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Prevalence of pathogens in milk samples of dairy cows with clinical mastitis and in heifers at first parturition

Published online by Cambridge University Press:  05 January 2009

Bernd-Alois Tenhagen ,
Inken Hansen ,
Annette Reinecke  and
Wolfgang Heuwieser
Bernd-Alois Tenhagen*
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin, Germany
Inken Hansen
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin, Germany
Annette Reinecke
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin, Germany
Wolfgang Heuwieser
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin, Germany
*
†For correspondence; e-mail: [email protected]
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Abstract

Prevalence of mastitis pathogens in milk samples from dairy cows and heifers was studied over a period of 1 year (Aug 2005–Aug 2006) in ten dairy herds in Germany. Milk samples (n=8240) were collected from heifers without clinical mastitis at parturition (n=6915), from primiparous cows with clinical mastitis (n=751) and from older cows with clinical mastitis (n=574). Coagulase negative staphylococci (CNS) were the predominant group of bacteria isolated (46·8% of samples) from clinically healthy quarters of primiparous cows around parturition, followed by streptococci (12·6%), coliforms (4·7%) and Staphylococcus aureus (4·0%). Thirty-three percent of samples were negative on culture (range on farm level, 12·0–46·4%). In cases of clinical mastitis in primiparous and older cows, streptococci were the predominant finding (32·1 and 39·2%) followed by CNS (27·4 and 16·4%), coliforms (10·3 and 13·1%) and Staph. aureus (10·0 and 11·7%). Negative results were obtained from 21·3% (range, 0·0–30·6%) and 19·5% (range, 0·0–32·6%) of these samples. Results indicated substantial differences in the prevalence of pathogens among herds. There was a positive within-herd correlation between the monthly prevalences for Streptococcus dysgalactiae between the three groups of samples. This correlation was also found between clinical samples of primiparous and older cows for Staph. aureus. These correlations were not found for the other pathogens. Besides herd, prevalence of pathogens was influenced by parity, type of sample and season.

Keywords

MastitisstreptococcistaphylococcicoliformsStaphylococcus aureusStreptococcus dysgalactiae
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 179 - 187
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Aarestrup, FM & Jensen, NE 1997 Prevalence and duration of intramammary infection in Danish heifers during the peripartum period. Journal of Dairy Science 80 307–312CrossRefGoogle ScholarPubMed
Barker, AR, Schrick, FN, Lewis, MJ, Dowlen, HH & Oliver, SP 1998 Influence of clinical mastitis during early lactation on reproductive performance of Jersey cows. Journal of Dairy Science 81 1285–1290CrossRefGoogle ScholarPubMed
Bassel, L, Kelton, D, Godkin, A & Leslie, KE 2003 Risk factors for intramammary infection at first calving in Ontario dairy heifers. American Association of Bovine Practitioners Proceedings 36 177–178Google Scholar
Borm, AA, Fox, LK, Leslie, KE, Hogan, JS, Andrew, SM, Moyes, KM, Oliver, SP, Schukken, YH, Hancock, DD, Gaskins, CT, Owens, WE & Norman, C 2006 Effects of prepartum intramammary antibiotic therapy on udder health, milk production, and reproductive performance in dairy heifers. Journal of Dairy Science 89 2090–2098CrossRefGoogle Scholar
Calvinho, LF, Canavesio, VR, Iguzquiza, IA, Marioni, I, Puricelli, FG, Neder, VE, Tarabla, HD & Aubagna, MD 2007 Intramammary infections during the periparturient period in Argentine dairy heifers. Revista Argentina Microbiologia 39 84–89Google ScholarPubMed
Calvinho, LF & Oliver, SP 1998 Invasion and persistence of Streptococcus dysgalactiae within bovine mammary epithelial cells. Journal of Dairy Science 81 678–686CrossRefGoogle ScholarPubMed
Compton, CW, Heuer, C, Parker, K & McDougall, S 2007 Epidemiology of mastitis in pasture-grazed peripartum dairy heifers and its effects on productivity. Journal of Dairy Science 90 4157–4170CrossRefGoogle ScholarPubMed
De Vliegher, S, Laevens, H, Devriese, LA, Opsomer, G, Leroy, JL, Barkema, HW & de Kruif, A 2003 Prepartum teat apex colonization with Staphylococcus chromogenes in dairy heifers is associated with low somatic cell count in early lactation. Veterinary Microbiology 92 245–252CrossRefGoogle ScholarPubMed
Edinger, D, Tenhagen, BA, Heuwieser, W, Kalbe, P, Klunder, G & Baumgartner, B 1999 Effect of puerperal mastitis in primiparous cows on milk production, cell count and culling. Deutsche TierärztlicheWochenschrift 106 470–474Google ScholarPubMed
Edinger, D, Tenhagen, BA, Kalbe, P, Klunder, G, Baumgartner, B & Heuwieser, W 2000 Effect of teat dipping with a germicide barrier teat dip in late gestation on intramammary infection and clinical mastitis during the first 5 days post-partum in primiparous cows. Journal of Veterinary Medicine A 47 463–468CrossRefGoogle ScholarPubMed
IDF 1999 Suggested interpretation of mastitis terminology. Bulletin of the IDF 338 3–26Google Scholar
Jonsson, P, Olsson, SO, Olofson, AS, Falth, C, Holmberg, O & Funke, H 1991 Bacteriological investigations of clinical mastitis in heifers in Sweden. Journal of Dairy Research 58 179–185CrossRefGoogle ScholarPubMed
Kalmus, P, Viltrop, A, Aasmae, B & Kask, K 2006 Occurrence of clinical mastitis in primiparous Estonian dairy cows in different housing conditions. Acta Veterinaria Scandinavia 48 21–26CrossRefGoogle ScholarPubMed
Madsen, M, Aalbaek, B & Hansen, JW 1992 Comparative bacteriological studies on summer mastitis in grazing cattle and pyogenes mastitis in stabled cattle in Denmark. Veterinary Microbiology 32 81–88CrossRefGoogle ScholarPubMed
Oliver, SP, Headrick, SI, Gillespie, BE, Lewis, MJ, Johnson, DL, Lamar, KC, Moorehead, H, Dowlen, HH & Hallberg, JW 2007 Intramammary infections in heifers during early lactation following intramammary infusion of pirlimycin hydrochloride or penicillin-novobiocin at the first milking after parturition. Journal of Dairy Research 74 211–217CrossRefGoogle ScholarPubMed
Oliver, SP, Lewis, MJ, Gillespie, BE & Dowlen, HH 1997 Antibiotic residues and prevalence of mastitis pathogen isolation in heifers during early lactation following prepartum antibiotic therapy. Zentralblatt für Veterinärmedizin B 44 213–220Google ScholarPubMed
Østerås, O, Solverod, L & Reksen, O 2006 Milk culture results in a large Norwegian survey – effects of season, parity, days in milk, resistance, and clustering. Journal of Dairy Science 89 1010–1023CrossRefGoogle Scholar
Parker, KI, Compton, C, Anniss, FM, Weir, A, Heuer, C & McDougall, S 2007a Subclinical and clinical mastitis in heifers following the use of a teat sealant precalving. Journal of Dairy Science 90 207–218CrossRefGoogle ScholarPubMed
Parker, KI, Compton, CW, Anniss, FM, Weir, AM & McDougal, S 2007b Management of dairy heifers and its relationships with the incidence of clinical mastitis. New Zealand Veterinary Journal 55 208–216CrossRefGoogle ScholarPubMed
Roberson, JR, Fox, LK, Hancock, DD, Gay, JM & Besser, TE 1998 Sources of intramammary infections from Staphylococcus aureus in dairy heifers at first parturition. Journal of Dairy Science 81 687–693CrossRefGoogle ScholarPubMed
Sargeant, JM, Scott, HM, Leslie, KE, Ireland, MJ & Bashiri, A 1998 Clinical mastitis in dairy cattle in Ontario: frequency of occurrence and bacteriological isolates. Canadian Veterinary Journal 39 33–38Google ScholarPubMed
Shpigel, NY, Winkler, M, Ziv, G & Saran, A 1998 Clinical, bacteriological and epidemiological aspects of clinical mastitis in Israeli dairy herds. Preventive Veterinary Medicine 35 1–9CrossRefGoogle ScholarPubMed
Smith, KL, Todhunter, DA & Schoenberger, PS 1985 Environmental mastitis: cause, prevalence, prevention. Journal of Dairy Science 68 1531–1553CrossRefGoogle ScholarPubMed
Tenhagen, BA, Edinger, D, Baumgartner, B, Kalbe, P, Klunder, G & Heuwieser, W 2001 Efficacy of a herd-specific vaccine against Staphylococcus aureus to prevent post-partum mastitis in dairy heifers. Journal of Veterinary Medicine A 48 601–607CrossRefGoogle ScholarPubMed
Tenhagen, BA, Koster, G, Wallmann, J & Heuwieser, W 2006 Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Journal of Dairy Science 89 2542–2551CrossRefGoogle ScholarPubMed
Timms, LL & Schultz, LH 1987 Dynamics and significance of coagulase-negative staphylococcal intramammary infections. Journal of Dairy Science 70 2648–2657CrossRefGoogle ScholarPubMed
Todhunter, DA, Smith, KL & Hogan, JS 1995 Environmental streptococcal intramammary infections of the bovine mammary gland. Journal of Dairy Science 78 2366–2374CrossRefGoogle ScholarPubMed
Waage, S, Mork, T, Roros, A, Aasland, D, Hunshamar, A & Odegaard, SA 1999 Bacteria associated with clinical mastitis in dairy heifers. Journal of Dairy Science 82 712–719CrossRefGoogle ScholarPubMed
Waage, S, Odegaard, SA, Lund, A, Brattgjerd, S & Rothe, T 2001 Case-control study of risk factors for clinical mastitis in post-partum dairy heifers. Journal of Dairy Science 84 392–399CrossRefGoogle ScholarPubMed
Waage, S, Sviland, S & Odegaard, SA 1998 Identification of risk factors for clinical mastitis in dairy heifers. Journal of Dairy Science 81 1275–1284CrossRefGoogle ScholarPubMed