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Purification and characterization of bovine milk fibronectin

Published online by Cambridge University Press:  01 June 2009

Thomas N. Sato
Affiliation:
Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan
Masao Hayashi
Affiliation:
Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan

Summary

Fibronectins were isolated from both bovine colostrum and fresh milk, and they were found to be very similar to plasma fibronectin. The concentration of fibronectin in milk decreased post partum: 30 µg/ml after 0 d and 2 µg/ml after 7–851 d. No fibronectin could be detected in any brands of ultra heat treated liquid milk on the market (≤0·006 µg/ml).

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

REFERENCES

Aplin, J. D. & Hughes, R. C. 1982 Complex carbohydrates of the extracellular matrix: structures, interactions and biological roles. Biochimica et Biophysica Acta 694 375418CrossRefGoogle ScholarPubMed
Ashwell, G. 1966 New colorimetric methods of sugar analysis. Methods in Enzymology 8 8595CrossRefGoogle Scholar
Chandrasekhar, S., Sorrentino, J. A. & Millis, A. J. T. 1983 Interaction of fibronectin with collagen: age-specific defect in the biological activity of human fibroblast fibronectin. Proceedings of the National Academy of Sciences of the USA 80, 47474751CrossRefGoogle ScholarPubMed
Engvall, E. 1980 Enzyme immunoassay ELISA and EMIT. Methods in Enzymology 70 419439CrossRefGoogle ScholarPubMed
Fujikawa, K., Thompson, A. R., Legaz, M. E., Meyer, R. G. & Davie, E. W. 1973 Isolation and characterization of bovine factor IX (Christmas factor). Biochemistry 12 49384945CrossRefGoogle ScholarPubMed
Grinnell, F., Hays, D. G. & Minter, D. 1977 Cell adhesion and spreading factor. Partial purification and properties. Experimental Cell Research 110 175190CrossRefGoogle Scholar
Hahn, L. H. E. & Yamada, K. M. 1979 Identification and isolation of a collagen-binding fragment of the adhesive glycoprotein fibronectin. Proceedings of the National Academy of Sciences of the U.S.A. 76 11601163CrossRefGoogle ScholarPubMed
Hayashi, M. & Hirano, H. 1983 [Structure of fibronectin molecule and gene.] Tanpahishitsu Kakusan Koso 28 169181Google ScholarPubMed
Hayashi, M. & Yamada, K. M. 1981 Differences in domain structures between plasma and cellular fibronectins. Journal of Biological Chemistry 256 1129211300CrossRefGoogle ScholarPubMed
Hayashi, M. & Yamada, K. M. 1982 Divalent cation modulation of fibronectin binding to heparin and to DNA. Journal of Biological Chemistry 257 52635267CrossRefGoogle ScholarPubMed
Hynes, R. O. 1981 Fibronectin and its relation to cellular structure and behaviour. In Cell Biology of the Extracellular Matrix pp. 295334 (Ed. Hay, E. D.). New York: Plenum PressCrossRefGoogle Scholar
Isemura, M., Yamaruohi, Y., Munakata, H., Aikawa, J., Kan, M., Yamane, I. & Yosizawa, Z. 1984 Isolation and characterization of human placenta fibronectin. Journal of Biochemistry 96 163169CrossRefGoogle ScholarPubMed
Ruoslahti, E., Engvall, E., Hayman, E. G. & Spiho, R. G. 1981 Comparative studies on amniotic fluid and plasma fibronectins. Biochemical Journal 193 295299CrossRefGoogle ScholarPubMed
Ruoslahti, E., Hayman, E. G., Pierschbacher, M. & Enovall, E. 1982 Fibronectin: purification, immunochemical properties, and biological activities. Methods in Enzymology 82 803831CrossRefGoogle ScholarPubMed
Saba, T. M. & Jaffe, E. 1980 Plasma fibronectin (opsonic glycoprotein): its synthesis by vascular endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function. American Journal of Medicine 68 577594CrossRefGoogle ScholarPubMed
Steimer, K. S., Packard, R., Holden, D. & Klagsbrun, M. 1981 The serum-free growth of cultured cells in bovine colostrum and in milk obtained later in the lactation period. Journal of Cellular Physiology 109 223234CrossRefGoogle ScholarPubMed
Yamada, K. M. 1983 Cell surface interactions with extracellular materials. Annual Review of Biochemistry 52 761799CrossRefGoogle ScholarPubMed
Yamada, K. M. & Kennedy, D. W. 1979 Fibroblast cellular and plasma fibronectins are similar but not identical. Journal of Cell Biology 80 492498CrossRefGoogle Scholar
Zardi, L., Destree, A., Balza, E. & Isliker, H. 1982 Human milk fibronectin: identification of fibronectin fragments by transfer of milk proteins from polyacrylamide gels to nitrocellulose sheets. FEBS Letters 143 105108CrossRefGoogle ScholarPubMed
Zhu, B. C. R., Fisher, S. F., Pande, H., Calaycay, J., Shively, J. E. & Laine, R. A. 1984 Human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. Journal of Biological Chemistry 259 39623970CrossRefGoogle ScholarPubMed