Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-27T00:11:28.071Z Has data issue: false hasContentIssue false
  • English
  • Français

Seasonal variation and characterization of Micrococcaceae present in ewes' raw milk

Published online by Cambridge University Press:  01 June 2009

Lydia Bautista ,
Monserrat P. Bermejo  and
Manuel Nuñez
Lydia Bautista
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28040, Spain
Monserrat P. Bermejo
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28040, Spain
Manuel Nuñez
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28040, Spain
Get access Rights & Permissions [Opens in a new window]

Summary

Samples (120) of ewes' raw milk collected over a 12-month period on arrival at the dairy and freshly drawn ewes’ milk samples (30) collected at the farm were analysed for total viable counts and staphylococci. Total viable counts reached on the average 54 × 106/ml in dairy samples and 6·0 × 105/ml in farm samples, whereas mean levels of staphylococci were 5·9 × 104/ml and 3·3 × 103/ml, respectively. A high proportion (62%) of coagulase-positive isolates from Baird-Parker agar plates was detected in spring months. Staphylococcus aureus (42%), Staph. haemolyticus (23%) and Staph. epidermidis (10%) were the predominant species in 210 characterized isolates of staphylococci from ewes' raw milk. Seven coagulase-positive, maltose-negative staphylococci could not be identified with any of the recognized Staphylococcus species. Eighteen Micrococcus strains were identified as M. varians, whereas 111 Micrococcus strains of uncertain taxonomic status were separated into three groups, according to their physiological characters.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 1 , February 1986 , pp. 1 - 5
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abd-El-Malek, Y. & Gibson, T. 1948 Studies in the bacteriology of milk. II. The staphylococci and micrococci of milk. Journal of Dairy Research 15 249–260CrossRefGoogle Scholar
Baird-Parker, A. C. 1979 Methods for identifying staphylococci and micrococci. In Identification Methods for Microbiologists pp. 201–210 (Eds Skinner, F. A. and Lovelock, D. W.). London: Academic Press. (Society for Applied Bacteriology Technical Series no. 14)Google Scholar
Casman, E. P. 1965 Staphylococcal enterotoxin. Annals of the New York Academy of Sciences 128 124–131.CrossRefGoogle ScholarPubMed
Cousins, C. M. & Bramley, A. J. 1981 The microbiology of raw milk. In Dairy Microbiology pp. 119–163 (Ed. Robinson, R. K.). London: Applied Science Publishers LtdGoogle Scholar
Devriese, L. A. & De Keyser, H. 1980 Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows. Journal of Dairy Research 47 155–158CrossRefGoogle ScholarPubMed
Dodd, F. H. & Neave, F. K. 1970 Mastitis control. Biennial Review, National Institute for Research in Dairying pp. 21–60Google Scholar
Dos Santos, E. C., Genigeorgis, C. & Farver, T. B. 1981 Prevalence of Staphylococcus aureus in raw and pasteurized milk used for commercial manufacturing of Brazilian Minas cheese. Journal of Food Protection 44 172–176CrossRefGoogle Scholar
Gaya, P., Medina, M., Bautista, L. & Nuñez, M. 1984 Survival of Staphylococcus aureus in raw sheep milk Manchego cheeses ripened at different temperatures. Journal of Applied Bacteriology 57 xviiGoogle Scholar
Gehringer, G. 1981 Multiplication of bacteria in milk during farm storage. Kieler Milchwirtschaftliche Forschmigsberichte 33 317–323Google Scholar
Gutierrez, L. M., Menes, I., Garcia, M., Moreno, B. & Bergdoll, M. S. 1982 Characterization and enterotoxigenicity of staphylococci isolated from mastitic ovine milk in Spain. Journal of Food Protection 45 1282–1286CrossRefGoogle Scholar
Hájek, V. 1978 Identification of enterotoxigenic staphylococci from sheep and sheep cheese. Applied and Environmental Microbiology 35 264–268CrossRefGoogle ScholarPubMed
Kloos, W. E. & Schleifer, K. H. 1975 Simplified scheme for routine identification of human Staphylococcus species. Journal of Clinical Microbiology 1 82–88CrossRefGoogle ScholarPubMed
Kloos, W. E., Tornabene, T. G. & Schleifer, K. H. 1974 Isolation of micrococci from human skin including two new species: Micrococcus lylae and Micrococcus kristinae. International Journal of Systematic Bacteriology 24 79–101CrossRefGoogle Scholar
Kocur, M., Pacova, Z. & Martinec, T. 1972 Taxonomic status of Micrococcus luteus (Schroeter 1872) Cohn 1872, and designation of the neotype strain. International Journal of Systematic Bacteriology 22 218–223CrossRefGoogle Scholar
Nuñez, J. A., Chavarri, F.J. & Nuñez, M. 1984 Psychrotrophic bacterial flora of ewes' raw milk, with particular reference to Gram negative rods. Journal of Applied Bacteriology 57 23–29CrossRefGoogle ScholarPubMed
Olson, J. C., Casman, E. P., Baer, E. F. & Stove, J. E. 1970 Enterotoxigenicity of Staphylococcus aureus cultures isolated from acute cases of bovine mastitis. Applied Microbiology 20 605–607CrossRefGoogle ScholarPubMed
Sharpe, M. E., Fewins, B. G., Reiter, B. & Cuthbert, W. A. 1965 A survey of the incidence of coagulase-positive staphylococci in market milk and cheese in England and Wales. Journal of Dairy Research 32 187–192CrossRefGoogle Scholar
Sharpe, M. E., Neave, F. K. & Reiter, B. 1962 Staphylococci and micrococci associated with dairying. Journal of Applied Bacteriology 25 403–415CrossRefGoogle Scholar
Tatini, S. R., Jezeski, J. J., Morris, H. A., Olson, J. C. & Casman, E. P. 1971 Production of staphylococcal enterotoxin A in Cheddar and Colby cheeses. Journal of Dairy Science 54 815–825CrossRefGoogle Scholar
Tatini, S. R., Wesala, W. D., Jezeski, J. J. & Morris, H. A. 1973 Production of staphylococcal enterotoxin A in Blue, Brick, Mozzarella, and Swiss cheese. Journal of Dairy Science 56 429–435CrossRefGoogle Scholar
Waes, G. 1977 [Presence of coagulase-positive staphylococci in Hervé cheese.] Revue del' Agriculture 30 617–628Google Scholar
Zehren, V. L. & Zehren, V. F. 1968 Examination of large quantities of cheese for staphylococcal enterotoxin A. Journal of Dairy Science 51 635–644CrossRefGoogle Scholar