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Updating on the fungal composition in Sardinian sheep's milk by culture-independent methods

Published online by Cambridge University Press:  26 March 2014

Simona Panelli ,
Eva Brambati ,
Cesare Bonacina  and
Maria Feligini
Simona Panelli
Affiliation:
Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano ‘Lazzaro Spallanzani’, Località La Quercia, 26027 Rivolta d'Adda (Cremona), Italy
Eva Brambati
Affiliation:
Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano ‘Lazzaro Spallanzani’, Località La Quercia, 26027 Rivolta d'Adda (Cremona), Italy
Cesare Bonacina
Affiliation:
Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano ‘Lazzaro Spallanzani’, Località La Quercia, 26027 Rivolta d'Adda (Cremona), Italy
Maria Feligini*
Affiliation:
Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano ‘Lazzaro Spallanzani’, Località La Quercia, 26027 Rivolta d'Adda (Cremona), Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

This work applies culture-independent methods for the characterization of fungal populations (yeasts and moulds) naturally occurring in Sardinian ewe's milk sampled in the Italian areas with the largest dairy production (Sardinia and Lazio regions). Sequences of the D1/D2 variable domains at the 5′ end of the 26S rRNA gene were obtained by amplification of DNA directly isolated from milk, and this allowed identification of a total of 6 genera and 15 species of fungi. Among the 6 identified genera Geotrichum spp., Candida spp., Phaeosphaeriopsis spp., Pestalotiopsis spp. and Cladosporium spp. belong to the phylum of Ascomycota, while Cryptococcus spp. is part of the phylum of Basidiomycota. In particular, two genera (Pestalotiopsis and Phaeosphaeriopsis) and two species (Plectosphaerella cucumerina and Pryceomyces carsonii) have never been reported in dairy ecosystems before. Results provide evidence that several moulds and yeasts, previously described only in ovine cheeses, are transferred directly from raw milk. The knowledge of fungal consortia inhabiting sheep raw milk is a particularly relevant issue because several species are directly involved in cheese making and ripening, determining the typical aroma. On the other hand, spoilage yeasts and moulds are involved in anomalous fermentation of cheese and may be responsible for considerable economic losses and serious risks for consumers' health.

Keywords

Fungiyeastsmouldsmilk26S rRNA
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 2 , May 2014 , pp. 233 - 237
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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References

Andrade, MJ, Rodríguez, M, Sánchez, B, Aranda, E & Córdoba, JJ 2006 DNA typing methods for differentiation of yeasts related to dry-cured meat products. International Journal of Food Microbiology 107 48–58Google Scholar
Atkins, SD, Clark, IM, Sosnowska, D, Hirsch, PR & Kerry, BR 2003 Detection and quantification of Plectosphaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media, and baiting. Applied and Environmental Microbiology 69 4788–4793Google Scholar
Boutrou, R & Guéguen, M 2005 Interests in Geotrichum candidum for cheese technology. International Journal of Food Microbiology 102 1–20CrossRefGoogle ScholarPubMed
Callon, C, Delbès, C, Duthoit, F & Montel, MC 2006 Application of SSCP-PCR fingerprinting to profile the yeast community in raw milk Salers cheeses. Systematic and Applied Microbiology 29 172–180Google Scholar
Callon, C, Duthoit, F, Delbès, C, Ferrand, M, Le Frileux, Y, De Crémoux, R & Montel, MC 2007 Stability of microbial communities in goat milk during a lactation year: molecular approaches. Systematic and Applied Microbiology 30 547–560Google Scholar
Capece, A & Romano, P 2009 Pecorino di Filiano cheese as a selective habitat for the yeast species, Debaryomyces hansenii. International Journal of Food Microbiology 132 180–184CrossRefGoogle ScholarPubMed
Coda, R, Rizzello, CG, Di Cagno, R, Trani, A, Cardinali, G & Gobbetti, M 2013 Antifungal activity of Meyerozyma guilliermondii: identification of active compounds synthesized during dough fermentation and their effect on long-term storage of wheat bread. Food Microbiology 33 243–251Google Scholar
Cosentino, S, Fadda, ME, Deplano, M, Mulargia, AF & Palmas, F 2001 Yeasts associated with Sardinian ewe's dairy products. International Journal of Food Microbiology 69 53–58Google Scholar
Crawshaw, WM, MacDonald, NR & Duncan, G 2005 Outbreak of Candida rugosa mastitis in a dairy herd after intramammary antibiotic treatment. Veterinary Records 156 812–813CrossRefGoogle Scholar
Delbés, C, Ali-Mandjee, L & Montel, MC 2007 Monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16S rRNA gene-based analyses. Applied Environmental Microbiology 73 1882–1891CrossRefGoogle ScholarPubMed
Fadda, ME, Mossa, V, Pisano, MB, Deplano, M & Cosentino, S 2004 Occurrence and characterization of yeasts isolated from artisanal Fiore Sardo cheese. International Journal of Food Microbiology 95 51–59Google Scholar
Fatichenti, F, Bergere, JL, Deiana, P & Farris, GA 1983 Antagonistic activity of Debaryomyces hansenii towards Clostridium tyrobutyricum and Clostridium butyricum. Journal of Dairy Research 50 449–457Google Scholar
Fleet, GH 2003 Yeast interactions and wine flavour. International Journal of Food Microbiology 86 11–22Google Scholar
Fonseca, GG, Heinzle, E, Wittmann, C & Gombert, AK 2008 The yeast Kluyveromyces marxianus and its biotechnological potential. Applied Microbiology and Biotechnology 79 339–354Google Scholar
Gardini, F, Tofalo, R, Belletti, N, Iucci, L, Suzzi, G, Torriani, S, Guerzoni, ME & Lanciotti, R 2006 Characterization of yeasts involved in the ripening of Pecorino Crotonese cheese. Food Microbiology 23 641–648Google Scholar
Giannino, ML, Marzotto, M, Dellaglio, F & Feligini, M 2009 Study of microbial diversity in raw milk and fresh curd used for Fontina cheese production by culture-independent methods. International Journal of Food Microbiology 130 188–195CrossRefGoogle ScholarPubMed
Giannino, ML, Buffoni, JN, Massone, E & Feligini, M 2011 Internal transcribed spacer as a target to assess yeast biodiversity in Italian Taleggio PDO cheese. Journal of Food Science 76 511–514CrossRefGoogle ScholarPubMed
Goerges, S, Aigner, U, Silakowski, B & Scherer, S 2006 Inhibition of Listeria monocytogenes by food-borne yeasts. Applied Environmental Microbiology 72 313–318Google Scholar
Gomes-Figueiredo, J, Pimentel, IC, Vicente, VA, Pie, MR, Kava-Cordeiro, V, Galli-Terasawa, L, Pereira, JO, de Souza, AQ & Glienke, C 2007 Bioprospecting highly diverse endophytic Pestalotiopsis spp. with antibacterial properties from Maytenus ilicifolia, a medicinal plant from Brazil. Canadian Journal of Microbiology 53 1123–1132Google Scholar
Hall, TA 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41 95–98Google Scholar
Hocking, AD & Faedo, M 1992 Fungi causing thread mould spoilage of vacuum packaged Cheddar cheese during maturation. International Journal of Food Microbiology 16 123–130Google Scholar
Kamada, R, Monden, Y, Uehara, K, Yamakawa, R & Nishimura, K 2012 Rare case of fungal keratitis caused by Plectosporium tabacinum. Journal of Clinical Ophthalmology 6 1623–1627CrossRefGoogle ScholarPubMed
Kurtzman, CP & Robnett, CJ 1997 Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. Journal of Clinical Microbiology 35 1216–1223CrossRefGoogle ScholarPubMed
Lopandic, K, Zelger, S, Banszky, LK, Eliskases-Lechner, F & Prillinger, H 2006 Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiology 23 341–350Google Scholar
Lucia, V, Daniela, B & Rosalba, L 2001 Use of Fourier transform infrared spectroscopy to evaluate the proteolytic activity of Yarrowia lipolytica and its contribution to cheese ripening. International Journal of Food Microbiology 69 113–123Google Scholar
Maharachchikumbura, SSN, Liang-Dong, G, Ekachai, C, Bahkali, AH & Hyde, KD 2011 Pestalotiopsis – morphology, phylogeny, biochemistry and diversity. Fungal Diversity 50 167–187Google Scholar
McMullen, M, Jones, R & Gallenberg, D 1997 Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Disease 81 1340–1348Google Scholar
Millner, PD 2009 Bioaerosols associated with animal production operations. Bioresource Technology 100 5379–5385Google Scholar
Mounier, J, Monnet, C, Vallaeys, T, Arditi, R, Sarthou, AS, Hélias, A & Irlinger, F 2008 Microbial interactions within a cheese microbial community. Applied Environmental Microbiology 74 172–181Google Scholar
Oliver, SP, Jayarao, BM & Almeida, RA 2005 Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathogens and Disease 2 115–129Google Scholar
Panelli, S, Buffoni, JN, Bonacina, C & Feligini, M 2012 Identification of moulds from the Taleggio cheese environment by the use of DNA barcodes. Food Control 28 385–391CrossRefGoogle Scholar
Pereira-Dias, S, Potes, ME, Marinho, A, Malfeito-Ferreira, M & Loureiro, V 2000 Characterisation of yeast flora isolated from an artisanal Portuguese ewes’ cheese. Internationl Journal of Food Microbiology 60 55–63Google Scholar
Pirisi, A, Comuniana, R, Urgegheb, PP & Scintua, MF 2011 Sheep's and goat's dairy products in Italy: technological, chemical, microbiological, and sensory aspects. Small Ruminant Research 101 102–112Google Scholar
de Souza Leite, T, Cnossen-Fassoni, A, Pereira, OL, Mizubuti, ES, de Araújo, EF & de Queiroz, MV 2013 Novel and highly diverse fungal endophytes in soybean revealed by the consortium of two different techniques. Journal of Microbiology 1 56–69Google Scholar
Zhang, XY, Zhang, Y, Xu, XY & Qi, SH 2013 Diverse deep-sea fungi from the South China sea and their antimicrobial activity. Current Microbiology 67 525–530Google Scholar