoso ,
Gabrielle Rocha ,
Caroline Fernandes-Santos ,
Diogo de Souza ,
Francisco Jose Sampaio  and
Bianca Gregorio Open the ORCID record for Bianca Gregorio [Opens in a new window]
Juliana Lima
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Letícia Cardoso
Affiliation:
Faculty of Nutrition Emília de Jesus Ferreiro, Department of Nutrition and Dietetics, UFF, Niterói, Brazil
Gabrielle Rocha
Affiliation:
Faculty of Nutrition Emília de Jesus Ferreiro, Department of Nutrition and Dietetics, UFF, Niterói, Brazil
Caroline Fernandes-Santos
Affiliation:
Laboratory Multiuser of Biomedical Research, Department of Basic Sciences, UFF, Nova Friburgo, Brazil
Diogo de Souza
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Francisco Jose Sampaio
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
Bianca Gregorio*
Affiliation:
Urogenital Research Unit, Department of Anatomy, UERJ, Rio de Janeiro, Brazil
*
Address for correspondence: Bianca Gregorio, Urogenital Research Unit, Department of Anatomy, Biomedical Center, State University of Rio de Janeiro, Av. 28 de setembro 87 (fds), 20551-030Rio de Janeiro, RJ, Brazil. Email: [email protected]
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Abstract

Early weaning can lead to changes in the morphology of organs in adulthood, and the consumption of functional foods during lactation and postnatal life is believed to prevent these changes. However, it is not known if early weaning affects testicular morphology and if the use of cocoa can prevent that. We studied the effects of maternal and postnatal supplementation of cocoa powder on the testicular morphology of early weaned adult rats. The animals were divided into four groups (n = 6 each), control group, cocoa control group, early weaning (EW) group, and cocoa early weaning (EWCa) group, and were analyzed for 90 d, after which they were euthanized. The animals from the EW group showed a reduction in the tubular diameter and height of the seminiferous epithelium, a decrease in epithelial surface density (Sv), and an increase in the lumen and proper tunic. However, the animals from the EWCa group showed an increase in the diameter and height of the epithelium, an increase in the epithelium Sv, and a decrease in the lumen and the proper tunic. The early weaning promotes morphological changes in the testicles; however, supplementation with cocoa powder can preserve the testicular histoarchitecture.

Keywords

early weaningtesticlefetal programmingcocoa
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 3 , June 2021 , pp. 436 - 442
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Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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References

Amaral, LJX, dos Santos Sales, S, Pinto, DPdSR, et al. Fatores que influenciam na interrupção do aleitamento materno exclusivo em nutrizes. Rev Gaúcha Enferm. 2015; 36, 127–134.CrossRefGoogle Scholar
Prado, CVC, Fabbro, MRC, Ferreira, GI. Desmame precoce na perspectiva de puérperas: uma abordagem dialógica. Texto & Contexto-Enfermagem. 2016; 25, e1580015.Google Scholar
Xavier, J, Scomparin, D, Ribeiro, P, et al. Metabolic imprinting: causes and consequences. Visão Acadêmica. 2016; 16, 33–41.CrossRefGoogle Scholar
Souza, FSd, Taralli, G. Programação metabólica e prevenção da obesidade. Pediatr mod. 2012; 48, lil-66318.Google Scholar
Hahn, M, Baierle, M, Charão, MF, et al. Polyphenol-rich food general and on pregnancy effects: a review. Drug Chem Toxicol. 2017; 40, 368–374.CrossRefGoogle ScholarPubMed
Decroix, L, van Schuerbeek, P, Tonoli, C, et al. The effect of acute cocoa flavanol intake on the BOLD response and cognitive function in type 1 diabetes: a randomized, placebo-controlled, double-blinded cross-over pilot study. Psychopharmacology (Berl). 2019; 236, 3421–3428.CrossRefGoogle ScholarPubMed
Rassaf, T, Rammos, C, Hendgen-Cotta, UB, et al. Vasculo protective effects of dietary cocoa flavanols in patients on hemodialysis: a double-blind, randomized, placebo-controlled trial. Clin J Am Soc Nephrol. 2016; 11, 108–118.CrossRefGoogle Scholar
Gu, Y, Hurst, WJ, Stuart, DA, et al. Inhibition of key digestive enzymes by cocoa extracts and procyanidins. J Agric Food Chem. 2011; 59, 5305–5311.CrossRefGoogle ScholarPubMed
Campos-Silva, P, Costa, WS, Sampaio, FJ, et al. Prenatal and/or postnatal high-fat diet alters testicular parameters in adult Wistar Albino rats. Histol Histopathol. 2018; 33, 407–416.Google ScholarPubMed
Albus, U. Guide for the Careand Use of Laboratory Animals (8th ed), 2012. SAGE Publications Sage UK, London, England.Google Scholar
Langley-Evans, S, Phillips, G, Benediktsson, R, et al. Protein intake in pregnancy, placental glucocorticoid metabolism and the programming of hypertension in the rat. Placenta. 1996; 17, 169–172.CrossRefGoogle ScholarPubMed
Álvarez-Cilleros, D, Ramos, S, López-Oliva, ME, et al. Cocoa diet modulates gut microbiota composition and improves intestinal health in Zucker diabetic rats. Food Res Int. 2020; 132, 109058.CrossRefGoogle ScholarPubMed
Álvarez-Cilleros, D, López-Oliva, ME, Martín, MÁ, et al. Cocoa ameliorates renal injury in Zucker diabetic fatty rats by preventing oxidative stress, apoptosis and inactivation of autophagy. Food Funct. 2019; 10, 7926–7939.CrossRefGoogle ScholarPubMed
Friedewald, WT, Levy, RI, Fredrickson, DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18, 499–502.CrossRefGoogle ScholarPubMed
Ribeiro, CT, De Souza, DB, Medeiros, JL Jr, et al. Pneumoperitoneum induces morphological alterations in the rat testicle. Acta Cir Bras. 2013; 28, 419–422.CrossRefGoogle ScholarPubMed
De Souza, DB, Silva, D, Cortez, CM, et al. Effects of chronic stress on penile corpus cavernosum of rats. J Androl. 2012; 33, 735–739.CrossRefGoogle ScholarPubMed
Shekarforoush, S, Ebrahimi, Z, Hoseini, M. Sodium metabisulfite-induced changes on testes, spermatogenesis and epididymal morphometric values in adult rats. Int J Reprod Biomed (Yazd). 2015; 13, 765–770.Google ScholarPubMed
Pinto-Fochi, ME, Pytlowanciv, EZ, Reame, V, et al. A high-fat diet fed during different periods of life impairs steroidogenesis of rat Leydig cells. Reproduction. 2016; 6, 795–808.CrossRefGoogle Scholar
Kotsampasi, BC, Balaskas, G, Papadomichelakis, G, Chadio, SE. Reduced Sertoli cell number and altered pituitary responsiveness in male lambs undernourished in utero. Anim Reprod Sci. 2009; 114, 135–147.CrossRefGoogle ScholarPubMed
Greenberg, JA, Buijsse, B. Habitual chocolate consumption may increase body weight in a dose-response manner. PLoS One. 2013; 8, e70271.CrossRefGoogle Scholar
Rodríguez-Lagunas, MJ, Vicente, F, Pereira, P, et al. Relationship between cocoa intake and healthy status: a pilot study in university students. Molecules. 2019; 24, 812.CrossRefGoogle ScholarPubMed
Kim, AR, Kim, KM, Byun, MR, et al. Catechins activate muscle stem cells by Myf5 induction and stimulate muscle regeneration. Biochem Biophys Res Commun. 2017; 489, 142–148.CrossRefGoogle ScholarPubMed
Yoshioka, Y, Yamashita, Y, Kishida, H, et al. Licorice flavonoid oil enhance smuscle mass in KK-Ay mice. Life Sci. 2018; 205, 91–96.CrossRefGoogle Scholar
Brugman, S, Visser, J, Hillebrands, JL, et al. Prolonged exclusive breastfeeding reduces autoimmune diabetes incidence and increases regulatory T-cell frequency in bio-breeding diabetes-prone rats. Diabetes Metab Res Rev. 2009; 25, 380–387.CrossRefGoogle ScholarPubMed
Sánchez, D, Moulay, L, Muguerza, B, et al. Effect of a soluble cocoa fiber-enriched diet in Zucker fatty rats. J Med Food. 2010; 13, 621–628.CrossRefGoogle ScholarPubMed
Álvarez-Cilleros, D, López-Oliva, E, Goya, L, et al. Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis. Food Chem Toxicol. 2019; 127, 101–109.CrossRefGoogle ScholarPubMed
Mustiga, GM, Morrissey, J, Stack, JC, et al. Identification of climate and genetic factors that control fat content and fatty acid composition of Theobroma cacao L. beans. Front Plant Sci. 2019; 10, 1159.CrossRefGoogle ScholarPubMed
Faria e Souza, BS, Carvalho, HO, Taglialegna, T, et al. Effect of Euterpe oleracea Mart.(Acai) Oil on dyslipidemia caused by cocos nucifera L. Saturated Fat in WistarRats. J Med Food. 2017; 20, 830–837.CrossRefGoogle Scholar
Ooi, EM, Watts, GF, Ng, TW, et al. Effect of dietary fatty acids on human lipoprotein metabolism: a comprehensive update. Nutrients. 2015; 7, 4416–4425.CrossRefGoogle ScholarPubMed
de Albuquerque Maia, L, Lisboa, PC, de Oliveira, E, et al. Bone metabolism in obese rats programmed by early weaning. Metabolism. 2014; 63, 352–364.CrossRefGoogle ScholarPubMed
Clough, BH, Ylostalo, J, Browder, E, et al. Theobromine upregulates osteogenesis by human mesenchymal stemcells in vitro and accelerates bone development in rats. Calcif Tissue Int. 2017; 100, 298–310.CrossRefGoogle Scholar
Seem, SA, Yuan, YV, Tou, JC. Chocolate and chocolate constituents influence bone health and osteoporosis risk. Nutrition. 2019; 65, 74–84.CrossRefGoogle ScholarPubMed
Kanter, M, Aktas, C, Erboga, M. Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis. Food Chem Toxicol. 2012; 50, 719–725.CrossRefGoogle ScholarPubMed
Rashid, K, Sil, PC. Curcumin ameliorates testicular damage in diabetic rats by suppressing cellular stress-mediated mitochondria and endoplasmic reticulum-dependent apoptotic death. Biochim Biophys Acta. 2015; 1852, 70–82.CrossRefGoogle ScholarPubMed
Azari, O, Gholipour, H, Kheirandish, R, et al. Study of the protective effect of vitamin C on testicular tissue following experimental unilateral cryptorchidism in rats. Andrologia. 2014; 46, 495–503.CrossRefGoogle ScholarPubMed