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The impact of maternal protein restriction during perinatal life on the response to a septic insult in adult rats

Published online by Cambridge University Press:  23 December 2020

Reza Khazaee ,
Anastasiya Vinokurtseva ,
Lynda A. McCaig ,
Cory Yamashita ,
Daniel B. Hardy ,
Edith Arany  and
Ruud A. W. Veldhuizen Open the ORCID record for Ruud A. W. Veldhuizen [Opens in a new window]
Reza Khazaee
Affiliation:
Biotron Research Centre, London, Ontario, Canada
Anastasiya Vinokurtseva
Affiliation:
Department of Pathology and Laboratory Medicine, The University of Western Ontario, London, Ontario, Canada
Lynda A. McCaig
Affiliation:
Lawson Health Research Institute, London, Ontario, Canada
Cory Yamashita
Affiliation:
Department of Physiology & Pharmacology, The University of Western Ontario, London, Ontario, Canada Department of Medicine, The University of Western Ontario, London, Ontario, Canada Lawson Health Research Institute, London, Ontario, Canada
Daniel B. Hardy
Affiliation:
Department of Physiology & Pharmacology, The University of Western Ontario, London, Ontario, Canada Department of Obstetrics & Gynecology, The University of Western Ontario, London, Ontario, Canada Lawson Health Research Institute, London, Ontario, Canada
Edith Arany
Affiliation:
Department of Pathology and Laboratory Medicine, The University of Western Ontario, London, Ontario, Canada Lawson Health Research Institute, London, Ontario, Canada
Ruud A. W. Veldhuizen*
Affiliation:
Department of Physiology & Pharmacology, The University of Western Ontario, London, Ontario, Canada Department of Obstetrics & Gynecology, The University of Western Ontario, London, Ontario, Canada Lawson Health Research Institute, London, Ontario, Canada
*
*Address for correspondence: Ruud Veldhuizen, Lawson Health Research Institute, E4-110268 Grosvenor St., London, ON, N6A 4V2, Canada. Email: [email protected]
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Abstract

Although abundant evidence exists that adverse events during pregnancy lead to chronic conditions, there is limited information on the impact of acute insults such as sepsis. This study tested the hypothesis that impaired fetal development leads to altered organ responses to a septic insult in both male and female adult offspring. Fetal growth restricted (FGR) rats were generated using a maternal protein-restricted diet. Male and female FGR and control diet rats were housed until 150–160 d of age when they were exposed either a saline (control) or a fecal slurry intraperitoneal (Sepsis) injection. After 6 h, livers and lungs were analyzed for inflammation and, additionally, the amounts and function of pulmonary surfactant were measured. The results showed increases in the steady-state mRNA levels of inflammatory cytokines in the liver in response to the septic insult in both males and females; these responses were not different between FGR and control diet groups. In the lungs, cytokines were not detectable in any of the experimental groups. A significant decrease in the relative amount of surfactant was observed in male FGR offspring, but this was not observed in control males or in female animals. Overall, it is concluded that FGR induced by maternal protein restriction does not impact liver and lung inflammatory response to sepsis in either male or female adult rats. An altered septic response in male FGR offspring with respect to surfactant may imply a contribution to lung dysfunction.

Keywords

Sepsisfetal growth restrictioninflammationlungliver
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 6 , December 2021 , pp. 915 - 922
Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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Footnotes

*

Authors made equal contributions to this manuscript.

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