Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-26T10:23:59.070Z Has data issue: false hasContentIssue false

The status of casein micelles in chilled milk from the buffalo* and the cow

Published online by Cambridge University Press:  01 June 2009

Pershotam Kumar Sabarwal
Affiliation:
National Dairy Research Institute, Karnal, Haryana, India‡
N. C. Ganguli
Affiliation:
National Dairy Research Institute, Karnal, Haryana, India‡

Summary

When milk was chilled for 12 and 24 h at 0 °C, a decrease in the calcium and phosphorus contents of the casein micelle of both buffalo and cow's milk was observed. This decrease was directly proportional to the cooling period. A proportionate increase with cooling in the sialic-acid content of the casein micelle of chilled milk was noticed. Casein micelle isolated from chilled milk exhibited low opacity compared with the normal counterpart.

A decrease in micellar casein and an increase in soluble casein were observed in both buffalo and cow's milk, in proportion to the cooling period. Similar results were obtained when casein micelles were resuspended in milk dialysate and centrifuged.

Casein micelles from chilled milk showed different patterns on starch-gel electrophoresis compared with normal micelles. The intensity of the κ-casein band was relatively higher in the chilled samples. Gel-filtration studies suggest that some molecular rearrangement occurs in the casein micelle when milk is chilled.

The release of sialopeptide from micellar casein by rennet was not influenced by chilling. However, a decrease in the rate of turbidity development in renneted suspensions of casein micelles was noted in chilled milk, which suggests a change in the structure of the casein micelle.

No difference was noticed between buffalo and cow's milk in these respects.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Burton, H. (1956). Journal of Dairy Research 23, 92.CrossRefGoogle Scholar
Carroll, R. J., Thompson, M. P., Brunner, J. R. & Kolar, C. (1967). Journal of Dairy Science 50, 941.Google Scholar
Chen, C. & Yamauchi, K. (1969 a). Agricultural and Biological Chemistry 33, 1333.CrossRefGoogle Scholar
Chen, C. & Yamauchi, K. (1969 b). Agricultural and Biological Chemistry 33, 1751.CrossRefGoogle Scholar
Davies, D. T. & White, J. C. D. (1962). Journal of Dairy Research 29, 285.CrossRefGoogle Scholar
Fiske, C. H. & Subbarow, Y. (1925). Journal of Biological Chemistry 66, 375.CrossRefGoogle Scholar
Ganguli, N. C. (1968). Indian Journal of Veterinary Science and Animal Husbandry 38, 1.Google Scholar
Ganguli, N. C. & Majumder, G. C. (1967). Indian Journal of Biochemistry 4, 232.Google Scholar
Green, M. L. & Crutchfield, G. (1971). Journal of Dairy Research 38, 151.CrossRefGoogle Scholar
Gupta, S. K. & Ganguli, N. C. (1965). Indian Journal of Biochemistry 2, 253.Google Scholar
Gupta, S. K. & Ganguli, N. C. (1967). Indian Journal of Biochemistry 4, 47.Google Scholar
Kadan, R. S. (1967). Dissertation Abstracts B 28, 732.Google Scholar
Kolar, C. W. Jr & Brunner, J. R. (1967). Journal of Dairy Science 50, 941.Google Scholar
Laxminarayana, H. & Dastur, N. N. (1968). Dairy Science Abstracts 30, 177, 231.Google Scholar
Lindqvist, B. (1963). Dairy Science Abstracts 25, 257, 299.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Journal of Biological Chemistry 193, 265.CrossRefGoogle Scholar
Rose, D. (1968). Journal of Dairy Science 51, 1897.CrossRefGoogle Scholar
Rose, D. & Colvin, J. R. (1966). Journal of Dairy Science 49, 1091.CrossRefGoogle Scholar
Sabarwal, P. K. & Ganguli, N. C. (1968). Milchwissenschaft 23, 686.Google Scholar
Sabarwal, P. K. & Ganguli, N. C. (1969). Indian Journal of Dairy Science 22, 128.Google Scholar
Sabarwal, P. K. & Ganguli, N. C. (1970). Indian Journal of Dairy Science 23, 24.Google Scholar
Sabarwal, P. K. & Ganguli, N. C. (1971). Indian Journal of Dairy Science 24, 16.Google Scholar
Saito, Z., Niki, R. & Hashimoto, Y. (1963). Journal of Agriculture, Hokkaido University 53, 200.Google Scholar
Sullivan, R. A., Fitzpatrick, M. M., Stanton, E. K., Annino, R., Kissel, G. & Palermiti, F. (1955). Archives of Biochemistry and Biophysics 55, 455.CrossRefGoogle Scholar
Warren, L. (1959). Journal of Biological Chemistry 234, 1971.CrossRefGoogle Scholar
Winder, W. C. (1962). Journal of Dairy Science 45, 1024.CrossRefGoogle Scholar
Yaguchi, M. & Tarassuk, N. P. (1965). Journal of Dairy Science 48, 773.Google Scholar
Yamauchi, K. & Chen, C. (1969). Agricultural and Biological Chemistry 23, 1761.CrossRefGoogle Scholar
Yamauchi, K., Chen, C. & Tsugo, T. (1967). Agricultural and Biological Chemistry 31, 581.Google Scholar
Yoshida, S. (1966). Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University 6, 499.Google Scholar
Yoshida, S. (1967). Journal of the Agricultural Chemical Society of Japan 41, 359.Google Scholar