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Sandwich enzyme immunoassay of Mucor miehei proteinase (Fromase) in cheese

Published online by Cambridge University Press:  01 June 2009

Pavel Rauch ,
Igor Hochel ,
Eva Beránková  and
Jan Káš
Pavel Rauch
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Igor Hochel
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Eva Beránková
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Jan Káš
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
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Summary

Sandwich enzyme immunoassay of Mucor miehei proteinase (from the commercial preparation Fromase) has been developed using horseradish peroxidase as a marker. The enzyme may be determined to a detection limit of 50 ng/ml. Slight cross reactions by chymosin, bovine, porcine and chicken pepsin did not affect the assay. Recovery of Fromase added to cheese extracts varied between 92 and 106%. The method was used for the determination of M. miehei proteinase in different cheese extracts.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 793 - 797
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

REFERENCES

Beránková, E., Rauch, P., Káš, J., Hušek, V. & Paluska, E. 1987 Indirect assay of milk clotting activity of Mucor miehei proteinase (Fromase) in combined rennets. Journal of Dairy Research 56 407–412CrossRefGoogle Scholar
Britton, H. T. S. 1955 Hydrogen Ions, 4th edn, Vol. 1, p. 369. London: Chapman & HallGoogle Scholar
Darley, R. L., Morris, B. A., Clifford, M. N. & Gould, B. J. 1988 Detection of enzymes used as food additives by immunoassay as exemplified by an ELISA for Mucor miehei rennet in cheese. In Immunoassays for Veterinary and Food Analysis-1, pp. 363–367 (Eds Morris, B. A., Clifford, M. N. and Jackman, R.) London: ElsevierGoogle Scholar
Daussant, J. & Bureau, D. 1984 Immunochemical methods in food analysis. In Developments in Food Analysis Techniques-3 pp. 175–210 (Ed. King, R. D.) London: ElsevierGoogle Scholar
Donhauser, S. 1979 [Radioimmunoassay for the detection of bromelain and ficin in beer.] Brauwissenschaft 32 211–213Google Scholar
Elliott, J. A. & Emmons, D. B. 1971 Rennin detection in cheese with the passive indirect hemagglutination test. Canadian Institute of Food Technology Journal 4 16–18CrossRefGoogle Scholar
Guesdon, J.-L. 1988 Amplification systems for enzyme immunoassay. In Nonisotopic Immunoassay pp. 85–106 (Ed. Ngo, T. T.) New York: Plenum PressCrossRefGoogle Scholar
Harboe, M. K. 1985 Commercial aspects of aspartic proteases. In Aspartic Proteinases and their Inhibitors pp. 537–549 (Ed. Kostka, V.) Berlin: W. de GruyterGoogle Scholar
Holmes, D. G., Duersch, J. W. & Ernstrom, C. A. 1977 Distribution of milk clotting enzymes between curd and whey and their survival during Cheddar cheese making. Journal of Dairy Science 60 862–869CrossRefGoogle Scholar
Majeed, G. H. & Ernstrom, C. A. 1985 Estimation of residual pepsin and chymosin activity in curd. Journal of Dairy Science 68 1936–1938CrossRefGoogle Scholar
Matheson, A. R. 1981 The immunochemical determination of chymosin activity in cheese. New Zealand Journal of Dairy Science and Technology 16 33–41Google Scholar
Morris, B. A. & Clifford, M. N. 1985 Immunoassays in Food Analysis. London: ElsevierGoogle Scholar
Stadhouders, J., Hup, G. & Van Der Waals, C. B. 1977 Determination of calf rennet in cheese. Netherlands Milk and Dairy Journal 31 3–15Google Scholar
Vaag, P. & Munck, L. 1987 Immunochemical methods in cereal research and technology. Cereal Chemistry 64 59–72Google Scholar
Wilson, M. B. & Nakane, P. K. 1978 Recent developments in the periodate method of conjugating horseradish peroxidase to antibodies. In Immunofluorescence and Related Staining Techniques pp. 215–224 (Eds Knapp, W., Holubar, K. and Wick, G.) Amsterdam: ElsevierGoogle Scholar