Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T09:46:09.423Z Has data issue: false hasContentIssue false
  • English
  • Français

Simple methods for the purification of crude κ-casein and β-casein by treatment with calcium phosphate gel

Published online by Cambridge University Press:  01 June 2009

Margaret L. Green
Margaret L. Green
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
Get access Rights & Permissions [Opens in a new window]

Summary

Batch methods applicable on a large scale are described for the purification of crude κ- and βκ-Casein, dissolved in urea-containing buffer, was freed from αs- and β-caseins by treatment with calcium phosphate gel and recovered in about 60% yield. β-Casein was freed from most impurities by adsorption on to calcium phosphate gel at pH 7·8 in the presence of urea and elution with 6 M-urea–N-NH4OH at 4°C. The recovery was about 50%.

Type
Original Articles
Information
Journal of Dairy Research , Volume 36 , Issue 3 , October 1969 , pp. 353 - 359
Copyright
Copyright © Proprietors of Journal of Dairy Research 1969

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aschaffenburg, R. (1963). J. Dairy Res. 30, 259.CrossRefGoogle Scholar
Cheeseman, G. C. (1968). J. Dairy Res. 35, 439.Google Scholar
Colowick, S. P. (1955). Meth. Enzym. 1, 98.Google Scholar
Garnier, J., Ribadeau-Dumas, B. & Mocquot, G. (1964). J. Dairy Res. 31, 131.CrossRefGoogle Scholar
Gehrke, C. W., Freeark, C. W., Oh, Y. H. & Chun, P. W. (1964). Analyt. Biochem. 9, 423.CrossRefGoogle Scholar
Groves, M. L., McMeekin, T. L., Hipp, N. J. & Gordon, W. G. (1962). Biochim. biophys. Acta 57, 197.CrossRefGoogle Scholar
Hill, R. D. (1963). J. Dairy Res. 30, 101.Google Scholar
Thompson, M. P. (1966). J. Dairy Sci. 49, 792.CrossRefGoogle Scholar
Waugh, D. F. (1961). J. phys. Chem., Ithaca 65, 1793.CrossRefGoogle Scholar
Yaguchi, M., Davies, D. T. & Kim, Y. K. (1968). J. Dairy Sci. 51, 473.CrossRefGoogle Scholar
Zittle, C. A. (1961). J. Dairy Sci. 44, 2101.Google Scholar
Zittle, C. A., Cerbulis, J., Pepper, L. & Della Monica, E. S. (1959). J. Dairy Sci. 42, 1897.CrossRefGoogle Scholar
Zittle, C. A. & Custer, J. H. (1963). J. Dairy Sci. 46, 1183.CrossRefGoogle Scholar
Zittle, C. A. & Walter, M. (1963). J. Dairy Sci. 46, 1189.CrossRefGoogle Scholar