Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-23T03:00:59.291Z Has data issue: false hasContentIssue false
  • English
  • Français

Prolactin-stimulated polymerization of acetyl-CoA carboxylase in explants of mid-pregnant rat mammary gland

Published online by Cambridge University Press:  15 October 2001

JULIUS E. OBEN  and
RAYMOND R. DILS
JULIUS E. OBEN
Affiliation:
School of Animal and Microbial Sciences, The University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, UK Present address: Medex Scientific UK Ltd, Horsham, West Sussex, RH12 1JE, UK.
RAYMOND R. DILS
Affiliation:
School of Animal and Microbial Sciences, The University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Prolactin significantly increased the rate of fatty acid synthesis in explants of mid-pregnant rat mammary gland cultured for 96 h with insulin plus corticosterone. Under these conditions, prolactin increased the specific activity of total acetyl-CoA carboxylase in nuclear-free homogenates of explants by 2·6, and increased the proportion of the enzyme in the active polymeric form from 0·44 to 0·89. Removal of prolactin after 48 h in culture decreased the specific activity of the total enzyme by about half, and decreased the proportion as polymer to 0·52. The results show that prolactin plays a major role in mid-pregnant rat mammary gland in the polymerization which accompanies increased activity of the total enzyme and increased rate of fatty acid synthesis.

Keywords

Acetyl-CoA carboxylasepolymerisationmammary gland
Type
Original article
Information
Journal of Dairy Research , Volume 68 , Issue 3 , August 2001 , pp. 351 - 355
Copyright
Proprietors of Journal of Dairy Research 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)