Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-20T11:48:10.274Z Has data issue: false hasContentIssue false
  • English
  • Français

Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents

Published online by Cambridge University Press:  01 June 2009

Tuula Honkenen-Buzalski  and
Markus Sandholm
Tuula Honkenen-Buzalski
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland
Markus Sandholm
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland
Get access Rights & Permissions [Opens in a new window]

Summary

The trypsin-inhibitor capacity of bovine milk was seen to increase in mastitits. This capacity showed good correlation with the California Mastitis Test score, somatic cell count (Coulter Counter method) and bovine serum albumin (BSA) content (radial immunodiffusion). The elevated antitrypsin level proved to be a safer indicator of mastitis than BSA alone. Using gel-filtration chromatography, the major trypsin-inhibitor activity of mastitic milk and plasma was eluted with BSA, indicating a similar mol. wt (70000). This fraction showed electrophoretic polymorphism. A large molecular weight fraction cross-reacted immunologically with human α2-macroglobulin. The antitrypsin activity in mastitic milk was clearly different from the principal activity in colostrum, which had a mol. wt of about 13000.

Type
Original Articles
Information
Journal of Dairy Research , Volume 48 , Issue 2 , June 1981 , pp. 213 - 223
Copyright
Copyright © Proprietors of Journal of Dairy Research 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Blombäck, B. & Hanson, L. Å. (1979). Plasma Proteins, pp. 343–344. New York: Wiley.Google Scholar
Butler, J. E. (1971). Journal of Dairy Science 54, 1315–1316.CrossRefGoogle Scholar
Čechová, D. (1976). Methods in Enzymology 45, 806–813.CrossRefGoogle Scholar
Ganrot, P. O., Laurell, C-B. & Eriksson, S. (1967) Scandinavian Journal of Clinical and Laboratory Investigation 19, 205–208.CrossRefGoogle Scholar
Heimburger, N. (1975). In Proteases and Biological Control, pp. 367–386 (Ed. Reich, E. et al. ) Cold Spring Harbor Laboratory.Google Scholar
Humbert, G. & Alais, C. (1979). Journal of Dairy Research 46, 559–571.CrossRefGoogle Scholar
Klastrup, O. & Schmidt Madsen, P. (1974). Nordisk Veterinaermedicin 26, 197–204.Google Scholar
Laskowski, M. Jr & Laskowski, M. (1951). Journal of Biological Chemistry 190, 563–573.CrossRefGoogle Scholar
Law, B. A. (1979). Journal of Dairy Research 46, 573–588.CrossRefGoogle Scholar
Mancini, G., Carbonara, A. O. & Here.MANS, J. F. (1965). Immunochemistry 2, 235–254.CrossRefGoogle Scholar
Mittman, C. (1972). Pulmonary Emphysema and Proteolysis. p. 1. New York/London: Academic Press.Google Scholar
Paape, M. J., Wergin, W. P., Guidry, A. J. & Pearson, R. E. (1979). Journal of Dairy Science 62, 135–153.CrossRefGoogle Scholar
Piñeiro, A., Orteoa, F. & Uriel, J. (1975). Biochimica et Biophysica Acta 379, 201–206.CrossRefGoogle Scholar
Sandholm, M. & Honkanen-Buzalski, T. (1979). Acta Veterinaria Scandinavica 20, 469–476.CrossRefGoogle Scholar
Schalm, O. W. (1977). Journal of the American Veterinary Medical Association 170, 1137–1140.Google Scholar
Schalm, O. W., Carroll, E. J. & Jain, N. C. (1971). Bovine Mastitis, p. 1. Philadelphia, Pa: Lea & Febiger.Google Scholar
Smith, A. M., Chesworth, J. M., Henderson, G. D. & Rodway, R. G. (1979). Journal of Dairy Research 46, 547–550.CrossRefGoogle Scholar
Tolle, A., Zeidler, H. & Heeschen, W. (1966). Milchwissenschaft 21, 93–98.Google Scholar
Travis, J., Baugh, R., Giles, P. J., Johnson, D., Bower, J. & Reilly, C. F. (1978). Neutral Proteases of Human Polymorphonuclear Leukocytes (Ed. Haveman, K. and Janoff., A.) pp. 118–128, Baltimore & Munich: Urban & Schwarzenberg, Inc.Google Scholar
Von Fellenbero, R. & Horber, H. (1980). Schweizer Archiv für Tierheilkunde 122, 159–168.Google Scholar
Weeke, B. (1973). Scandinavian Journal of Immunology 2, Suppl. 1, 15–36.CrossRefGoogle Scholar
Weissmann, G., Korchak, H. E., Perez, H. D., Smolen, J. E., Goldstein, I. M. & Hoffstein, S. T. (1979). Advances in Inflammation Research 1, pp. 95–112, New York: Raven Press.Google Scholar