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A rapid HPLC method for the determination of lactoferrin in milk of various species

Published online by Cambridge University Press:  30 April 2019

Efstathia Tsakali ,
Arhontoula Chatzilazarou ,
Dimitra Houhoula ,
Spiridon Koulouris ,
John Tsaknis  and
Jan Van Impe
Efstathia Tsakali*
Affiliation:
Department of Food Science and Technology, University of West Attica, Greece Department of Chemical Engineering, BioTeC+ - Chemical and Biochemical Process Technology and Control, KU Leuven, Belgium
Arhontoula Chatzilazarou
Affiliation:
Department of Wine, Vine and Beverage Sciences, University of West Attica, Greece
Dimitra Houhoula
Affiliation:
Department of Food Science and Technology, University of West Attica, Greece
Spiridon Koulouris
Affiliation:
Department of Food Science and Technology, University of West Attica, Greece
John Tsaknis
Affiliation:
Department of Food Science and Technology, University of West Attica, Greece
Jan Van Impe
Affiliation:
Department of Chemical Engineering, BioTeC+ - Chemical and Biochemical Process Technology and Control, KU Leuven, Belgium
*
Author for correspondence: Efstathia Tsakali, Email: [email protected]
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Abstract

This Research Communication describes the adaptation and testing of an RP-HPLC method, previously tested for the determination of lactoferrin (LF) in whey, for its applicability to determine milk lactoferrin content. Milk samples of various species, namely, ovine, caprine, bovine, donkey and human milk, were tested. The advantage of this RP-HPLC method includes speed and convenience, as it does not include extensive pretreatment or separation steps. A simple pre-treatment step was added in order to remove fat and proteins of the casein family and the samples were tested. The results varied in terms of elution of the LF peak both between the milk of the different species as well as from the initial application on whey. The peak resolution was satisfactory in the cases of ovine, bovine and donkey milk samples while for caprine and human milk an interference with other peaks was observed. Nevertheless, quantification of LF was found possible for all samples. This new application of the modified method will allow the determination of LF in milk samples of the tested species either for everyday analysis or as a useful qualitative screening for presence or absence of LF.

Keywords

LactoferrinHPLC, milkwhey proteinmilk composition
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 238 - 241
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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References

Alexandraki, V and Moatsou, G (2018) Para-κ-casein during the ripening and storage of low-pH, high-moisture Feta cheese. Journal of Dairy Research 85, 226–231.Google Scholar
Cheng, JB, Wang, JQ, Bu, DP, Liu, GL, Zhang, CG, Wei, HY, Zhou, LY and Wang, JZ (2008) Factors affecting the lactoferrin concentration in bovine milk. Journal of Dairy Science 91, 970–976.Google Scholar
Fong, BY, Norris, CS and MacGibbon, AKH (2007) Protein and lipid composition of bovine milk-fat-globule membrane. International Dairy Journal 17, 275–288.Google Scholar
García-Montoya, IA, Cendón, TS, Arévalo-Gallegos, S and Rascón-Cruz, Q (2012) Lactoferrin a multiple bioactive protein: an overview. Biochimica et Biophysica Acta 1820, 226–236.Google Scholar
Gubid, J, Milovanovid, I, Iličid, M, Tomid, J, Torbica, A, Šarid, L and Ilid, N (2015) Comparison of the protein and fatty acid fraction of Balkan donkey and human milk. Mljekarstvo 65, 168–176.Google Scholar
Indyk, HE and Filonzi, EL (2005) Determination of lactoferrin in bovine milk, colostrum and infant formulas by optical biosensor analysis. International Dairy Journal 15, 429–438.Google Scholar
Jenssen, H and Hancock, REW (2009) Antimicrobial properties of lactoferrin. Biochimie 91, 19–29.Google Scholar
Lampreave, F, Pineiro, A, Brock, JH, Castillo, H, Sanchez, L and Calvo, M (1990) Interaction of bovine lactoferrin with other proteins of milk whey. International Journal of Biological Macromolecules 12, 2–5.Google Scholar
Levay, PF and Viljoen, M (1995) Lactoferrin: a general review. Haematologica 80 252–267Google Scholar
Palmano, KP and Elgar, DF (2002) Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase HPLC on polystyrene-divinylbenzene. Journal of Chromatography A 947, 307–311.Google Scholar
Pocheta, S, Arnoulda, C, Debournouxb, P, Flamentb, J, Rolet-Répécauda, O and Beuviera, E (2018) A simple micro-batch ion-exchange resin extraction method coupled with reverse-phase HPLC (MBRE-HPLC) to quantify lactoferrin in raw and heat-treated bovine milk. Food Chemistry 259, 36–45.Google Scholar
Polidori, P and Vincenzetti, S (2012) Protein profile characterization of Donkey milk. In Hurley, W (ed.), Milk Protein. London: InTech, pp. 215–232Google Scholar
Queiroz, VA, Assis, AM and R Júnior Had, C (2013) Protective effect of human lactoferrin in the gastrointestinal tract. Revista paulista de pediatria 31 90–95Google Scholar
Santillo, A, Figliola, L, Ciliberti, MG, Caroprese, M, Marino, R and Albenzio, M (2018) Focusing on fatty acid profile in milk from different species after in vitro digestion. Journal of Dairy Research 85, 257–262.Google Scholar
Tsakali, E, Petrotos, K, Chatzilazarou, A, Stamatopoulos, K, D'Alessandro, AG, Goulas, P, Massouras, T and Van Impe, J (2014) Short communication: determination of lactoferrin in Feta cheese whey with reversed-phase high-performance liquid chromatography. Journal of Dairy Science 97, 4832–4837.Google Scholar
Wakabayashi, HKY and Takase, M (2006) Lactoferrin research, technology and applications. International Dairy Journal 16, 1241–1251.Google Scholar
Welty, FK, Smith, KL and Schanbacher, FL (1976) Lactoferrin concentration during involution of the bovine mammary gland. Journal of Dairy Science 59, 224–231.Google Scholar