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Characterization of the role of microRNA-517a expression in low birth weight infants

Published online by Cambridge University Press:  26 June 2013

G. Y. Song ,
W. W. Song ,
Y. Han ,
D. Wang  and
Q. Na
G. Y. Song
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
W. W. Song*
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
Y. Han
Affiliation:
Department of Gynecology and Obstetrics, Shenyang Women and Children's Hospital, Shenyang, Liaoning Province, China
D. Wang
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
Q. Na
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
*
*Address for correspondence: W. W. Song, Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Shenyang, Liaoning 110004, P.R. China. (Email [email protected])
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Abstract

The purpose of this study was to analyze the expression of the placenta-specific microRNA miR-517a in maternal serum and in placental tissue from low birth weight newborns and try to detect the effects of miR-517a expression on invasion potential of trophoblasts. Placental tissue and maternal serum were collected from both low birth weight newborns (n = 10) and normal birth weight newborns (n = 20). Expression of miR-517a was assessed in placenta and serum samples by real-time qRT-PCR. In addition, human trophoblast HTR8/SVneo cells were transfected with a miR-517a 2′-O-methyl oligonucleotide or a negative control RNA, and invasion was measured using transwell migration assays. Expression of miR-517a was significantly increased in placentas from low birth weight newborns (61.79 ± 23.06) in comparison with those of normal birth weight newborns (5.01 ± 1.97; P < 0.05). The expression of miR-517a was also increased in maternal serum isolated from the low birth weight newborn (25.78 ± 8.69) compared with the normal birth weight newborn (3.21 ± 1.07; P < 0.05). Overexpression of miR-517a significantly inhibited invasion of HTR8/SVneo cells (P < 0.05). These data indicate that miR-517a overexpression could potentially lead to low birth weight, likely through the inhibition of trophoblast invasion.

Keywords

birth weightmiR-517aplacentatrophoblast invasion
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 4 , Issue 6 , December 2013 , pp. 522 - 526
Copyright
Copyright © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2013 

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References

1.Calkins, K, Devaskar, SU. Fetal origins of adult disease. Curr Probl Pediatr Adolesc Health Care. 2011; 41, 158–176.CrossRefGoogle ScholarPubMed
2.Lee, RC, Ambros, V. An extensive class of small RNAs in Caenorhabditis elegans. Science. 2001; 294, 862–864.CrossRefGoogle ScholarPubMed
3.Luo, SS, Ishibashi, O, Ishikawa, G, et al. Human villous trophoblasts express and secrete placenta-specific microRNAs into maternal circulation via exosomes. Biol Reprod. 2009; 81, 717–729.CrossRefGoogle ScholarPubMed
4.Grimwood, J, Gordon, LA, Olsen, A, et al. The DNA sequence and biology of human chromosome 19. Nature. 2004; 428, 529–535.CrossRefGoogle ScholarPubMed
5.Fang, JH, Zhou, HC, Zeng, CX, et al. MicroRNA-29b suppresses tumor angiogenesis, invasion, and metastasis by regulating matrix metalloproteinase 2 expression. Hepatology. 2011; 54, 1729–1740.CrossRefGoogle ScholarPubMed
6.Wu, DG, Wang, YY, Fan, LG, et al. MicroRNA-7 regulates glioblastoma cell invasion via targeting focal adhesion kinase expression. Chin Med J (Engl). 2011; 124, 2616–2621.Google ScholarPubMed
7.Fang, L, Yang, N, Ma, J, et al. MicroRNA-1301-mediated inhibition of tumorigenesis. Oncol Rep. 2012; 27, 929–934.CrossRefGoogle ScholarPubMed
8.Yu, Z, Han, S, Hu, P, et al. Potential role of maternal serum microRNAs as a biomarker for fetal congenital heart defects. Med Hypotheses. 2011; 76, 424–426.CrossRefGoogle ScholarPubMed
9.Ong, KK, Dunger, DB. Perinatal growth failure: the road to obesity, insulin sistance and cardiovascular disease in adults. Best Pract Res Clin Endocrinol Metab. 2002; 16, 191–207.CrossRefGoogle Scholar
10.Varvarigou, AA. Intrauterine growth restriction as a potential risk factor for disease onset in adulthood[J]. J Pediatr Endocrinol Metab. 2010; 23, 215–224.CrossRefGoogle ScholarPubMed
11.Calkins, K, Devaskar, SU. Fetal origins of adult disease. Curr Probl Pediatr Adolesc Health Care. 2011; 41, 158–176.CrossRefGoogle ScholarPubMed
12.Yang, W, Weiwei, S, Jingli, F, et al. The effects of leptin,insulin and IGF-2 on the expressions of PKB and P70S6 K protein in the trophoblastic cells and the possible mechanism. Prog Obstet Gynecol. 2010, 16–19.Google Scholar
13.Xiao-Xiao, L, Wei-wei, S. Relationship between the expression of H19 and IGF-II imprinted gene in newborn placenta and the birth body mass. J China Med Univ. 2009, 42–44.Google Scholar
14.Amiruddin, R, Yusuf, I. Influence of antenatal care, placental weight and genetic variation on low birth weight, Makassar Indonesia. Asia Pac J Public Health. 2008; 20(Suppl), 15–17.Google ScholarPubMed
15.Roberts, CT. IFPA Award in Placentology Lecture: Complicated interactions between genes and the environment in placentation, pregnancy outcome and long term health. Placenta. 2010; 31(Suppl), S47–S53.CrossRefGoogle ScholarPubMed
16.Herman, JG, Meadows, GG. Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells. Int J Oncol. 2007; 30, 1231–1238.Google ScholarPubMed
17.Graham, CH, Hawley, TS, Hawley, RG, et al. Establishment and characterization of human trophoblast cells with extended life span. Exp Cell Res. 1993; 206, 204–211.CrossRefGoogle Scholar