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Effects of prenatal bisphenol-A exposure and postnatal overfeeding on cardiovascular function in female sheep

Published online by Cambridge University Press:  04 November 2016

S. M. J. MohanKumar ,
T. D. Rajendran ,
A. K. Vyas ,
V. Hoang ,
N. Asirvatham-Jeyaraj ,
A. Veiga-Lopez ,
N. B. Olivier ,
V. Padmanabhan  and
P. S. MohanKumar
S. M. J. MohanKumar
Affiliation:
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA
T. D. Rajendran
Affiliation:
Department of Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA
A. K. Vyas
Affiliation:
Pediatrics and Human Development, Michigan State University, East Lansing, MI, USA
V. Hoang
Affiliation:
Pediatrics and Human Development, Michigan State University, East Lansing, MI, USA
N. Asirvatham-Jeyaraj
Affiliation:
Pharmacology & Toxicology, Michigan State University, East Lansing, MI, USA
A. Veiga-Lopez
Affiliation:
Department of Animal Sciences, Michigan State University, East Lansing, MI, USA Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA
N. B. Olivier
Affiliation:
Department of Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA
V. Padmanabhan
Affiliation:
Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA
P. S. MohanKumar*
Affiliation:
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA, USA
*
*Address for correspondence: Professor P. S. MohanKumar, Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Rm H370, Athens, GA 30602, USA. (Email [email protected])
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Abstract

Bisphenol-A (BPA) is a widely used endocrine-disrupting chemical. Prenatal exposure to BPA is known to affect birth weight, but its impact on the cardiovascular system has not been studied in detail. In this study, we investigated the effects of prenatal BPA treatment and its interaction with postnatal overfeeding on the cardiovascular system. Pregnant sheep were given daily subcutaneous injections of corn oil (control) or BPA (0.5 mg/kg/day in corn oil) from day 30 to day 90 of gestation. A subset of female offspring of these dams were overfed to increase body weight to ~30% over that of normal fed controls. Cardiovascular function was assessed using non-invasive echocardiography and cuff blood pressure (BP) monitoring at 21 months of age. Ventricular tissue was analyzed for gene expression of cardiac markers of hypertrophy and collagen at the end of the observation period. Prenatal BPA exposure had no significant effect on BP or morphometric measures. However, it increased atrial natriuretic peptide gene expression in the ventricles and reduced collagen expression in the right ventricle. Overfeeding produced a marked increase in body weight and BP. There were compensatory increases in left ventricular area and internal diameter. Prenatal BPA treatment produced a significant increase in interventricular septal thickness when animals were overfed. However, it appeared to block the increase in BP and left ventricular area caused by overfeeding. Taken together, these results suggest that prenatal BPA produces intrinsic changes in the heart that are capable of modulating morphological and functional parameters when animals become obese in later life.

Keywords

blood pressurecardiac gene expressioncardio-metabolic programmingechocardiographyendocrine disruptors
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 8 , Issue 1 , February 2017 , pp. 65 - 74
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2016 

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