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Purification and characterization of a cell wall proteinase from Streptococcus lactis NCDO 763

Published online by Cambridge University Press:  01 June 2009

Véronique Monnet ,
Dominique Le Bars  and
Jean-Claude Gripon
Véronique Monnet
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Dominique Le Bars
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Jean-Claude Gripon
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
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Summary

A proteinase was purified from a cell wall extract of a culture of Streptococcus lactis NCDO 763 grown in skim milk. Being active at a low pH (at pH 4·8 on haemoglobin and pH 6·–6·5 on casein) and completely inhibited by diisopropylfluorophosphate, it was considered to be a serine proteinase partly inhibited by EDTA; the mol. wt was ∼ 80000.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 2 , May 1987 , pp. 247 - 255
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

REFERENCES

Bradford, M. M. 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72 248–254CrossRefGoogle ScholarPubMed
Cliffe, A. J. & Law, B. A. 1985 Discontinuous polyacrylamide gel electrophoresis of cell wall proteinases from variants of Streptococcus lactis. Journal of Applied Bacteriology 58 245–250CrossRefGoogle Scholar
Donnelly, W. J., Barry, J. G. & Richardson, T. 1980 14C-methylated β-casein as a substrate for plasmin, and its application to the study of milk protein transformations. Biochimica et Biophysica Acta 626 117–126CrossRefGoogle Scholar
Exterkate, F. A. 1975 An introductory study of the proteolytic system of Streptococcus cremoris strain HP. Netherlands Milk and Dairy Journal 29 303–318Google Scholar
Exterkate, F. A. 1976 Comparison of strains of Streptococcus cremoris for proteolytic activities associated with the cell wall. Netherlands Milk and Dairy Journal 30 95–105Google Scholar
Exterkate, F. A. 1985 A dual-directed control of cell wall proteinase production in Streptococcus cremoris AM1: a possible mechanism of regulation during growth in milk. Journal of Dairy Science 68 562–571CrossRefGoogle Scholar
Exterkate, F. A. & De Veer, G. J. C. M. 1985 Partial isolation and degradation of caseins by cell wall proteinase(s) of Streptococcus cremoris HP. Applied and Environmental Microbiology 49 328–332CrossRefGoogle ScholarPubMed
Geis, A., Bockelmann, W. & Teuber, M. 1985 Simultaneous extraction and purification of a cell wall-associated peptidase and β-casein specific protease from Streptococcus cremoris AC1. Applied Microbiology and Biotechnology 23 79–84CrossRefGoogle Scholar
Hugenholtz, J., Exterkate, F. A. & Konings, W. N. 1984 The proteolytic system of Streptococcus cremoris: an immunological analysis. Applied and Environmental Microbiology 48 1105–1110CrossRefGoogle ScholarPubMed
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Merril, C. R., Goldman, D. & Van Keuren, M. L. 1983 Gel protein stains: silver stain. Methods in Enzymology 104 441–447CrossRefGoogle Scholar
Mills, O. E. & Thomas, T. D. 1978 Release of cell wall-associated proteinase(s) from lactic streptococci. New Zealand Journal of Dairy Science and Technology 13 209–215Google Scholar
Monnet, V., Le Bars, D. & Gripon, J. C. 1987 Partial characterization and comparison of cell wall proteinases from. 5 strains of Streptococcus lactis. Lait 67 51–61CrossRefGoogle Scholar
Rabier, D. & Desmazeaud, M. J. 1973 [Inventory of the different intracellular peptidase activities of Streptococcus thermophilus. Purification and properties of a dipeptide hydrolase and an aminopeptidase] Biochimie 55 389–404CrossRefGoogle Scholar
Terzaghi, B. E. & Sandine, W. E. 1975 Improved medium for lactic streptococci and their bacteriophages. Applied Microbiology 29 807–813CrossRefGoogle ScholarPubMed
Thomas, T. D. 1975 Tagatose-1,6-diphosphate activation of lactate dehydrogenase from Streptococcus cremoris. Biochemical and Biophysical Research Communications 63 1035–1042CrossRefGoogle ScholarPubMed
Thomas, T. D., Jarvis, B. D. W. & Skipper, N. A. 1974 Localization of proteinase(s) near the cell surface of Streptococcus lactis. Journal of Bacteriology 118 329–333CrossRefGoogle ScholarPubMed
Thomas, T. D. & Mills, O. E. 1981 Proteolytic enzymes of starter bacteria. Netherlands Milk and Dairy Journal 35 255–273Google Scholar
Thomas, T. D. & Turner, K. W. 1977 Preparation of skim milk to allow harvesting of starter cells from milk cultures. New Zealand Journal of Dairy Science and Technology 12 15–21Google Scholar