Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2025-01-01T14:37:09.941Z Has data issue: false hasContentIssue false
  • English
  • Français

Polyclonal antibodies to human milk caseins

Published online by Cambridge University Press:  01 June 2009

Marta Šimíčková ,
Bohumír A. Lang  and
Ladislav Slepička
Marta Šimíčková
Affiliation:
Institutes of Medical Research, Research Institute of Clinical and Experimental Oncology, 656 53 Brno, Czechoslovakia
Bohumír A. Lang
Affiliation:
Institutes of Medical Research, Research Institute of Clinical and Experimental Oncology, 656 53 Brno, Czechoslovakia
Ladislav Slepička
Affiliation:
Institute of Sera and Vaccines, 775 00 Olomouc, Czechoslovakia
Get access Rights & Permissions [Opens in a new window]

Summary

Preparations of total human casein and its individual fractions were isolated for production of specific polyclonal antibodies. Immunization procedures used differed in the schedule for antigen administration, antigen concentrations, degree of additional purification, and modification of the size of molecules. Immunoprecipitation techniques failed to provide an unambiguous proof of the presence of antibodies to human milk caseins in antisera even after immunization of animals phylogenetically considerably different from man. Immunoblotting, however, allowed antibodies to β-casein to be identified and compared with available monoclonal antibodies.

Type
Original articles
Information
Journal of Dairy Research , Volume 58 , Issue 1 , February 1991 , pp. 115 - 125
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Al-Sarraj, K., White, D. A. & Mayer, R. J. 1978 Immunochemical characterization of casein from rabbit mammary gland. Biochemical Journal 173 877–883CrossRefGoogle ScholarPubMed
Anderson, N. G., Powers, M. T. & Tollaksen, S. L. 1982 Proteins of human milk. 1. Identification of major components. Clinical Chemistry 28 1045–1055CrossRefGoogle Scholar
Bitter, T. & Muir, H. M. 1962 A modified uranic acid carbazole reaction. Analytical Biochemistry 4 330–334CrossRefGoogle Scholar
Bläckberg, L. & Hernell, O. 1980 Isolation of lactoferrin from human whey by a single Chromatographic step. FEBS Letters 109 180–184CrossRefGoogle ScholarPubMed
Burchell, J., Bartek, J. & Taylor-Papadimitriou, J. 1985 Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and α-lactalbumin. Hybridoma 4 341–350CrossRefGoogle ScholarPubMed
Chtourou, A., Brignon, G. & Ribadeau-Dumas, B. 1985 Quantification of β-Casein in human milk. Journal of Dairy Research 52 239–247CrossRefGoogle ScholarPubMed
Dayal, R., Hurlimann, J., Suard, Y. M. L. & Kraehenbuhl, J.-P. 1982 Chemical and immunochemical characterization of caseins and the major whey proteins of rabbit milk. Biochemical Journal 201 71–79CrossRefGoogle ScholarPubMed
Enami, J. & Nandi, S. 1977 A sensitive radioimmunoassay for a component of mouse casein. Journal of Immunological Methods 18 235–244CrossRefGoogle ScholarPubMed
Farr, A. G. & Nakane, P. K. 1981 Immunohistochemistry with enzyme labeled antibodies: a brief review. Journal of Immunological Methods 47 129–144CrossRefGoogle ScholarPubMed
Fortt, R. W., Gibbs, A. R., Williams, D., Hansen, J. & Williams, I. 1979 The identification of ‘casein’ in human breast cancer. Histopathology 3 395–406CrossRefGoogle ScholarPubMed
Goldfarb, M. F., Savadove, M. S. & Inman, J. A. 1989 Two-dimensional electrophoretic analysis of human milk proteins. Electrophoresis 10 67–70CrossRefGoogle ScholarPubMed
Greenberg, R. & Groves, M. L., 1984 Plasmin cleaves human β-casein. Biochemical and Biophysical Research Communications 125 463–468CrossRefGoogle ScholarPubMed
Habeeb, A. F. S. A. 1981 Enzymatic and immunochemical properties of lysozyme restriction of recognition of lysozyme antigenic determinants in pigs. Archives of Biochemistry and Biophysics 212 618–628CrossRefGoogle ScholarPubMed
Hartley, B. S. 1970 Strategy and tactics in protein chemistry. Biochemical Journal 119 805822CrossRefGoogle ScholarPubMed
Hendrick, J. C. & Franchimont, P. 1974 Radio-immunoassay of casein in the serum of normal subjects and of patients with various malignancies. European Journal of Cancer 10 725–730CrossRefGoogle ScholarPubMed
Koike, M., Tsuda, N., Ishii, T., Kashii, H. & Kodama, T. 1978 Fractionation of human breast milk casein by Sephadex G-200 column chromatography and calcium ion precipitation, and the physico-chemical properties of the fractions. Wakayama Medical Reports 21 1–13Google Scholar
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Meun, D. H. C. & Smith, K. C. 1968 A micro phosphate method. Analytical Biochemistry 26 364–368CrossRefGoogle ScholarPubMed
Monaco, M. E., Bronzert, D. A., Tormey, D. C., Waalkes, P. & Lippman, M. E. 1977 Casein production by human breast cancer. Cancer Research 37 749–754Google ScholarPubMed
Ormerod, M. G., Bussolati, G., Sloane, J. P., Steele, K. & Gugliotta, P. 1982 Similarities of antisera to casein and epithelial membrane antigen. Virchows Archiv. A. Pathological Anatomy and Histology 397 327–333CrossRefGoogle ScholarPubMed
Otani, H., Higashiyama, S. & Tokita, F. 1984 Antigenic reactivity of human casein with antiserum to bovine β-casein. Milchwissenschaft 39 65–67Google Scholar
Peterson, G. L. 1977 A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83 346–356CrossRefGoogle Scholar
Šimíčková, M., Lang, B. A., Semotán, K. & Kočent, A. 1987 A non-competitive enzyme immunoassay of human α-lactalbumin in biological fluids and tissue extracts. Clinica Chimica Acta 163 257–265CrossRefGoogle ScholarPubMed
Soulier, S., Ribadeau-Dumas, B. & Denamur, R. 1975 [Purification of κ-caseins from sheep. Analysis of the glycan and peptide components.] European Journal of Biochemistry 50 445–452CrossRefGoogle ScholarPubMed
Warren, L. 1959 The thiobarbituric acid assay of sialic acids. Journal of Biological Chemistry 234 1971–1975CrossRefGoogle ScholarPubMed
Weir, H. M., Mackinlay, A. G., Nash, A. R. & Sarfaty, G. A. 1981 Human kappa-casein as a tumor marker: purification and properties. Cancer Detection and Prevention 4 193–204Google Scholar
Woods, R. L., Searle, F., Wilson, P., Bagshawe, K. D. & Cooper, E. H. 1977 Serum kappa-casein in breast cancer. European Journal of Cancer 13 1113–1117CrossRefGoogle ScholarPubMed
Yoshikawa, M., Takahata, K., Sasaki, R. & Chiba, H. 1978 Aggregation of casein micelle from bovine milk by wheat germ lectin. Agricultural and Biological Chemistry 42 1923–1926Google Scholar
Zanetta, J. P., Vincendon, G., Mandel, P. & Gombos, G. 1970 The utilization of 1-dimethyl-aminonaphthalene-5-sulphonyl chloride for quantitative determination of free amino acids and partial analysis of primary structure of proteins. Journal of Chromatography 51 441–458CrossRefGoogle ScholarPubMed