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Production of methanethiol in milk fat-coated microcapsules containing Brevibacterium linens and methionine

Published online by Cambridge University Press:  01 June 2009

Sun C. Kim  and
Norman F. Olson
Sun C. Kim
Affiliation:
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA
Norman F. Olson
Affiliation:
Center for Dairy Research Department of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA
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Summary

Milk fat-coated microcapsules containing Brevibacterium linens and methionine were used to produce methanethiol, one of the volatile sulphur compounds implicated in Cheddar cheese flavour. Production of methanethiol from methionine occurred aerobically and anaerobically, but the production was 3- to 4-fold greater aerobically with most of the methanethiol being oxidized to dimethyl disulphide. About 35% of the total methanethiol was absorbed by the milk fat capsules and about 65% detected in the headspace. Levels of methanethiol began to decline at 26 °C after 24 h in milk fat-coated microcapsules. However, low temperature, such as 4–12 °C, stabilized levels of methanethiol in microcapsules over an 8-d analysis period. Optimum pH and temperature for methanethiol production were 8 and 26 °C respectively. The antioxidants butylated hydroxytoluene, butylated hydroxyanisole and ascorbic acid had negligible effects on methanethiol production.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 799 - 811
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

REFERENCES

Aston, J. W. & Dulley, J. R. 1982 Cheddar cheese flavour. Australian Journal of Dairy Technology 37 59–64Google Scholar
Badings, H. T., Maarse, H., Kleipool, R. J. C., Tas, A. C., Neeter, R. & Ten Noever De Brauw, M. C. 1975 Formation of odorous compounds from hydrogen sulphide and methanethiol, and unsaturated carbonyls. Proceedings, International Symposium on Aroma Research pp. 63–73 (Eds Maarse, H. and Groenen, P. J.) Wageningen, The Netherlands: PudocGoogle Scholar
Boyaval, P. & Desmazeaud, M. J. 1983 [Present knowledge about Brevibacterium linens.] Lait 63 187–216CrossRefGoogle Scholar
Braun, S. D. & Olson, N. F. 1986 Encapsulation of proteins and peptides in milk fat: encapsulation efficiency and temperature and freezing stabilities. Journal of Microencapsulation 3 115–126Google Scholar
Cole, M. 1969 Factors affecting the synthesis of ampicillin and hydroxypenicillins by the cell-bound penicillin acylase of Escherichia coli. Journal of Biochemistry 115 759–764Google Scholar
Cuer, A., Dauphin, G., Kergomard, A., Dumont, J. P. & Adda, J. 1979 Production of S-methylthioacetate by Brevibacterium linens. Applied and Environmental Microbiology 38 332–334CrossRefGoogle ScholarPubMed
De Souza, T. L. C., Lane, D. C. & Bhatia, S. P. 1975 Analysis of sulfur-containing gases by gas-solid chromatography on a specially treated Porapak QS column packing. Analytical Chemistry 47 543–545CrossRefGoogle Scholar
Hemme, D., Bouillane, C., Métro, F. & Desmazeaud, M. J. 1982 Microbial catabolism of amino acids during cheese ripening. Sciences des Aliments 2 113–123Google Scholar
Kim, S. C. & Olson, N. F. 1985 Characteristics of viable Brevibacterium linens cells containing methionine and cysteine in milkfat-coated microcapsules. Journal of Microencapsulation 2 197–206Google Scholar
Kim, S. C. & Olson, N. F. 1989 Production of hydrogen sulfide in milkfat-coated microcapsules containing Brevibacterium linens and cysteine. Journal of Microencapsulation 6 In PressCrossRefGoogle ScholarPubMed
Law, B. A. & Sharpe, M. E. 1977 The influence of the microflora of Cheddar cheese on flavour development. Dairy Industries International 42 (12) 10–14Google Scholar
Law, B. A. & Sharpe, M. E. 1978 Formation of methanethiol by bacteria isolated from raw milk and Cheddar cheese. Journal of Dairy Research 45 267–275CrossRefGoogle Scholar
Libbey, L. M. & Day, E. A. 1963 Methyl mercaptan as a component of Cheddar cheese. Journal of Dairy Science 46 859–861Google Scholar
Magee, E. L. & Olson, N. F. 1981 Microencapsulation of cheese ripening systems: formation of microcapsules. Journal of Dairy Science 64 600–610Google Scholar
Manning, D. J. 1974 Sulphur compounds in relation to Cheddar cheese flavour. Journal of Dairy Research 41 81–87CrossRefGoogle Scholar
Manning, D. J. 1979 a Cheddar cheese flavour studies. II. Relative flavour contributions of individual volatile components. Journal of Dairy Research 46 523–529CrossRefGoogle Scholar
Manning, D. J. 1979 b Chemical production of essential Cheddar flavour compounds. Journal of Dairy Research 46 531–537CrossRefGoogle Scholar
Manning, D. J., Chapman, H. R. & Hosking, Z. D. 1976 The production of sulphur compounds in Cheddar cheese and their significance in flavour development. Journal of Dairy Research 43 313–320CrossRefGoogle Scholar
Manning, D. J. & Moore, C. 1979 Headspace analysis of hard cheeses. Journal of Dairy Research 46 539–545CrossRefGoogle Scholar
Manning, D. J. & Price, J. C. 1977 Cheddar cheese aroma: the effect of selectively removing specific classes of compounds from cheese headspace. Journal of Dairy Research 44 357–361CrossRefGoogle Scholar
Manning, D. J. & Robinson, H. M. 1973 The analysis of volatile substances associated with Cheddar-cheese aroma. Journal of Dairy Research 40 63–75CrossRefGoogle Scholar
Rippe, J. K., Lindsay, R. C. & Olson, N. F. 1983 Butterfat encapsulation of a methanethiol producing enzyme system for acceleration of Cheddar cheese flavor development. Journal of Dairy Science 66 (Supplement) 77Google Scholar
Sharpe, M. E., Law, B. A. & Phillips, B. A. 1976 Coryneform bacteria producing methanethiol. Journal of General Microbiology 94 430–435CrossRefGoogle Scholar
Sharpe, M. E., Law, B. A., Phillips, B. A. & Pitcher, D. G. 1977 Methanethiol production by Coryneform bacteria: strains from dairy and human skin sources and Brevibacterium linens. Journal of General Microbiology 101 345–349CrossRefGoogle ScholarPubMed
Soda, K., Tanaka, H. & Esaki, N. 1983 Multifunctional biocatalysis–methionine γ-lyase. Trends in Biochemical Sciences 8 214–217CrossRefGoogle Scholar
Tanaka, H., Esaki, N. & Soda, K. 1977 Properties of L-methionine γ-lyase from Pseudomonas ovalis. Biochemistry 16 100–106Google Scholar
Tanaka, H., Esaki, N. & Soda, K. 1983 Bacterial L-methionine γ-lyase: characterization and application. In Sulfur Amino Acids: biochemical and clinical aspects pp. 365–377 (Eds Kuriyama, K., Huxtable, R. J. and Iwata, H.) New York: Alan R. Liss (Progress in Clinical and Biological Research 125)Google Scholar
Tanaka, H., Esaki, N., Yamamoto, T. & Soda, K. 1976 Purification and properties of methioninase from Pseudomonas ovalis. FEBS Letters 66 307–311CrossRefGoogle ScholarPubMed
Tokita, F. & Hosono, A. 1968 Production of volatile sulfur compounds by Brevibacterium linens. Japanese Journal of Zootechnical Science 39 127–132Google Scholar