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Salt distribution between the colloidal and soluble phases of ewes' milk

Published online by Cambridge University Press:  01 June 2009

Anna Polychroniadou
Affiliation:
Laboratory of Dairy Technology, Faculty of Agriculture, University of Thessaloniki, Thessaloniki, Greece
Anna Vafopoulou
Affiliation:
Laboratory of Dairy Technology, Faculty of Agriculture, University of Thessaloniki, Thessaloniki, Greece

Summary

Ewes' milk from two breeds of Greek sheep, Karagouniki and Serron, was analysed throughout mid and late lactation to investigate the salt distribution between colloidal and soluble phases. Separation of the phases was obtained by centrifugation at 48000 g. Effect of stage of lactation was significant only for total Mg in both breeds (P < 0·05) and for colloidal citrate in Serron ewe milk (P < 0·01). Breed differences were not significant (P < 0·05), except for colloidal casein.

In the colloidal phase there was a strong positive correlation of casein with Ca and P, but correlation with Mg was poor. Molar sums of [Ca + Mg] and [P + Cit] were also significantly correlated. The Ca to P molar ratio averaged 2·00 and was higher than that reported for cows' milk. The inclusion of Mg as well as citrate in the colloidal calcium phosphate is indicated. In the aqueous phase there was a strong negative correlation of Ca with pH and a positive one with citrate. Correlation with phosphate was less. It seems that citrate is the main factor influencing Ca concentration in the soluble phase.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

REFERENCES

Alais, C. & Jollés, P. 1961 [Comparative study of the casein-glycopeptides obtained after rennin digestion of the caseins of the milk of cow, sheep and goat. II. Study of the non-peptide part.] Biochimica et Biophysica Acta 51 315322CrossRefGoogle Scholar
Ali, A. E., Andrews, A. T. & Cheeseman, G. C. 1980 Factors influencing casein distribution in cold-stored milk and their effect on cheesemaking parameters. Journal of Dairy Research 47 383391CrossRefGoogle Scholar
Brulé, G., Real Del Sol, E., Fauquant, J. & Fiaud, C. 1978 Mineral salts stability in aqueous phase of milk: influence of heat treatments. Journal of Dairy Science 61 12251232CrossRefGoogle Scholar
Chaplin, L. C. 1984 Studies on micellar calcium phosphate: composition and apparent solubility product in milk over a wide pH range. Journal of Dairy Research 51 251257CrossRefGoogle Scholar
Davies, D. T. & White, J. C. 1960 The use of ultrafiltration and dialysis in isolating the aqueous phase of milk and in determining the partition of milk constituents between the aqueous and disperse phases. Journal of Dairy Research 27 171190CrossRefGoogle Scholar
Dickson, I. R. & Perkins, D. J. 1971 Studies on the interactions between purified bovine caseins and alkaline-earth-metal ions. Biochemical Journal 124 235240CrossRefGoogle ScholarPubMed
Faulkner, A. & Peaker, M. 1982 Reviews of the progress of dairy science: Secretion of citrate into milk. Journal of Dairy Research 49 159169CrossRefGoogle ScholarPubMed
Fox, P. F. & Mulvihill, D. M. 1982 Milk proteins: molecular, colloidal and functional properties. Journal of Dairy Research 49 679693CrossRefGoogle Scholar
Grandison, A. S., Ford, G. D., Owen, A. J. & Millard, D. 1984 Chemical composition and coagulating properties of renneted Friesian milk during the transition from winter rations to spring grazing. Journal of Dairy Research 51 6978CrossRefGoogle Scholar
Holt, C. 1982 Inorganic constituents of milk. III. The colloidal calcium phosphate of cow's milk. Journal of Dairy Research 49 2938CrossRefGoogle ScholarPubMed
Holt, C. & Jenness, R. 1984 Interrelationships of constituents and partition of salts in milk samples from eight species. Comparative Biochemistry and Physiology 77 A 275282CrossRefGoogle ScholarPubMed
Holt, C. & Muir, D. D. 1979 Inorganic constituents of milk. I. Correlation of soluble calcium with citrate in bovine milk. Journal of Dairy Research 46 433439CrossRefGoogle Scholar
Irlam, J. C., Holt, C., Haskain, S. S. & Hukins, D. W. L. 1985 Comparison of the structure of micellar calcium phosphate in milk from six species by extended X-ray absorption fine structure spectroscopy. Journal of Dairy Research 52 267273CrossRefGoogle Scholar
Lyster, R. L. J. 1979 The equilibria of calcium and phosphate ions with the micellar calcium phosphate in cow's milk. Journal of Dairy Research 46 343346CrossRefGoogle ScholarPubMed
Lyster, R. L. J., Mann, S., Parker, S. B. & Williams, R. J. P. 1984 Nature of micellar calcium phosphate in cows' milk as studied by high-resolution electron microscopy. Biochimica et Biophysica Acta 801 315317CrossRefGoogle ScholarPubMed
McGann, T. C. A., Buchheim, W., Kearney, R. D. & Richardson, T. 1983 Composition and ultrastructure of calcium phosphate-citrate complexes in bovine milk systems. Biochimica et Biophysica Acta 760 415420CrossRefGoogle ScholarPubMed
Mehaia, M. A. & Cheryan, M. 1983 The secondary phase of milk coagulation. Effect of calcium, pH and temperature on clotting activity, Milchwissenschaft 38 137140Google Scholar
O'Connor, P. & Fox, P. F. 1977 The proteins and salts of some non-bovine milks. Journal of Dairy Research 44 607609CrossRefGoogle ScholarPubMed
Pierre, A., Brulè, G. & Fauquant, J. 1983 [Study of calcium exchangeability in milk with 45Ca.] Lait 63 473489CrossRefGoogle Scholar
Polychroniadou, A. & Vafopoulou, A. 1985 Variations of major mineral constituents of ewe milk during lactation. Journal of Dairy Science 68 147150CrossRefGoogle Scholar
Pyne, G. T. 1962 Reviews of the progress of dairy science. Section C. Dairy chemistry. Some aspects of the physical chemistry of the salts of milk. Journal of Dairy Research 29 101130CrossRefGoogle Scholar
Pyne, G. T. & McGann, T. C. A. 1960 The colloidal phosphate of milk. II. Influence of citrate. Journal of Dairy Research 27 917CrossRefGoogle Scholar
Richardson, B. C., Creamer, L. K., Pearce, K. N. & Munford, R. E. 1974 Comparative micelle structure. II. Structure and composition of casein micelles in ovine and caprine milk as compared with those in bovine milk. Journal of Dairy Research 41 239247CrossRefGoogle Scholar
White, J. C. D. & Da Vies, D. T. 1958 The relation between the chemical composition of milk and the stability of the caseinate complex. I. General introduction, description of samples, methods and chemical composition of samples. Journal of Dairy Research 25 236255CrossRefGoogle Scholar