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Phylogenetic genotyping, virulence genes and antimicrobial susceptibility of Escherichia coli isolates from cases of bovine mastitis

Published online by Cambridge University Press:  24 February 2021

Nashmil Aslam ,
Saeed-Ul-Hassan Khan ,
Tahir Usman  and
Tariq Ali
Nashmil Aslam
Affiliation:
Department of Zoology, Quaid-i-Azam University, Islamabad, Pakistan
Saeed-Ul-Hassan Khan
Affiliation:
Department of Zoology, Quaid-i-Azam University, Islamabad, Pakistan
Tahir Usman
Affiliation:
China Agricultural University, Beijing, China College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan
Tariq Ali*
Affiliation:
China Agricultural University, Beijing, China Centre of Microbiology and Biotechnology, Veterinary Research Institute, Peshawar, Pakistan
*
Author for correspondence: Tariq Ali, Email: [email protected]
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Abstract

The study described in this research communication used phylogenetic genotyping to identify virulence genes and antimicrobial susceptibility in Escherichia coli recovered from cases of bovine mastitis. From 385 mastitic milk samples, 30 (7.8%) isolates were confirmed as E. coli. Most isolates (80%) belonged to phylo-group A. These 30 E. coli isolates were also screened for 11 different virulence genes. The majority of isolates (63%) harbored no virulence gene. Only 11 (37%) isolates tested positive for two virulence genes, either the iron uptake gene iucD in 3 (10%) isolates or the serum resistance gene traT in 2 (7%) isolates or both traT and iucD in 6 (20%) isolates. The E. coli isolates showed highest susceptibility to gentamicin, meropenem, and pipracillin. Most isolates were resistant to ampicillin, cefotaxime and streptomycin. This study suggests that mastitis causing E. coli might originate from commensal bacteria and that the presence of these virulence genes, common in extra-intestinal pathogenic E. coli (ExPEC) strains could be attributed to high genetic variability of mastitis-causing E. coli.

Keywords

Antimicrobial susceptibilitybovine mastitisE. colivirulence genes
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 1 , February 2021 , pp. 78 - 79
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Ali, T, ur Rahman, S, Zhang, L, Shahid, M, Zhang, S, Liu, G, Gao, J and Han, B (2016) ESBL-producing Escherichia coli from cows suffering mastitis in China contain clinical class 1 integrons with CTX-M linked to ISCR1. Frontiers in Microbiology 7, 1931.CrossRefGoogle ScholarPubMed
Clermont, O, Bonacorsi, S and Bingen, E (2000) Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and Environmental Microbiology 66, 4555–4558.CrossRefGoogle ScholarPubMed
Cruz-Soto, AS, Toro-Castillo, V, Munguía-Magdaleno, CO, Torres-Flores, JE, Flores-Pantoja, LE, Loeza-Lara, PD and Jiménez-Mejía, R (2020) Genetic relationships, biofilm formation, motility and virulence of Escherichia coli isolated from bovine mastitis. Revista Mexicana De Ciencias Pecuarias 11, 167–182.CrossRefGoogle Scholar
Kaipainen, T, Pohjanvirta, T, Shpigel, NY, Shwimmer, A, Pyörälä, S and Pelkonen, S (2002) Virulence factors of Escherichia coli isolated from bovine clinical mastitis. Veterinary Microbiology 85, 37–46.CrossRefGoogle ScholarPubMed
Lan, T, Liu, H, Meng, L, Xing, M, Dong, L, Gu, M and Zheng, N (2020) Antimicrobial susceptibility, phylotypes, and virulence genes of Escherichia coli from clinical bovine mastitis in five provinces of China. Food and Agricultural Immunology 31, 406–423.CrossRefGoogle Scholar
Marashifard, M, Aliabad, ZK, Hosseini, SA, Darban-Sarokhalil, D, Mirzaii, M and Khoramrooz, SS (2019) Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran. Tropical Animal Health and Production 51, 575–580.CrossRefGoogle ScholarPubMed
Paton, AW and Paton, JC (2002) Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA And saa. Journal of Clinical Microbiology 40, 271–274.CrossRefGoogle ScholarPubMed
Suojala, L, Pohjanvirta, T, Simojoki, H, Myllyniemi, AL, Pitkälä, A, Pelkonen, S and Pyörälä, S (2011) Phylogeny, virulence factors and antimicrobial susceptibility of Escherichia coli isolated in clinical bovine mastitis. Veterinary Microbiology 147, 383–388.CrossRefGoogle ScholarPubMed
Zhang, D, Zhang, Z, Huang, C, Gao, X, Wang, Z, Liu, Y and Liu, M (2018) The phylogenetic group, antimicrobial susceptibility, and virulence genes of Escherichia coli from clinical bovine mastitis. Journal of Dairy Science 101, 572–580.CrossRefGoogle ScholarPubMed