Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-26T08:47:55.906Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of monoclonal antibodies for radioimmunoassay of water buffalo milk progesterone

Published online by Cambridge University Press:  01 June 2009

Rosanna Capparelli ,
Domenico Iannelli  and
Aldo Bordi
Rosanna Capparelli
Affiliation:
Institute of Animal Production, University of Naples, 100-Portici 80055, Naples, Italy
Domenico Iannelli
Affiliation:
Institute of Animal Production, University of Naples, 100-Portici 80055, Naples, Italy
Aldo Bordi
Affiliation:
Institute of Animal Production, University of Naples, 100-Portici 80055, Naples, Italy
Get access Rights & Permissions [Opens in a new window]

Summary

In order to standardize a radioimmunoassay of milk progesterone as a routine method for confirmation of oestrus and diagnosis of pregnancy in water buffalo, monoclonal antibodies against progesterone were produced. Hybridomas were prepared by fusing spleen cells from a Balb/c mouse immunized with progesterone 11α-hemisuccinate–bovine serum albumin conjugate with the mouse myeloma cell line NS-1. Thirty wells out of 94 secreted anti-progesterone antibodies. Of the ten independent hybridomas derived, one (AF65) was suitable for the quantification of milk progesterone by radioimmunoassay. The tracer used in the assay was progesterone-11α-hemisuccinate ([2-125I]iodohistamine). The sensitivity of the assay was 50 pg/tube. The mean progesterone concentration at oestrus was 0·8±0·2 ng/ml increasing to 8·5±0·8 ng/ml 24 d later in pregnant animals.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 4 , November 1987 , pp. 471 - 477
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abraham, G. E. 1969 Solid-phase radioimmunoassay of estradiol-17β. Journal of Clinical Endocrinology and Metabolism 29 866–870CrossRefGoogle Scholar
Claus, R. & Rattenberger, E. 1979 Improved method for progesterone determination in milk fat. British Veterinary Journal 135 464–469CrossRefGoogle ScholarPubMed
Cockrill, W. R. 1974 The husbandry and health of the domestic buffalo. Rome: Food and Agriculture Organization of the United NationsGoogle Scholar
Eshhar, Z., Kim, J. B., Barnard, G., Collins, W. P., Gilad, S., Lindner, H. R. & Kohen, F. 1981 Use of monoclonal antibodies to pregnanediol–3α-glucuronide for the development of a solid phase chemi-luminescence immunoassay. Steroids 38 89–109CrossRefGoogle Scholar
Fantl, V. E. & Wang, D. Y. 1984 Simultaneous production of monoclonal antibodies to dehydro-epiandrosterone, oestradiol, progesterone and testosterone. Journal of Endocrinology 100 367–376CrossRefGoogle Scholar
Goding, J. W. 1976 Conjugation of antibodies with fluorochromes: modifications of the standard methods. Journal of Immunological Methods 13 215–226CrossRefGoogle ScholarPubMed
Gowan, E. W., Etches, R. J., Bryden, C. & King, J. 1982 Factors affecting accuracy of pregnancy diagnosis in cattle. Journal of Dairy Science 65 1294–1302CrossRefGoogle ScholarPubMed
Günzler, O., Rattenberger, E., Görlach, A., Hahn, R., Hocze, P., Claus, R. & Karg, H. 1979 Milk progesterone determination as applied to the confirmation of oestrus, the detection of cycling and as an aid to veterinarian and biotechnical measures in cows. British Veterinary Journal 135 541–549CrossRefGoogle Scholar
Hafez, E. S. E. 1953 Conception-rate and periodicity in the buffalo. Empire Journal of Experimental Agriculture 21 15–21Google Scholar
Heap, R. B., Holdswokth, R. J., Gadsby, J. E., Laing, J. A. & Walters, D. E. 1976 Pregnancy diagnosis in the cow from milk progesterone concentration. British Veterinary Journal 132 445–464CrossRefGoogle ScholarPubMed
Hengartner, H., Luzzati, A. L. & Schreier, M. 1978 Fusion of in vitro immunized lymphoid cells with X63Ag8. In Current Topics in Microbiology and Immunology vol. 81 pp. 92–99 (Eds Melchers, F., Potter, M. and Warner, N.). Berlin: Springer-VerlagGoogle Scholar
Hoffmann, B., Günzler, O., Hamburger, R. & Schmidt, W. 1976 Milk progesterone as a parameter for fertility control in cattle; methodological approaches and present status of application in Germany. British Veterinary Journal 132 469–476CrossRefGoogle ScholarPubMed
Hunter, W. M. & Greenwood, F. C. 1962 Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194 495–496CrossRefGoogle ScholarPubMed
Iannelli, D., Capparelli, R., Marziano, F., Scala, F. & Noviello, C. 1983 Production of hybridomas secreting monoclonal antibodies to the genus Fusarium. Mycotaxon 17 523–532Google Scholar
Kamonpatana, M., Koops, W., Van De Wiel, D. F. M., Ngramsuriyaroj, C. & Srisakwattana, K. 1982 Milk progesterone during oestrous cycle in buffalo. Proceedings of the 2nd International Water Buffalo Congress, Caserta pp. 354–359Google Scholar
Köhler, G. & Milstein, C. 1975 Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256 495–497CrossRefGoogle ScholarPubMed
Köhler, G., Howe, S. C. & Milstein, C. 1976 Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. European Journal of Immunology 6 292–295CrossRefGoogle ScholarPubMed
Parsons, G. H. 1981 Antibody-coated plastic tubes in radioimmunoassay. In Methods in Enzymology 73 224–239CrossRefGoogle Scholar
Pope, G. S., Majzlik, I., Ball, P. J. H. & Leaver, J. D. 1976 Use of progesterone concentrations in plasma and milk in the diagnosis of pregnancy in domestic cattle. British Veterinary Journal 132 497–506CrossRefGoogle ScholarPubMed
Salmon, S. E., Mackey, G. & Fudenberg, H. 1969 ‘Sandwich’ solid phase radioimmunoassay for the quantitative determination of human immunoglobulins. Journal of Immunology 103 129–137CrossRefGoogle ScholarPubMed
Staehelin, T., Durrer, B., Schmidt, J., Takacs, B., Stocker, J., Miggiano, V., Stähli, C., Rubinstein, M., Levy, W. P., Hershberg, R. & Pestka, S. 1981 Production of hybridomas secreting monoclonal antibodies to the human leukocyte interferons. Proceedings of National Academy of Sciences of the USA 78 1848–1852CrossRefGoogle Scholar
Stähli, C., Staehelin, T., Miggiano, V., Schmidt, J. & Haring, P. 1980 High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis. Journal of Immunological Methods 32 297–304CrossRefGoogle ScholarPubMed
Takacs, B. 1979 Electrophoresis of proteins in polyacrylamide slab gels. In Immunological Methods pp. 81–105 (Eds Lefkovits, I. and Pernis, B.). New York: Academic PressCrossRefGoogle Scholar
White, A., Anderson, D. E. & Daly, J. R. 1982 Production of a highly specific monoclonal antibody to progesterone. Journal of Clinical Endocrinology and Metabolism 54 205–207CrossRefGoogle ScholarPubMed
Wright, L. J., Feinstein, A., Heap, R. B., Saunders, J. C., Bennett, R. C. & Wang, M. Y. 1982 Progesterone monoclonal antibody pregnancy in mice. Nature 295 415–417CrossRefGoogle ScholarPubMed